BMRBj - BMREntryMaster

Found 1 Document (0.81 seconds)

BMRB: 17196

2L3M

Solution structure of the putative copper-ion-binding protein from Bacillus anthracis str. Ames

Authors

Zhang, Y., Winsor, J., Dubrovska, I., Anderson, W., Radhakrishnan, I., CSGID, C.

Assembly

Cu-binding protein

Entity

1. Cu-binding protein (polymer, Thiol state: not reported), 71 monomers, 7579.431 Da Detail

SNAMEQLTLQ VEGMSCGHCV NAIESSVKEL NGVEQVKVQL AEGTVEVTID SSVVTLKDIV AVIEDQGYDV Q

Formula weight

7579.431 Da

Source organism

Bacillus anthracis

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.2 %, Completeness (bb): 97.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.2 % (740 of 769)	96.2 % (376 of 391)	97.3 % (290 of 298)	92.5 % (74 of 80)
Backbone	97.4 % (415 of 426)	98.0 % (144 of 147)	97.6 % (203 of 208)	95.8 % (68 of 71)
Sidechain	95.6 % (391 of 409)	95.1 % (232 of 244)	98.1 % (153 of 156)	66.7 % (6 of 9)
Aromatic	75.0 % (9 of 12)	83.3 % (5 of 6)	66.7 % (4 of 6)
Methyl	100.0 % (104 of 104)	100.0 % (52 of 52)	100.0 % (52 of 52)

1. Cu-binding protein

SNAMEQLTLQ VEGMSCGHCV NAIESSVKEL NGVEQVKVQL AEGTVEVTID SSVVTLKDIV AVIEDQGYDV Q

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 K, pH 6.9

#	Name	Isotope labeling	Concentration
1	Cu-binding protein	[U-13C; U-15N]	1.1 mM
2	potassium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	beta-mercaptoethanol	natural abundance	1 mM
5	EDTA	natural abundance	1 mM
6	Protease Inhibitors	natural abundance	trace na
7	sodium azide	natural abundance	1 mM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 288 K, pH 6.9

#	Name	Isotope labeling	Concentration
10	Cu-binding protein	[U-13C; U-15N]	0.9 mM
11	potassium phosphate	natural abundance	20 mM
12	sodium chloride	natural abundance	100 mM
13	beta-mercaptoethanol	natural abundance	1 mM
14	EDTA	natural abundance	1 mM
15	Protease Inhibitors	natural abundance	trace na
16	sodium azide	natural abundance	1 mM
17	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.2 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L3M, Strand ID: A Detail

Release date

2012-07-31

Citation

To be published
Zhang, Y., Winsor, J., Dubrovska, I., Anderson, W., Radhakrishnan, I., CSGID, C.
Not known

Related entities 1. Cu-binding protein, : 1 : 347 entities Detail

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 K, pH 6.9

#	Name	Isotope labeling	Concentration
1	Cu-binding protein	[U-13C; U-15N]	1.1 mM
2	potassium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	beta-mercaptoethanol	natural abundance	1 mM
5	EDTA	natural abundance	1 mM
6	Protease Inhibitors	natural abundance	trace na
7	sodium azide	natural abundance	1 mM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

2 3D HNCACB

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 K, pH 6.9

#	Name	Isotope labeling	Concentration
1	Cu-binding protein	[U-13C; U-15N]	1.1 mM
2	potassium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	beta-mercaptoethanol	natural abundance	1 mM
5	EDTA	natural abundance	1 mM
6	Protease Inhibitors	natural abundance	trace na
7	sodium azide	natural abundance	1 mM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

3 3D CBCA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 K, pH 6.9

#	Name	Isotope labeling	Concentration
1	Cu-binding protein	[U-13C; U-15N]	1.1 mM
2	potassium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	beta-mercaptoethanol	natural abundance	1 mM
5	EDTA	natural abundance	1 mM
6	Protease Inhibitors	natural abundance	trace na
7	sodium azide	natural abundance	1 mM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

4 3D 1H-15N NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 K, pH 6.9

#	Name	Isotope labeling	Concentration
1	Cu-binding protein	[U-13C; U-15N]	1.1 mM
2	potassium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	beta-mercaptoethanol	natural abundance	1 mM
5	EDTA	natural abundance	1 mM
6	Protease Inhibitors	natural abundance	trace na
7	sodium azide	natural abundance	1 mM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

5 3D HCCH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 288 K, pH 6.9

#	Name	Isotope labeling	Concentration
10	Cu-binding protein	[U-13C; U-15N]	0.9 mM
11	potassium phosphate	natural abundance	20 mM
12	sodium chloride	natural abundance	100 mM
13	beta-mercaptoethanol	natural abundance	1 mM
14	EDTA	natural abundance	1 mM
15	Protease Inhibitors	natural abundance	trace na
16	sodium azide	natural abundance	1 mM
17	D2O	natural abundance	100 %

6 3D 1H-13C NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 288 K, pH 6.9

#	Name	Isotope labeling	Concentration
10	Cu-binding protein	[U-13C; U-15N]	0.9 mM
11	potassium phosphate	natural abundance	20 mM
12	sodium chloride	natural abundance	100 mM
13	beta-mercaptoethanol	natural abundance	1 mM
14	EDTA	natural abundance	1 mM
15	Protease Inhibitors	natural abundance	trace na
16	sodium azide	natural abundance	1 mM
17	D2O	natural abundance	100 %

7 3D HCCH-COSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 288 K, pH 6.9

#	Name	Isotope labeling	Concentration
10	Cu-binding protein	[U-13C; U-15N]	0.9 mM
11	potassium phosphate	natural abundance	20 mM
12	sodium chloride	natural abundance	100 mM
13	beta-mercaptoethanol	natural abundance	1 mM
14	EDTA	natural abundance	1 mM
15	Protease Inhibitors	natural abundance	trace na
16	sodium azide	natural abundance	1 mM
17	D2O	natural abundance	100 %

nullKeywords Protein

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (0.81 seconds)

BMRB: 17196

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Cu-binding protein, : 1 : 347 entities Detail

Experiments performed 7 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample