BMRBj - BMREntryMaster

Found 1 Document (1.637 seconds)

BMRB: 17202

2L3T

Solution structure of tandem SH2 domain from Spt6

Authors

Liu, J., Zhang, J., Wu, J., Shi, Y.

Assembly

Tandem SH2 domains of Spt6

Entity

1. Tandem SH2 domains of Spt6 (polymer, Thiol state: all free), 199 monomers, 23742.61 Da Detail

THRVINHPYY FPFNGRQAED YLRSKERGEF VIRQSSRGDD HLVITWKLDK DLFQHIDIQE LEKENPLALG KVLIVDNQKY NDLDQIIVEY LQNKVRLLNE MTSSEKFKSG TKKDVVKFIE DYSRVNPNKS VYYFSLNHDN PGWFYLMFKI NANSKLYTWN VKLTNTGYFL VNYNYPSVIQ LCNGFKTLLK SLEHHHHHH

Formula weight

23742.61 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.0 %, Completeness: 78.9 %, Completeness (bb): 92.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	78.9 % (1970 of 2496)	85.1 % (1110 of 1304)	66.8 % (648 of 970)	95.5 % (212 of 222)
Backbone	92.1 % (1089 of 1182)	93.3 % (373 of 400)	89.6 % (528 of 589)	97.4 % (188 of 193)
Sidechain	69.8 % (1051 of 1505)	81.6 % (738 of 904)	50.5 % (289 of 572)	82.8 % (24 of 29)
Aromatic	36.1 % (106 of 294)	63.9 % (94 of 147)	6.9 % (10 of 144)	66.7 % (2 of 3)
Methyl	72.3 % (149 of 206)	96.1 % (99 of 103)	48.5 % (50 of 103)

1. Tandem SH2 domains of Spt6

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	0.5 mM
2	potassium phosphate	natural abundance	25 mM
3	sodium chloride	natural abundance	150 mM
4	EDTA	natural abundance	1 mM
5	DTT	natural abundance	1 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.658 ppm	internal	indirect	0.2514495
¹H	water	protons	4.658 ppm	internal	direct	1.0
¹⁵N	water	protons	4.658 ppm	internal	indirect	0.1013291

Referencing offset: -0.22 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.658 ppm	internal	indirect	0.2514495
¹H	water	protons	4.658 ppm	internal	direct	1.0
¹⁵N	water	protons	4.658 ppm	internal	indirect	0.1013291

Referencing offset: -0.22 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.658 ppm	internal	indirect	0.2514495
¹H	water	protons	4.658 ppm	internal	direct	1.0
¹⁵N	water	protons	4.658 ppm	internal	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.658 ppm	internal	indirect	0.2514495
¹H	water	protons	4.658 ppm	internal	direct	1.0
¹⁵N	water	protons	4.658 ppm	internal	indirect	0.1013291

Referencing offset: 0.31 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L3T, Strand ID: A Detail

Release date

2011-06-20

Citation

Solution structure of tandem SH2 domains from Spt6 protein and their binding to the phosphorylated RNA polymerase II C-terminal domain
Liu, J., Zhang, J., Gong, Q., Xiong, P., Huang, H., Wu, B., Lu, G., Wu, J., Shi, Y.
J. Biol. Chem. (2011), 286, 29218-29226, PubMed 21676864 , DOI 10.1074/jbc.M111.252130 , Abstract

Related entities 1. Tandem SH2 domains of Spt6, : 1 : 9 : 47 entities Detail

Interaction partners 1. Tandem SH2 domains of Spt6, : 27 interactors Detail

More details for 27 interactors identified by 10 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC