BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	90.4 % (718 of 794)	89.6 % (381 of 425)	92.0 % (275 of 299)	88.6 % (62 of 70)
Backbone	95.4 % (372 of 390)	96.3 % (129 of 134)	94.8 % (183 of 193)	95.2 % (60 of 63)
Sidechain	87.1 % (405 of 465)	86.6 % (252 of 291)	90.4 % (151 of 167)	28.6 % (2 of 7)
Aromatic	0.0 % (0 of 18)	0.0 % (0 of 9)	0.0 % (0 of 9)
Methyl	94.6 % (70 of 74)	94.6 % (35 of 37)	94.6 % (35 of 37)

1. Lrp DNA-binding domain

MVDSKKRPGK DLDRIDRNIL NELQKDGRIS NVELSKRVGL SPTPCLERVR RLERQGFIQG YTALLN

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Lrp DNA-binding domain	[U-99% 13C; U-99% 15N]	2 mM
2	potassium phosphate	natural abundance	50 mM
3	beta-mercaptoethanol	natural abundance	5 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Concentration
6	Lrp DNA-binding domian	[U-99% 13C; U-99% 15N]	2 mM
7	potassium phosphate	natural abundance	50 mM
8	DTT	natural abundance	2 mM
9	D2O	natural abundance	100 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Concentration
10	Lrp DNA-biinding domain	[U-99% 13C; U-99% 15N]	80.0 ~ 150.0 uM
11	potassium phosphate	natural abundance	30 mM
12	beta-mercaptoethanol	natural abundance	2 mM
13	potassium chloride	natural abundance	20 mM
14	H2O	natural abundance	90 %
15	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.17 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.17 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.22 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 26 models in PDB: 2L4A, Strand ID: A Detail

Release date

2011-02-23

Citation

The design involved in PapI and Lrp regulation of the pap operon
Kawamura, T., Vartanian, A.S., Zhou, H., Dahlquist, F.W.
J. Mol. Biol. (2011), 409, 311-332, PubMed 21338611 , DOI 10.1016/j.jmb.2011.01.058 , Abstract

Related entities 1. Lrp DNA-binding domain, : 1 : 4 : 2 : 83 entities Detail

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Lrp DNA-binding domain	[U-99% 13C; U-99% 15N]	2 mM
2	potassium phosphate	natural abundance	50 mM
3	beta-mercaptoethanol	natural abundance	5 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

2 2D 1H-13C HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Lrp DNA-binding domain	[U-99% 13C; U-99% 15N]	2 mM
2	potassium phosphate	natural abundance	50 mM
3	beta-mercaptoethanol	natural abundance	5 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

3 3D CBCA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Lrp DNA-binding domain	[U-99% 13C; U-99% 15N]	2 mM
2	potassium phosphate	natural abundance	50 mM
3	beta-mercaptoethanol	natural abundance	5 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

4 3D C(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Lrp DNA-binding domain	[U-99% 13C; U-99% 15N]	2 mM
2	potassium phosphate	natural abundance	50 mM
3	beta-mercaptoethanol	natural abundance	5 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

5 3D HCCH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Lrp DNA-binding domain	[U-99% 13C; U-99% 15N]	2 mM
2	potassium phosphate	natural abundance	50 mM
3	beta-mercaptoethanol	natural abundance	5 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

6 3D HNCACB

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Lrp DNA-binding domain	[U-99% 13C; U-99% 15N]	2 mM
2	potassium phosphate	natural abundance	50 mM
3	beta-mercaptoethanol	natural abundance	5 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

7 4D 15N-13C edited NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Lrp DNA-binding domain	[U-99% 13C; U-99% 15N]	2 mM
2	potassium phosphate	natural abundance	50 mM
3	beta-mercaptoethanol	natural abundance	5 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

8 4D 13C-131C edited NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Concentration
6	Lrp DNA-binding domian	[U-99% 13C; U-99% 15N]	2 mM
7	potassium phosphate	natural abundance	50 mM
8	DTT	natural abundance	2 mM
9	D2O	natural abundance	100 %

9 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Concentration
10	Lrp DNA-biinding domain	[U-99% 13C; U-99% 15N]	80.0 ~ 150.0 uM
11	potassium phosphate	natural abundance	30 mM
12	beta-mercaptoethanol	natural abundance	2 mM
13	potassium chloride	natural abundance	20 mM
14	H2O	natural abundance	90 %
15	D2O	natural abundance	10 %

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BMRB: 17234

Sample #1

Sample #2

Sample #3

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Lrp DNA-binding domain, : 1 : 4 : 2 : 83 entities Detail

Experiments performed 9 experiments Detail

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