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BMRB: 17235

2L4B

Solution structure of a putative acyl carrier protein from Anaplasma phagocytophilum. Seattle Structural Genomics Center for Infectious Disease (SSGCID) target AnphA.01018.a

Authors

Buchko, G.W.

Assembly

ACP18

Entity

1. ACP18 (polymer, Thiol state: all free), 88 monomers, 9775.868 Da Detail

PGSMVSEEIK AQVMESVIGC LKLNDEQKQI LSGTTNLAKD FNLDSLDFVD LIMSLEERFS LEISDEDAQK LETVDDICRY IASKSSDA

Formula weight

9775.868 Da

Source organism

Anaplasma phagocytophilum

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.0 %, Completeness (bb): 97.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	95.0 % (948 of 998)	93.0 % (481 of 517)	96.4 % (373 of 387)	100.0 % (94 of 94)
Backbone	97.3 % (512 of 526)	97.8 % (174 of 178)	96.2 % (251 of 261)	100.0 % (87 of 87)
Sidechain	93.2 % (519 of 557)	90.6 % (307 of 339)	97.2 % (205 of 211)	100.0 % (7 of 7)
Aromatic	100.0 % (38 of 38)	100.0 % (19 of 19)	100.0 % (19 of 19)
Methyl	94.2 % (98 of 104)	92.3 % (48 of 52)	96.2 % (50 of 52)

1. ACP18

PGSMVSEEIK AQVMESVIGC LKLNDEQKQI LSGTTNLAKD FNLDSLDFVD LIMSLEERFS LEISDEDAQK LETVDDICRY IASKSSDA

Show sample condition

Sample #1

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 293 K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
1	ACP18	[U-100% 13C; U-100% 15N]	1.0 ~ 2.0 mM
2	sodium chloride	natural abundance	100 (±3.0) mM
3	TRIS	natural abundance	20 (±1.0) mM
4	H2O	natural abundance	93 %
5	D2O	natural abundance	7 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
6	ACP18	[U-98% 15N]	1.0 ~ 2.0 mM
7	sodium chloride	natural abundance	100 (±3.0) mM
8	TRIS	natural abundance	20 (±1.0) mM
9	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.14 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.14 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L4B, Strand ID: A Detail

Release date

2010-10-19

Citation

Solution structure of a putative acyl carrier protein from Anaplasma phagocytophilum, the bacterium responsible for human granulocytic ehrlichiosis
Buchko, G.W., Hewitt, S.N., Napuli, A.J., Van Voorhis, W.C., Myler, P.J.
Not known

Related entities 1. ACP18, : 1 : 150 entities Detail

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC