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BMRB: 17236

2L4D

cytochrome c domain of pp3183 protein from Pseudomonas putida

Authors

Banci, L., Bertini, I., Ciofi-Baffoni, S., Kozyreva, T., Mori, M., Wang, S.

Assembly

Sco proteins

Entity

1. cytochrome (polymer, Thiol state: all free), 110 monomers, 12273.85 Da Detail

GSFTSGEQIF RTRCSSCHTV GNTEPGQPGI GPDLLGVTRQ RDANWLVRWL KVPDQMLAEK DPLAMLLFEQ YNRLAMPNMR LGDAEVSALI SYLEEETARL QTPVTNRGIP

2. FE (III) ION (non-polymer), 55.845 Da
3. HEME C (non-polymer), 618.503 Da

Total weight

12948.197 Da

Max. entity weight

12273.85 Da

Source organism

Pseudomonas putida

Exptl. method

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 85.5 %, Completeness: 42.6 %, Completeness (bb): 72.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	42.6 % (539 of 1265)	23.8 % (157 of 661)	58.9 % (288 of 489)	81.7 % (94 of 115)
Backbone	72.7 % (468 of 644)	50.7 % (112 of 221)	81.9 % (263 of 321)	91.2 % (93 of 102)
Sidechain	20.9 % (151 of 722)	10.2 % (45 of 440)	39.0 % (105 of 269)	7.7 % (1 of 13)
Aromatic	2.7 % (2 of 74)	5.4 % (2 of 37)	0.0 % (0 of 35)	0.0 % (0 of 2)
Methyl	20.5 % (25 of 122)	8.2 % (5 of 61)	32.8 % (20 of 61)

1. cytochrome

GSFTSGEQIF RTRCSSCHTV GNTEPGQPGI GPDLLGVTRQ RDANWLVRWL KVPDQMLAEK DPLAMLLFEQ YNRLAMPNMR LGDAEVSALI SYLEEETARL QTPVTNRGIP

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	cytochrome c	[U-100% 13C; U-100% 15N]		0.8 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Type	Concentration
4	cytochrome c	[U-100% 15N]		0.8 mM
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

Protein Blocks Logo

Calculated from 20 models in PDB: 2L4D, Strand ID: A Detail

Release date

2011-01-17

Citation

Sco proteins are involved in electron transfer processes
Banci, L., Bertini, I., Ciofi-Baffoni, S., Kozyreva, T., Mori, M., Wang, S.
J. Biol. Inorg. Chem. (2011), 16, 391-403, PubMed 21181421 , DOI 10.1007/s00775-010-0735-x , Abstract

Related entities 1. cytochrome, : 1 : 86 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	cytochrome c	[U-100% 13C; U-100% 15N]		0.8 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	cytochrome c	[U-100% 13C; U-100% 15N]		0.8 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

3 3D CBCA(CO)NH

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	cytochrome c	[U-100% 13C; U-100% 15N]		0.8 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

4 3D HNCO

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	cytochrome c	[U-100% 13C; U-100% 15N]		0.8 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

5 3D HNCA

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	cytochrome c	[U-100% 13C; U-100% 15N]		0.8 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

6 3D HBHA(CO)NH

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	cytochrome c	[U-100% 13C; U-100% 15N]		0.8 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

7 3D HCCH-TOCSY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	cytochrome c	[U-100% 13C; U-100% 15N]		0.8 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

8 3D 1H-15N NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	cytochrome c	[U-100% 13C; U-100% 15N]		0.8 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

9 3D 1H-13C NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	cytochrome c	[U-100% 13C; U-100% 15N]		0.8 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

10 2D 1H-1H NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	cytochrome c	[U-100% 13C; U-100% 15N]		0.8 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

nullKeywords cytochrome c, electron transfer, nmr, sco