BMRB: 17237

NMR Structure of hFn14

Authors

Pellegrini, M., Willen, L., Perroud, M., Krushinskie, D., Strauch, K., Cuervo, H., Sun, Y., Day, E.S., Schneider, P., Zheng, T.S.

Assembly

hFn14

Entity

1. hFn14 (polymer, Thiol state: all disulfide bound), 53 monomers, 5609.340 Da Detail

EQAPGTAPCS RGSSWSADLD KCMDCASCRA RPHSDFCLGC AAAPPAPFRL LWP

Formula weight

5609.34 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS9:SG	1:CYS22:SG
2	disulfide	sing	1:CYS25:SG	1:CYS40:SG
3	disulfide	sing	1:CYS28:SG	1:CYS37:SG

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 69.8 %, Completeness: 57.5 %, Completeness (bb): 70.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	57.5 % (196 of 341)	55.8 % (163 of 292)	67.3 % (33 of 49)
Backbone	70.9 % (105 of 148)	70.6 % (72 of 102)	71.7 % (33 of 46)
Sidechain	47.2 % (91 of 193)	47.9 % (91 of 190)	0.0 % (0 of 3)
Aromatic	46.2 % (12 of 26)	50.0 % (12 of 24)	0.0 % (0 of 2)
Methyl	38.9 % (7 of 18)	38.9 % (7 of 18)

1. Fn14

EQAPGTAPCS RGSSWSADLD KCMDCASCRA RPHSDFCLGC AAAPPAPFRL LWP

Show sample condition

Sample #1

Solvent system 95% H2O, 5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details pH 7.4

#	Name	Isotope labeling	Type	Concentration
1	hFn14	[U-99% 15N]		700 uM
2	sodium phosphate	natural abundance		10 mM
3	sodium chloride	natural abundance		137 mM
4	potassium chloride	natural abundance		2.7 mM
5	H2O	natural abundance		95 %
6	D2O	natural abundance		5 %

Sample #2

Solvent system 95% H2O, 5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details pH 7.4

#	Name	Isotope labeling	Type	Concentration
7	hFn14	natural abundance		700 uM
8	sodium phosphate	natural abundance		10 mM
9	sodium chloride	natural abundance		137 mM
10	potassium chloride	natural abundance		2.7 mM
11	H2O	natural abundance		95 %
12	D2O	natural abundance		5 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2KMZ, Strand ID: A Detail

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Release date

2013-06-03

Citation

Structure of the extracellular domains of human and Xenopus Fn14: implications in the evolution of TWEAK and Fn14 interactions
Pellegrini, M., Willen, L., Perroud, M., Krushinskie, D., Strauch, K., Cuervo, H., Day, E.S., Schneider, P., Zheng, T.S.
FEBS J. (2013), 280, 1818-1829, PubMed 23438059 , DOI 10.1111/febs.12206 , Abstract

Related entities 1. hFn14, : 1 : 2 : 2 : 7 entities Detail

Interaction partners 1. hFn14, : 5 interactors Detail

Experiments performed 2 experiments Detail