BMRB: 17241

YAP WW2

Authors

Webb, C., Upadhyay, A., Furutani-Seiki, M., Bagby, S.

Assembly

YAP

Entity

1. YAP (polymer, Thiol state: not present), 46 monomers, 5125.530 Da Detail

GSPNSSPASG PLPEGWEQAI TPEGEIYYIN HKNKTTSWLD PRLETR

Formula weight

5125.53 Da

Source organism

Oryzias latipes

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 90.9 %, Completeness (bb): 98.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.9 % (480 of 528)	85.3 % (237 of 278)	96.6 % (197 of 204)	100.0 % (46 of 46)
Backbone	98.1 % (259 of 264)	97.8 % (88 of 90)	97.8 % (131 of 134)	100.0 % (40 of 40)
Sidechain	85.9 % (263 of 306)	79.3 % (149 of 188)	96.4 % (108 of 112)	100.0 % (6 of 6)
Aromatic	90.9 % (40 of 44)	90.9 % (20 of 22)	90.0 % (18 of 20)	100.0 % (2 of 2)
Methyl	94.4 % (34 of 36)	94.4 % (17 of 18)	94.4 % (17 of 18)

1. entity

GSPNSSPASG PLPEGWEQAI TPEGEIYYIN HKNKTTSWLD PRLETR

Show sample condition

Sample #1

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 273 K, pH 5.5

#	Name	Isotope labeling	Type	Concentration
1	potassium phosphate	natural abundance		50 mM
2	sodium chloride	natural abundance		200 mM
3	EDTA	natural abundance		5 mM
4	YAP WW2 protein	[U-15N]		400.0 ~ 600.0 mM
5	H2O	natural abundance		93 %
6	D2O	natural abundance		7 %

Sample #2

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 273 K, pH 5.5

#	Name	Isotope labeling	Type	Concentration
7	potassium phosphate	natural abundance		50 mM
8	sodium chloride	natural abundance		200 mM
9	EDTA	natural abundance		5 mM
10	YAP WW2 protein	[U-13C; U-15N]		400.0 ~ 600.0 mM
11	H2O	natural abundance		93 %
12	D2O	natural abundance		7 %

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LACS Plot; CA

Referencing offset: -0.12 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.12 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.06 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.22 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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Protein Blocks Logo

Calculated from 21 models in PDB: 2L4J, Strand ID: A Detail

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Release date

2011-03-29

Citation

Structural features and ligand binding properties of tandem WW domains from YAP and TAZ, nuclear effectors of the Hippo pathway
Webb, C., Upadhyay, A., Giuntini, F., Eggleston, I., Furutani-Seiki, M., Ishima, R., Bagby, S.
Biochemistry (2011), 50, 3300-3309, PubMed 21417403 , DOI 10.1021/bi2001888 , Abstract

Related entities 1. YAP, : 1 : 108 entities Detail

Experiments performed 12 experiments Detail