BMRB: 17247

Solution NMR Structure of xenopus Fn14

Authors

Pellegrini, M., Willen, L., Perroud, M., Krushinskie, D., Strauch, K., Cuervo, H., Sun, Y., Day, E.S., Schneider, P., Zheng, T.S.

Assembly

xeFn14

Entity

1. xeFn14 (polymer, Thiol state: all disulfide bound), 66 monomers, 7685.385 Da Detail

SQGECPEGRA YSQDLGKCME CSVCKNSEKS DFCQNCPSKT EQPDFPWIWV EQKLISEEDL HHHHHH

Formula weight

7685.385 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS5:SG	1:CYS18:SG
2	disulfide	sing	1:CYS21:SG	1:CYS36:SG
3	disulfide	sing	1:CYS24:SG	1:CYS33:SG

Source organism

Xenopus laevis

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 65.2 %, Completeness: 51.7 %, Completeness (bb): 62.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	51.7 % (246 of 476)	51.6 % (209 of 405)	52.1 % (37 of 71)
Backbone	62.2 % (120 of 193)	63.4 % (83 of 131)	59.7 % (37 of 62)
Sidechain	44.9 % (127 of 283)	46.4 % (127 of 274)	0.0 % (0 of 9)
Aromatic	30.0 % (12 of 40)	31.6 % (12 of 38)	0.0 % (0 of 2)
Methyl	62.5 % (10 of 16)	62.5 % (10 of 16)

1. xeFn14

SQGECPEGRA YSQDLGKCME CSVCKNSEKS DFCQNCPSKT EQPDFPWIWV EQKLISEEDL HHHHHH

Show sample condition

Sample

Solvent system 95% H2O, 5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
1	xeFn14	[U-99% 15N]		500.0 ~ 700.0 uM
2	sodium phosphate	natural abundance		10 mM
3	sodium chloride	natural abundance		137 mM
4	potassium chloride	natural abundance		2.7 mM
5	H2O	natural abundance		95 %
6	D2O	natural abundance		5 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2KN0, Strand ID: A Detail

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Release date

2013-06-03

Citation

Structure of the extracellular domains of human and Xenopus Fn14: implications in the evolution of TWEAK and Fn14 interactions
Pellegrini, M., Willen, L., Perroud, M., Krushinskie, D., Strauch, K., Cuervo, H., Day, E.S., Schneider, P., Zheng, T.S.
FEBS J. (2013), 280, 1818-1829, PubMed 23438059 , DOI 10.1111/febs.12206 , Abstract

Entries sharing articles BMRB: 2 entries Detail

Related entities 1. xeFn14, : 1 entities Detail

Experiments performed 1 experiments Detail