BMRB: 17252

Solution NMR Structure of BCMA

Authors

Pellegrini, M., Willen, L., Perroud, M., Krushinskie, D., Strauch, K., Cuervo, H., Sun, Y., Day, E.S., Schneider, P., Zheng, T.S.

Assembly

BCMA

Entity

1. BCMA (polymer, Thiol state: all disulfide bound), 50 monomers, 5599.362 Da Detail

LQMAGQCSQN EYFDSLLHAC IPCQLRCSSN TPPLTCQRYC NASVTNSVKX

Formula weight

5599.362 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS7:SG	1:CYS20:SG
2	disulfide	sing	1:CYS23:SG	1:CYS36:SG
3	disulfide	sing	1:CYS27:SG	1:CYS40:SG

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.0 %, Completeness: 56.8 %, Completeness (bb): 89.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	56.8 % (162 of 285)	56.8 % (162 of 285)
Backbone	89.6 % (86 of 96)	89.6 % (86 of 96)
Sidechain	40.2 % (76 of 189)	40.2 % (76 of 189)
Aromatic	0.0 % (0 of 15)	0.0 % (0 of 15)
Methyl	36.4 % (8 of 22)	36.4 % (8 of 22)

1. BCMA

LQMAGQCSQN EYFDSLLHAC IPCQLRCSSN TPPLTCQRYC NASVTNSVKX

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Sample

Solvent system 95% H2O, 5% D2O, Pressure 1 atm, Temperature 308 (±10) K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	BCMA	natural abundance		900 uM
2	sodium phosphate	natural abundance		10 mM
3	sodium azide	natural abundance		0.02 %
4	H2O	natural abundance		95 %
5	D2O	natural abundance		5 %

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Calculated from 20 models in PDB: 2KN1, Strand ID: A Detail

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Release date

2013-06-03

Citation

Structure of the extracellular domains of human and Xenopus Fn14: implications in the evolution of TWEAK and Fn14 interactions
Pellegrini, M., Willen, L., Perroud, M., Krushinskie, D., Strauch, K., Cuervo, H., Day, E.S., Schneider, P., Zheng, T.S.
FEBS J. (2013), 280, 1818-1829, PubMed 23438059 , DOI 10.1111/febs.12206 , Abstract

Related entities 1. BCMA, : 1 : 6 : 5 entities Detail

Interaction partners 1. BCMA, : 2 interactors Detail

Experiments performed 1 experiments Detail