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BMRB: 17255

2L4T

GIP/Glutaminase L peptide complex

Authors

Zoetewey, D.L., Ovee, M., Banerjee, M., Bhaskaran, R., Mohanty, S.

Assembly

GIP/Glutaminase L peptide complex

Entity

1. Glutaminase Interacting Protein 3 (polymer, Thiol state: not present), 124 monomers, 13734.54 Da Detail

MSYIPGQPVT AVVQRVEIHK LRQGENLILG FSIGGGIDQD PSQNPFSEDK TDKGIYVTRV SEGGPAEIAG LQIGDKIMQV NGWDMTMVTH DQARKRLTKR SEEVVRLLVT RQSLQKAVQQ SMLS

2. Glutaminase L peptide (polymer, Thiol state: not present), 8 monomers, 949.0791 Da Detail

KENLESMV

Total weight

14683.619 Da

Max. entity weight

13734.54 Da

Source organism

Exptl. method

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 84.9 %, Completeness (bb): 81.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	84.9 % (1301 of 1532)	95.0 % (764 of 804)	69.3 % (405 of 584)	91.7 % (132 of 144)
Backbone	81.1 % (634 of 782)	97.8 % (265 of 271)	65.4 % (251 of 384)	92.9 % (118 of 127)
Sidechain	89.4 % (778 of 870)	93.6 % (499 of 533)	82.8 % (265 of 320)	82.4 % (14 of 17)
Aromatic	23.2 % (13 of 56)	42.9 % (12 of 28)	0.0 % (0 of 27)	100.0 % (1 of 1)
Methyl	96.7 % (147 of 152)	100.0 % (76 of 76)	93.4 % (71 of 76)

1. Glutaminase Interacting Protein 3

MSYIPGQPVT AVVQRVEIHK LRQGENLILG FSIGGGIDQD PSQNPFSEDK TDKGIYVTRV SEGGPAEIAG LQIGDKIMQV NGWDMTMVTH DQARKRLTKR SEEVVRLLVT RQSLQKAVQQ SMLS

2. Glutaminase L peptide

KENLESMV

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	Glutaminase Interacting Protein 3	[U-99% 13C; U-99% 15N]		0.5-1 mM
2	Glutaminase L peptide	natural abundance		1-3 mM
3	sodium phosphate	natural abundance		50 mM
4	sodium azide	natural abundance		0.1 % w/v
5	EDTA	natural abundance		1 mM
6	H2O	natural abundance		95 %
7	D2O	natural abundance		5 %

Protein Blocks Logo

Calculated from 20 models in PDB: 2L4T, Strand ID: A, B Detail

Release date

2011-03-23

Citation

Promiscuous binding at the crossroads of numerous cancer pathways: insight from the binding of glutaminase interacting protein with glutaminase L
Zoetewey, D.L., Ovee, M., Banerjee, M., Bhaskaran, R., Mohanty, S.
Biochemistry (2011), 50, 3528-3539, PubMed 21417405 , DOI 10.1021/bi102055y , Abstract

Related entities 1. Glutaminase Interacting Protein 3, : 1 : 1 : 40 entities Detail

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	Glutaminase Interacting Protein 3	[U-99% 13C; U-99% 15N]		0.5-1 mM
2	Glutaminase L peptide	natural abundance		1-3 mM
3	sodium phosphate	natural abundance		50 mM
4	sodium azide	natural abundance		0.1 % w/v
5	EDTA	natural abundance		1 mM
6	H2O	natural abundance		95 %
7	D2O	natural abundance		5 %

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	Glutaminase Interacting Protein 3	[U-99% 13C; U-99% 15N]		0.5-1 mM
2	Glutaminase L peptide	natural abundance		1-3 mM
3	sodium phosphate	natural abundance		50 mM
4	sodium azide	natural abundance		0.1 % w/v
5	EDTA	natural abundance		1 mM
6	H2O	natural abundance		95 %
7	D2O	natural abundance		5 %

3 3D HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	Glutaminase Interacting Protein 3	[U-99% 13C; U-99% 15N]		0.5-1 mM
2	Glutaminase L peptide	natural abundance		1-3 mM
3	sodium phosphate	natural abundance		50 mM
4	sodium azide	natural abundance		0.1 % w/v
5	EDTA	natural abundance		1 mM
6	H2O	natural abundance		95 %
7	D2O	natural abundance		5 %

4 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	Glutaminase Interacting Protein 3	[U-99% 13C; U-99% 15N]		0.5-1 mM
2	Glutaminase L peptide	natural abundance		1-3 mM
3	sodium phosphate	natural abundance		50 mM
4	sodium azide	natural abundance		0.1 % w/v
5	EDTA	natural abundance		1 mM
6	H2O	natural abundance		95 %
7	D2O	natural abundance		5 %

5 3D C(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	Glutaminase Interacting Protein 3	[U-99% 13C; U-99% 15N]		0.5-1 mM
2	Glutaminase L peptide	natural abundance		1-3 mM
3	sodium phosphate	natural abundance		50 mM
4	sodium azide	natural abundance		0.1 % w/v
5	EDTA	natural abundance		1 mM
6	H2O	natural abundance		95 %
7	D2O	natural abundance		5 %

6 3D H(CCO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	Glutaminase Interacting Protein 3	[U-99% 13C; U-99% 15N]		0.5-1 mM
2	Glutaminase L peptide	natural abundance		1-3 mM
3	sodium phosphate	natural abundance		50 mM
4	sodium azide	natural abundance		0.1 % w/v
5	EDTA	natural abundance		1 mM
6	H2O	natural abundance		95 %
7	D2O	natural abundance		5 %

7 3D 1H-15N TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	Glutaminase Interacting Protein 3	[U-99% 13C; U-99% 15N]		0.5-1 mM
2	Glutaminase L peptide	natural abundance		1-3 mM
3	sodium phosphate	natural abundance		50 mM
4	sodium azide	natural abundance		0.1 % w/v
5	EDTA	natural abundance		1 mM
6	H2O	natural abundance		95 %
7	D2O	natural abundance		5 %

8 3D HCCH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	Glutaminase Interacting Protein 3	[U-99% 13C; U-99% 15N]		0.5-1 mM
2	Glutaminase L peptide	natural abundance		1-3 mM
3	sodium phosphate	natural abundance		50 mM
4	sodium azide	natural abundance		0.1 % w/v
5	EDTA	natural abundance		1 mM
6	H2O	natural abundance		95 %
7	D2O	natural abundance		5 %

9 3D 1H-15N NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	Glutaminase Interacting Protein 3	[U-99% 13C; U-99% 15N]		0.5-1 mM
2	Glutaminase L peptide	natural abundance		1-3 mM
3	sodium phosphate	natural abundance		50 mM
4	sodium azide	natural abundance		0.1 % w/v
5	EDTA	natural abundance		1 mM
6	H2O	natural abundance		95 %
7	D2O	natural abundance		5 %

10 3D 1H-13C NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	Glutaminase Interacting Protein 3	[U-99% 13C; U-99% 15N]		0.5-1 mM
2	Glutaminase L peptide	natural abundance		1-3 mM
3	sodium phosphate	natural abundance		50 mM
4	sodium azide	natural abundance		0.1 % w/v
5	EDTA	natural abundance		1 mM
6	H2O	natural abundance		95 %
7	D2O	natural abundance		5 %

11 3D 1H-13C FILTERED NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	Glutaminase Interacting Protein 3	[U-99% 13C; U-99% 15N]		0.5-1 mM
2	Glutaminase L peptide	natural abundance		1-3 mM
3	sodium phosphate	natural abundance		50 mM
4	sodium azide	natural abundance		0.1 % w/v
5	EDTA	natural abundance		1 mM
6	H2O	natural abundance		95 %
7	D2O	natural abundance		5 %

12 3D 1H-15N FILTERED NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	Glutaminase Interacting Protein 3	[U-99% 13C; U-99% 15N]		0.5-1 mM
2	Glutaminase L peptide	natural abundance		1-3 mM
3	sodium phosphate	natural abundance		50 mM
4	sodium azide	natural abundance		0.1 % w/v
5	EDTA	natural abundance		1 mM
6	H2O	natural abundance		95 %
7	D2O	natural abundance		5 %

nullKeywords GIP, Glutaminase L, NMR, PDZ domain, protein/peptide complex