BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	97.1 % (1292 of 1331)	97.1 % (660 of 680)	97.0 % (512 of 528)	97.6 % (120 of 123)
Backbone	96.9 % (680 of 702)	97.1 % (231 of 238)	96.9 % (342 of 353)	96.4 % (107 of 111)
Sidechain	97.2 % (721 of 742)	96.8 % (428 of 442)	97.6 % (281 of 288)	100.0 % (12 of 12)
Aromatic	94.2 % (98 of 104)	94.2 % (49 of 52)	94.0 % (47 of 50)	100.0 % (2 of 2)
Methyl	98.6 % (138 of 140)	98.6 % (69 of 70)	98.6 % (69 of 70)

1. VirB7-Xac2622

GSHMTKPAPD FGGRWKHVNH FDEAPTEIPL YTSYTYQATP MDGTLKTMLE RWAADSNMQL SYNLPSDYTL IGPVSAISTT SVQQAATELS AVYAAQGVSV SVSANKLLVQ PVPVSSGAKL

Show sample condition

Sample #1

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0, Details 10mM deuterated sodium acetate buffer pH 5.0 50mM NaCl

#	Name	Isotope labeling	Concentration
1	VirB7 - Xac2622	[U-99% 13C; U-99% 15N]	0.2 mM
2	sodium acetate	[U-99% 2H]	10 mM
3	sodium chloride	natural abundance	50 mM
4	H2O	natural abundance	93 %
5	D2O	natural abundance	7 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0, Details 10mM deuterated sodium acetate buffer pH 5.0 50mM NaCl

#	Name	Isotope labeling	Concentration
6	VirB7 - Xac2622	[U-99% 13C; U-99% 15N]	0.2 mM
7	sodium acetate	[U-99% 2H]	10 mM
8	sodium chloride	natural abundance	50 mM
9	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	1.0 ppm	na	indirect	0.2514495
¹H	water	protons	4.63 ppm	internal	direct	1.0
¹⁵N	water	protons	1.0 ppm	na	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	1.0 ppm	na	indirect	0.2514495
¹H	water	protons	4.63 ppm	internal	direct	1.0
¹⁵N	water	protons	1.0 ppm	na	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	1.0 ppm	na	indirect	0.2514495
¹H	water	protons	4.63 ppm	internal	direct	1.0
¹⁵N	water	protons	1.0 ppm	na	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	1.0 ppm	na	indirect	0.2514495
¹H	water	protons	4.63 ppm	internal	direct	1.0
¹⁵N	water	protons	1.0 ppm	na	indirect	0.1013291

Referencing offset: 0.37 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L4W, Strand ID: A Detail

Release date

2011-05-18

Citation

A component of the Xanthomonadaceae type IV secretion system combines a VirB7 motif with a N0 domain found in outer membrane transport proteins
Souza, D.P., Andrade, M.O., Alvarez-Martinez, C.E., Arantes, G.M., Farah, C.S., Salinas, R.K.
PLoS Pathog. (2011), 7, e1002031-e1002031, PubMed 21589901 , DOI 10.1371/journal.ppat.1002031 , Abstract

Related entities 1. VirB7-Xac2622 monomer, : 1 : 3 entities Detail

Experiments performed 20 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz Varian Unity INOVA 600 MHz with Cold probe at The Brazilian Synchrotron Light Laboratory (LNLS), Campinas - Brazil

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0, Details 10mM deuterated sodium acetate buffer pH 5.0 50mM NaCl

#	Name	Isotope labeling	Concentration
1	VirB7 - Xac2622	[U-99% 13C; U-99% 15N]	0.2 mM
2	sodium acetate	[U-99% 2H]	10 mM
3	sodium chloride	natural abundance	50 mM
4	H2O	natural abundance	93 %
5	D2O	natural abundance	7 %

2 2D 1H-13C HSQC

Instrument

Varian INOVA - 600 MHz Varian Unity INOVA 600 MHz with Cold probe at The Brazilian Synchrotron Light Laboratory (LNLS), Campinas - Brazil

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0, Details 10mM deuterated sodium acetate buffer pH 5.0 50mM NaCl

#	Name	Isotope labeling	Concentration
6	VirB7 - Xac2622	[U-99% 13C; U-99% 15N]	0.2 mM
7	sodium acetate	[U-99% 2H]	10 mM
8	sodium chloride	natural abundance	50 mM
9	D2O	natural abundance	100 %

3 2D 1H-1H TOCSY

Instrument

Bruker DRX - 500 MHz Bruker DRX 500 MHZ, Institute of Chemistry, University of Sao Paulo, Sao Paulo - Brazil

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0, Details 10mM deuterated sodium acetate buffer pH 5.0 50mM NaCl

#	Name	Isotope labeling	Concentration
6	VirB7 - Xac2622	[U-99% 13C; U-99% 15N]	0.2 mM
7	sodium acetate	[U-99% 2H]	10 mM
8	sodium chloride	natural abundance	50 mM
9	D2O	natural abundance	100 %

4 2D 1H-1H NOESY

Instrument

Varian INOVA - 600 MHz Varian Unity INOVA 600 MHz with Cold probe at The Brazilian Synchrotron Light Laboratory (LNLS), Campinas - Brazil

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0, Details 10mM deuterated sodium acetate buffer pH 5.0 50mM NaCl

#	Name	Isotope labeling	Concentration
1	VirB7 - Xac2622	[U-99% 13C; U-99% 15N]	0.2 mM
2	sodium acetate	[U-99% 2H]	10 mM
3	sodium chloride	natural abundance	50 mM
4	H2O	natural abundance	93 %
5	D2O	natural abundance	7 %

5 3D HNCO

Instrument

Bruker DRX - 500 MHz Bruker DRX 500 MHZ, Institute of Chemistry, University of Sao Paulo, Sao Paulo - Brazil

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0, Details 10mM deuterated sodium acetate buffer pH 5.0 50mM NaCl

#	Name	Isotope labeling	Concentration
1	VirB7 - Xac2622	[U-99% 13C; U-99% 15N]	0.2 mM
2	sodium acetate	[U-99% 2H]	10 mM
3	sodium chloride	natural abundance	50 mM
4	H2O	natural abundance	93 %
5	D2O	natural abundance	7 %

6 3D HN(CA)CO

Instrument

Varian INOVA - 600 MHz Varian Unity INOVA 600 MHz with Cold probe at The Brazilian Synchrotron Light Laboratory (LNLS), Campinas - Brazil

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0, Details 10mM deuterated sodium acetate buffer pH 5.0 50mM NaCl

#	Name	Isotope labeling	Concentration
1	VirB7 - Xac2622	[U-99% 13C; U-99% 15N]	0.2 mM
2	sodium acetate	[U-99% 2H]	10 mM
3	sodium chloride	natural abundance	50 mM
4	H2O	natural abundance	93 %
5	D2O	natural abundance	7 %

7 3D HNCA

Instrument

Bruker DRX - 500 MHz Bruker DRX 500 MHZ, Institute of Chemistry, University of Sao Paulo, Sao Paulo - Brazil

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0, Details 10mM deuterated sodium acetate buffer pH 5.0 50mM NaCl

#	Name	Isotope labeling	Concentration
1	VirB7 - Xac2622	[U-99% 13C; U-99% 15N]	0.2 mM
2	sodium acetate	[U-99% 2H]	10 mM
3	sodium chloride	natural abundance	50 mM
4	H2O	natural abundance	93 %
5	D2O	natural abundance	7 %

8 3D HN(CO)CA

Instrument

Bruker DRX - 500 MHz Bruker DRX 500 MHZ, Institute of Chemistry, University of Sao Paulo, Sao Paulo - Brazil

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0, Details 10mM deuterated sodium acetate buffer pH 5.0 50mM NaCl

#	Name	Isotope labeling	Concentration
1	VirB7 - Xac2622	[U-99% 13C; U-99% 15N]	0.2 mM
2	sodium acetate	[U-99% 2H]	10 mM
3	sodium chloride	natural abundance	50 mM
4	H2O	natural abundance	93 %
5	D2O	natural abundance	7 %

9 3D HNCACB

Instrument

Varian INOVA - 600 MHz Varian Unity INOVA 600 MHz with Cold probe at The Brazilian Synchrotron Light Laboratory (LNLS), Campinas - Brazil

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0, Details 10mM deuterated sodium acetate buffer pH 5.0 50mM NaCl

#	Name	Isotope labeling	Concentration
1	VirB7 - Xac2622	[U-99% 13C; U-99% 15N]	0.2 mM
2	sodium acetate	[U-99% 2H]	10 mM
3	sodium chloride	natural abundance	50 mM
4	H2O	natural abundance	93 %
5	D2O	natural abundance	7 %

10 3D CBCA(CO)NH

Instrument

Varian INOVA - 600 MHz Varian Unity INOVA 600 MHz with Cold probe at The Brazilian Synchrotron Light Laboratory (LNLS), Campinas - Brazil

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0, Details 10mM deuterated sodium acetate buffer pH 5.0 50mM NaCl

#	Name	Isotope labeling	Concentration
1	VirB7 - Xac2622	[U-99% 13C; U-99% 15N]	0.2 mM
2	sodium acetate	[U-99% 2H]	10 mM
3	sodium chloride	natural abundance	50 mM
4	H2O	natural abundance	93 %
5	D2O	natural abundance	7 %

11 2D 1H-1H NOESY

Instrument

Bruker DRX - 500 MHz Bruker DRX 500 MHZ, Institute of Chemistry, University of Sao Paulo, Sao Paulo - Brazil

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0, Details 10mM deuterated sodium acetate buffer pH 5.0 50mM NaCl

#	Name	Isotope labeling	Concentration
6	VirB7 - Xac2622	[U-99% 13C; U-99% 15N]	0.2 mM
7	sodium acetate	[U-99% 2H]	10 mM
8	sodium chloride	natural abundance	50 mM
9	D2O	natural abundance	100 %

12 3D C(CO)NH

Instrument

Varian INOVA - 600 MHz Varian Unity INOVA 600 MHz with Cold probe at The Brazilian Synchrotron Light Laboratory (LNLS), Campinas - Brazil

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0, Details 10mM deuterated sodium acetate buffer pH 5.0 50mM NaCl

#	Name	Isotope labeling	Concentration
1	VirB7 - Xac2622	[U-99% 13C; U-99% 15N]	0.2 mM
2	sodium acetate	[U-99% 2H]	10 mM
3	sodium chloride	natural abundance	50 mM
4	H2O	natural abundance	93 %
5	D2O	natural abundance	7 %

13 3D HBHA(CO)NH

Instrument

Varian INOVA - 600 MHz Varian Unity INOVA 600 MHz with Cold probe at The Brazilian Synchrotron Light Laboratory (LNLS), Campinas - Brazil

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0, Details 10mM deuterated sodium acetate buffer pH 5.0 50mM NaCl

#	Name	Isotope labeling	Concentration
1	VirB7 - Xac2622	[U-99% 13C; U-99% 15N]	0.2 mM
2	sodium acetate	[U-99% 2H]	10 mM
3	sodium chloride	natural abundance	50 mM
4	H2O	natural abundance	93 %
5	D2O	natural abundance	7 %

14 3D H(CCO)NH

Instrument

Varian INOVA - 600 MHz Varian Unity INOVA 600 MHz with Cold probe at The Brazilian Synchrotron Light Laboratory (LNLS), Campinas - Brazil

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0, Details 10mM deuterated sodium acetate buffer pH 5.0 50mM NaCl

#	Name	Isotope labeling	Concentration
1	VirB7 - Xac2622	[U-99% 13C; U-99% 15N]	0.2 mM
2	sodium acetate	[U-99% 2H]	10 mM
3	sodium chloride	natural abundance	50 mM
4	H2O	natural abundance	93 %
5	D2O	natural abundance	7 %

15 3D HCCH-TOCSY

Instrument

Varian INOVA - 600 MHz Varian Unity INOVA 600 MHz with Cold probe at The Brazilian Synchrotron Light Laboratory (LNLS), Campinas - Brazil

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0, Details 10mM deuterated sodium acetate buffer pH 5.0 50mM NaCl

#	Name	Isotope labeling	Concentration
6	VirB7 - Xac2622	[U-99% 13C; U-99% 15N]	0.2 mM
7	sodium acetate	[U-99% 2H]	10 mM
8	sodium chloride	natural abundance	50 mM
9	D2O	natural abundance	100 %

16 3D HNHA

Instrument

Varian INOVA - 600 MHz Varian Unity INOVA 600 MHz with Cold probe at The Brazilian Synchrotron Light Laboratory (LNLS), Campinas - Brazil

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0, Details 10mM deuterated sodium acetate buffer pH 5.0 50mM NaCl

#	Name	Isotope labeling	Concentration
1	VirB7 - Xac2622	[U-99% 13C; U-99% 15N]	0.2 mM
2	sodium acetate	[U-99% 2H]	10 mM
3	sodium chloride	natural abundance	50 mM
4	H2O	natural abundance	93 %
5	D2O	natural abundance	7 %

17 3D 1H-15N NOESY

Instrument

Varian INOVA - 600 MHz Varian Unity INOVA 600 MHz with Cold probe at The Brazilian Synchrotron Light Laboratory (LNLS), Campinas - Brazil

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0, Details 10mM deuterated sodium acetate buffer pH 5.0 50mM NaCl

#	Name	Isotope labeling	Concentration
1	VirB7 - Xac2622	[U-99% 13C; U-99% 15N]	0.2 mM
2	sodium acetate	[U-99% 2H]	10 mM
3	sodium chloride	natural abundance	50 mM
4	H2O	natural abundance	93 %
5	D2O	natural abundance	7 %

18 3D 1H-15N TOCSY

Instrument

Varian INOVA - 600 MHz Varian Unity INOVA 600 MHz with Cold probe at The Brazilian Synchrotron Light Laboratory (LNLS), Campinas - Brazil

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0, Details 10mM deuterated sodium acetate buffer pH 5.0 50mM NaCl

#	Name	Isotope labeling	Concentration
1	VirB7 - Xac2622	[U-99% 13C; U-99% 15N]	0.2 mM
2	sodium acetate	[U-99% 2H]	10 mM
3	sodium chloride	natural abundance	50 mM
4	H2O	natural abundance	93 %
5	D2O	natural abundance	7 %

19 3D 1H-13C NOESY

Instrument

Varian INOVA - 600 MHz Varian Unity INOVA 600 MHz with Cold probe at The Brazilian Synchrotron Light Laboratory (LNLS), Campinas - Brazil

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0, Details 10mM deuterated sodium acetate buffer pH 5.0 50mM NaCl

#	Name	Isotope labeling	Concentration
6	VirB7 - Xac2622	[U-99% 13C; U-99% 15N]	0.2 mM
7	sodium acetate	[U-99% 2H]	10 mM
8	sodium chloride	natural abundance	50 mM
9	D2O	natural abundance	100 %

20 2D 1H-15N HSQC J-modulated

Instrument

Bruker DRX - 500 MHz Bruker DRX 500 MHZ, Institute of Chemistry, University of Sao Paulo, Sao Paulo - Brazil

Sample

State anisotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 303 K, pH 5.0, Details 10mM deuterated sodium acetate buffer pH 5.0 50mM NaCl

#	Name	Isotope labeling	Concentration
1	VirB7 - Xac2622	[U-99% 13C; U-99% 15N]	0.2 mM
2	sodium acetate	[U-99% 2H]	10 mM
3	sodium chloride	natural abundance	50 mM
4	H2O	natural abundance	93 %
5	D2O	natural abundance	7 %

nullKeywords NMR, T4SS, VirB7, Xanthomonas, X-ray crystallography, N0 domain, NMR structure

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Found 1 Document (0.833 seconds)

BMRB: 17257

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. VirB7-Xac2622 monomer, : 1 : 3 entities Detail

Experiments performed 20 experiments Detail

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