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BMRB: 17260

2LEN

Backbone and side-chain 1H, 13C and 15N assignments of the UCHL1 S18Y variant

Authors

Tse, H., Sze, K.

Assembly

UCHL1 S18Y variant

Entity

1. UCHL1 S18Y variant (polymer, Thiol state: unknown), 231 monomers, 25965.25 Da Detail

MQLKPMEINP EMLNKVLYRL GVAGQWRFVD VLGLEEESLG SVPAPACALL LLFPLTAQHE NFRKKQIEEL KGQEVSPKVY FMKQTIGNSC GTIGLIHAVA NNQDKLGFED GSVLKQFLSE TEKMSPEDRA KCFEKNEAIQ AAHDAVAQEG QCRVDDKVNF HFILFNNVDG HLYELDGRMP FPVNHGASSE DTLLKDAAKV CREFTEREQG EVRFSAVALC KAALEHHHHH H

Formula weight

25965.25 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.6 %, Completeness: 83.3 %, Completeness (bb): 96.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	83.3 % (2251 of 2702)	81.7 % (1151 of 1408)	82.4 % (864 of 1048)	95.9 % (236 of 246)
Backbone	96.8 % (1324 of 1368)	94.9 % (444 of 468)	97.8 % (663 of 678)	97.7 % (217 of 222)
Sidechain	73.3 % (1136 of 1550)	75.3 % (708 of 940)	69.8 % (409 of 586)	79.2 % (19 of 24)
Aromatic	6.5 % (14 of 214)	7.5 % (8 of 107)	4.7 % (5 of 106)	100.0 % (1 of 1)
Methyl	91.4 % (223 of 244)	94.3 % (115 of 122)	88.5 % (108 of 122)

1. UCHL1 S18Y variant

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	UCHL1 S18Y variant	[U-99% 13C; U-99% 15N]	0.7 ~ 0.8 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	DTT	natural abundance	3 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 4 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 5 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LEN, Strand ID: A Detail

Release date

2011-02-09

Citation

Backbone and side-chain 1H, 15N and 13C resonance assignments of S18Y mutant of ubiquitin carboxy-terminal hydrolase L1
Tse, H., Hu, H., Sze, K.
Biomol. NMR Assign. (2011), 5, 165-168, PubMed 21298373 , DOI 10.1007/s12104-011-9292-7 , Abstract

Related entities 1. UCHL1 S18Y variant, : 1 : 11 : 102 entities Detail

Interaction partners 1. UCHL1 S18Y variant, : 33 interactors Detail

More details for 33 interactors identified by 15 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC