BMRBj - BMREntryMaster

Found 1 Document (0.814 seconds)

BMRB: 17263

2L52

Solution structure of the small archaeal modifier protein 1 (SAMP1) from Methanosarcina acetivorans.

Authors

Damberger, F.F., Ranjan, N., Sutter, M., Allain, F., Weber-Ban, E.

Assembly

Small archaeal modifier protein 1 from Methanosarcina acetivorans

Entity

1. Small archaeal modifier protein 1 from Methanosarcina acetivorans (polymer, Thiol state: all free), 99 monomers, 10696.30 Da Detail

GHMAEVKVKL FANLREAAGT PELPLSGEKV IDVLLSLTDK YPALKYVIFE KGDEKSEILI LCGSINILIN GNNIRHLEGL ETLLKDSDEI GILPPVSGG

Formula weight

10696.3 Da

Source organism

Methanosarcina acetivorans

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, simulated annealing

Data set

H_exch_protection_factors, H_exch_rates, assigned_chemical_shifts, heteronucl_NOEs

Chem. Shift Complete

Sequence coverage: 99.0 %, Completeness: 98.4 %, Completeness (bb): 97.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	98.4 % (1126 of 1144)	98.5 % (584 of 593)	98.2 % (444 of 452)	99.0 % (98 of 99)
Backbone	97.4 % (569 of 584)	97.0 % (197 of 203)	97.2 % (279 of 287)	98.9 % (93 of 94)
Sidechain	99.2 % (644 of 649)	99.0 % (386 of 390)	99.6 % (253 of 254)	100.0 % (5 of 5)
Aromatic	100.0 % (44 of 44)	100.0 % (22 of 22)	100.0 % (22 of 22)
Methyl	98.6 % (142 of 144)	98.6 % (71 of 72)	98.6 % (71 of 72)

1. Small archaeal modifier protein 1 from Methanosarcina acetivorans

GHMAEVKVKL FANLREAAGT PELPLSGEKV IDVLLSLTDK YPALKYVIFE KGDEKSEILI LCGSINILIN GNNIRHLEGL ETLLKDSDEI GILPPVSGG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	SAMP1	[U-99% 13C; U-99% 15N]	1 mM
2	sodium chloride	natural abundance	150 mM
3	sodium phosphate	natural abundance	20 mM
4	EDTA	natural abundance	0.1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
7	SAMP1	[U-99% 15N]	1 mM
8	sodium chloride	natural abundance	150 mM
9	sodium phosphate	natural abundance	20 mM
10	EDTA	natural abundance	0.1 mM
11	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.24 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L52, Strand ID: A Detail

Heteronucl. NOE

76 NOE values in 1 lists
Value type peak height, Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.8 (±0.1) Detail

¹H exch. protect.

41 values in 1 lists
Calculation method entry_citation, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.8 (±0.1) Detail

¹H exchange rate

41 k_ex values in 1 lists
Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.8 (±0.1) Detail

Release date

2015-11-02

Citation

Solution structure and activation mechanism of ubiquitin-like small archaeal modifier proteins
Ranjan, N., Damberger, F.F., Sutter, M., Allain, F., Weber-Ban, E.
J. Mol. Biol. (2011), 405, 1040-1055, PubMed 21112336 , DOI 10.1016/j.jmb.2010.11.040 , Abstract

Related entities 1. Small archaeal modifier protein 1 from Methanosarcina acetivorans, : 1 : 37 entities Detail

Experiments performed 12 experiments Detail

1 3D 1H-15N NOESY

Instrument

Bruker Avance - 900 MHz equipped with a triple-channel probehead and actively shielded gradients.

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	SAMP1	[U-99% 13C; U-99% 15N]	1 mM
2	sodium chloride	natural abundance	150 mM
3	sodium phosphate	natural abundance	20 mM
4	EDTA	natural abundance	0.1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

2 3D 1H-13C NOESY

Instrument

Bruker Avance - 900 MHz equipped with a triple-channel probehead and actively shielded gradients.

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	SAMP1	[U-99% 13C; U-99% 15N]	1 mM
2	sodium chloride	natural abundance	150 mM
3	sodium phosphate	natural abundance	20 mM
4	EDTA	natural abundance	0.1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

3 3D 1H-13C NOESY

Instrument

Bruker Avance - 900 MHz equipped with a triple-channel probehead and actively shielded gradients.

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	SAMP1	[U-99% 13C; U-99% 15N]	1 mM
2	sodium chloride	natural abundance	150 mM
3	sodium phosphate	natural abundance	20 mM
4	EDTA	natural abundance	0.1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

4 4D APSY-HACANH

Instrument

Bruker Avance - 700 MHz equipped with a triple-channel probehead and actively shielded gradients.

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	SAMP1	[U-99% 13C; U-99% 15N]	1 mM
2	sodium chloride	natural abundance	150 mM
3	sodium phosphate	natural abundance	20 mM
4	EDTA	natural abundance	0.1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

5 5D APSY-CBCACONH

Instrument

Bruker Avance - 700 MHz equipped with a triple-channel probehead and actively shielded gradients.

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	SAMP1	[U-99% 13C; U-99% 15N]	1 mM
2	sodium chloride	natural abundance	150 mM
3	sodium phosphate	natural abundance	20 mM
4	EDTA	natural abundance	0.1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

6 5D APSY-HACACONH

Instrument

Bruker Avance - 700 MHz equipped with a triple-channel probehead and actively shielded gradients.

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	SAMP1	[U-99% 13C; U-99% 15N]	1 mM
2	sodium chloride	natural abundance	150 mM
3	sodium phosphate	natural abundance	20 mM
4	EDTA	natural abundance	0.1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

7 5D APSY-HC(CC-TOCSY)CONH

Instrument

Bruker Avance - 700 MHz equipped with a triple-channel probehead and actively shielded gradients.

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	SAMP1	[U-99% 13C; U-99% 15N]	1 mM
2	sodium chloride	natural abundance	150 mM
3	sodium phosphate	natural abundance	20 mM
4	EDTA	natural abundance	0.1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

8 4D APSY-HCCH-COSY

Instrument

Bruker Avance - 700 MHz equipped with a triple-channel probehead and actively shielded gradients.

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	SAMP1	[U-99% 13C; U-99% 15N]	1 mM
2	sodium chloride	natural abundance	150 mM
3	sodium phosphate	natural abundance	20 mM
4	EDTA	natural abundance	0.1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

9 4D aromatic APSY-HCCH-COSY

Instrument

Bruker Avance - 700 MHz equipped with a triple-channel probehead and actively shielded gradients.

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	SAMP1	[U-99% 13C; U-99% 15N]	1 mM
2	sodium chloride	natural abundance	150 mM
3	sodium phosphate	natural abundance	20 mM
4	EDTA	natural abundance	0.1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

10 4D APSY-HBCB(CG)CDHD

Instrument

Bruker Avance - 700 MHz equipped with a triple-channel probehead and actively shielded gradients.

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	SAMP1	[U-99% 13C; U-99% 15N]	1 mM
2	sodium chloride	natural abundance	150 mM
3	sodium phosphate	natural abundance	20 mM
4	EDTA	natural abundance	0.1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

11 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz equipped with a triple-channel probehead and actively shielded gradients.

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
7	SAMP1	[U-99% 15N]	1 mM
8	sodium chloride	natural abundance	150 mM
9	sodium phosphate	natural abundance	20 mM
10	EDTA	natural abundance	0.1 mM
11	D2O	natural abundance	100 %

12 2D 15N{1H}-NOE-[15N,1H]-HSQC

Instrument

Bruker Avance - 500 MHz equipped with a triple-channel probehead and actively shielded gradients.

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	SAMP1	[U-99% 13C; U-99% 15N]	1 mM
2	sodium chloride	natural abundance	150 mM
3	sodium phosphate	natural abundance	20 mM
4	EDTA	natural abundance	0.1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

null

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (0.814 seconds)

BMRB: 17263

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Small archaeal modifier protein 1 from Methanosarcina acetivorans, : 1 : 37 entities Detail

Experiments performed 12 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample