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Found 1 Document (0.858 seconds)

BMRB: 17265

2L4Z

NMR structure of fusion of CtIP (641-685) to LMO4-LIM1 (18-82)

Authors

Liew, C., Stokes, P.H., Kwan, A.H., Matthews, J.M.

Assembly

Fusion protein

Entity

1. Fusion protein (polymer, Thiol state: all other bound), 123 monomers, 13205.68 Da Detail

GSSLQNNQDV SFENIQWSID PGADLSQYKM DVTVIDTKDG SQSKLGGGGS GGHMGSGGLS WKRCAGCGGK IADRFLLYAM DSYWHSRCLK CSSCQAQLGD IGTSSYTKSG MILCRNDYIR LFG

2. ZN (non-polymer, Thiol state: not present), 65.409 × 2 Da

Total weight

13336.498 Da

Max. entity weight

13205.68 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.1 %, Completeness: 89.8 %, Completeness (bb): 92.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.8 % (1211 of 1349)	93.7 % (655 of 699)	84.2 % (433 of 514)	90.4 % (123 of 136)
Backbone	92.5 % (681 of 736)	91.7 % (242 of 264)	92.6 % (324 of 350)	94.3 % (115 of 122)
Sidechain	88.1 % (632 of 717)	94.9 % (413 of 435)	78.7 % (211 of 268)	57.1 % (8 of 14)
Aromatic	50.9 % (58 of 114)	93.0 % (53 of 57)	7.4 % (4 of 54)	33.3 % (1 of 3)
Methyl	95.9 % (94 of 98)	95.9 % (47 of 49)	95.9 % (47 of 49)

1. Fusion protein

GSSLQNNQDV SFENIQWSID PGADLSQYKM DVTVIDTKDG SQSKLGGGGS GGHMGSGGLS WKRCAGCGGK IADRFLLYAM DSYWHSRCLK CSSCQAQLGD IGTSSYTKSG MILCRNDYIR LFG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 291 K, pH 6 (±0.5)

#	Name	Isotope labeling	Concentration
1	Fusion protein	[U-15N]	150 uM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	150 mM
4	DTT	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 291 K, pH 6 (±0.5)

#	Name	Isotope labeling	Concentration
7	Fusion protein	[U-99% 13C; U-99% 15N]	500 uM
8	sodium phosphate	natural abundance	20 mM
9	sodium chloride	natural abundance	150 mM
10	DTT	natural abundance	1 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 291 K, pH 6 (±0.5)

#	Name	Isotope labeling	Concentration
13	Fusion protein	[U-99% 13C; U-99% 15N]	300 uM
14	sodium phosphate	natural abundance	20 mM
15	sodium chloride	natural abundance	150 mM
16	DTT	natural abundance	1 mM
17	D2O	natural abundance	10 %

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 291 K, pH 6 (±0.5)

#	Name	Isotope labeling	Concentration
18	Fusion protein	natural abundance	500 uM
19	sodium phosphate	natural abundance	20 mM
20	sodium chloride	natural abundance	150 mM
21	DTT	natural abundance	1 mM
22	H2O	natural abundance	90 %
23	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -1.7 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L4Z, Strand ID: A Detail

Release date

2011-06-06

Citation

¹H, ¹⁵N and ¹³C assignments of an intramolecular LMO4-LIM1/CtIP complex
Liew, C., Kwan, A.H., Stokes, P.H., Mackay, J.P., Matthews, J.M.
Biomol. NMR Assign. (2012), 6, 31-34, PubMed 21643835 , DOI 10.1007/s12104-011-9319-0 , Abstract

Related entities 1. Fusion protein, : 1 : 1 entities Detail

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker DRX - 600 MHz TCI Cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 291 K, pH 6 (±0.5)

#	Name	Isotope labeling	Concentration
1	Fusion protein	[U-15N]	150 uM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	150 mM
4	DTT	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

2 2D 1H-13C HSQC

Instrument

Bruker DRX - 600 MHz TCI Cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 291 K, pH 6 (±0.5)

#	Name	Isotope labeling	Concentration
7	Fusion protein	[U-99% 13C; U-99% 15N]	500 uM
8	sodium phosphate	natural abundance	20 mM
9	sodium chloride	natural abundance	150 mM
10	DTT	natural abundance	1 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

3 3D CBCA(CO)NH

Instrument

Bruker DRX - 600 MHz TCI Cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 291 K, pH 6 (±0.5)

#	Name	Isotope labeling	Concentration
7	Fusion protein	[U-99% 13C; U-99% 15N]	500 uM
8	sodium phosphate	natural abundance	20 mM
9	sodium chloride	natural abundance	150 mM
10	DTT	natural abundance	1 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

4 3D HNCACB

Instrument

Bruker DRX - 600 MHz TCI Cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 291 K, pH 6 (±0.5)

#	Name	Isotope labeling	Concentration
7	Fusion protein	[U-99% 13C; U-99% 15N]	500 uM
8	sodium phosphate	natural abundance	20 mM
9	sodium chloride	natural abundance	150 mM
10	DTT	natural abundance	1 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

5 3D HBHA(CO)NH

Instrument

Bruker DRX - 600 MHz TCI Cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 291 K, pH 6 (±0.5)

#	Name	Isotope labeling	Concentration
7	Fusion protein	[U-99% 13C; U-99% 15N]	500 uM
8	sodium phosphate	natural abundance	20 mM
9	sodium chloride	natural abundance	150 mM
10	DTT	natural abundance	1 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

6 3D HNCO

Instrument

Bruker DRX - 600 MHz TCI Cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 291 K, pH 6 (±0.5)

#	Name	Isotope labeling	Concentration
7	Fusion protein	[U-99% 13C; U-99% 15N]	500 uM
8	sodium phosphate	natural abundance	20 mM
9	sodium chloride	natural abundance	150 mM
10	DTT	natural abundance	1 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

7 3D 1H-15N NOESY

Instrument

Bruker DRX - 600 MHz TCI Cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 291 K, pH 6 (±0.5)

#	Name	Isotope labeling	Concentration
7	Fusion protein	[U-99% 13C; U-99% 15N]	500 uM
8	sodium phosphate	natural abundance	20 mM
9	sodium chloride	natural abundance	150 mM
10	DTT	natural abundance	1 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

8 3D HNHA

Instrument

Bruker DRX - 600 MHz TCI Cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 291 K, pH 6 (±0.5)

#	Name	Isotope labeling	Concentration
7	Fusion protein	[U-99% 13C; U-99% 15N]	500 uM
8	sodium phosphate	natural abundance	20 mM
9	sodium chloride	natural abundance	150 mM
10	DTT	natural abundance	1 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

9 3D C(CO)NH

Instrument

Bruker DRX - 600 MHz TCI Cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 291 K, pH 6 (±0.5)

#	Name	Isotope labeling	Concentration
7	Fusion protein	[U-99% 13C; U-99% 15N]	500 uM
8	sodium phosphate	natural abundance	20 mM
9	sodium chloride	natural abundance	150 mM
10	DTT	natural abundance	1 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

10 3D HCCH-TOCSY

Instrument

Bruker Avance - 800 MHz TCI Cryoprobe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 291 K, pH 6 (±0.5)

#	Name	Isotope labeling	Concentration
13	Fusion protein	[U-99% 13C; U-99% 15N]	300 uM
14	sodium phosphate	natural abundance	20 mM
15	sodium chloride	natural abundance	150 mM
16	DTT	natural abundance	1 mM
17	D2O	natural abundance	10 %

11 3D CCH-TOCSY

Instrument

Bruker Avance - 800 MHz TCI Cryoprobe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 291 K, pH 6 (±0.5)

#	Name	Isotope labeling	Concentration
13	Fusion protein	[U-99% 13C; U-99% 15N]	300 uM
14	sodium phosphate	natural abundance	20 mM
15	sodium chloride	natural abundance	150 mM
16	DTT	natural abundance	1 mM
17	D2O	natural abundance	10 %

12 3D 1H-13C NOESY

Instrument

Bruker DRX - 600 MHz TCI Cryoprobe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 291 K, pH 6 (±0.5)

#	Name	Isotope labeling	Concentration
13	Fusion protein	[U-99% 13C; U-99% 15N]	300 uM
14	sodium phosphate	natural abundance	20 mM
15	sodium chloride	natural abundance	150 mM
16	DTT	natural abundance	1 mM
17	D2O	natural abundance	10 %

13 2D 1H-1H NOESY

Instrument

Bruker DRX - 600 MHz TCI Cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 291 K, pH 6 (±0.5)

#	Name	Isotope labeling	Concentration
18	Fusion protein	natural abundance	500 uM
19	sodium phosphate	natural abundance	20 mM
20	sodium chloride	natural abundance	150 mM
21	DTT	natural abundance	1 mM
22	H2O	natural abundance	90 %
23	D2O	natural abundance	10 %

14 2D 1H-1H TOCSY

Instrument

Bruker DRX - 600 MHz TCI Cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 291 K, pH 6 (±0.5)

#	Name	Isotope labeling	Concentration
18	Fusion protein	natural abundance	500 uM
19	sodium phosphate	natural abundance	20 mM
20	sodium chloride	natural abundance	150 mM
21	DTT	natural abundance	1 mM
22	H2O	natural abundance	90 %
23	D2O	natural abundance	10 %

nullKeywords LIM domain, LIM-interaction domain, LIM-only protein, LMO4, protein-protein interaction, RBBP8/CtIP

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Found 1 Document (0.858 seconds)

BMRB: 17265

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Fusion protein, : 1 : 1 entities Detail

Experiments performed 14 experiments Detail

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