Solution structure of the C-terminal domain of SilB from Cupriavidus metallidurans
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 95.9 % (862 of 899) | 95.9 % (446 of 465) | 95.2 % (337 of 354) | 98.8 % (79 of 80) |
Backbone | 95.6 % (455 of 476) | 95.8 % (158 of 165) | 94.9 % (225 of 237) | 97.3 % (72 of 74) |
Sidechain | 94.6 % (469 of 496) | 94.3 % (283 of 300) | 94.7 % (180 of 190) | 100.0 % (6 of 6) |
Aromatic | 96.3 % (52 of 54) | 100.0 % (27 of 27) | 92.3 % (24 of 26) | 100.0 % (1 of 1) |
Methyl | 94.8 % (91 of 96) | 93.8 % (45 of 48) | 95.8 % (46 of 48) |
1. SilB(440-521)
GPEHRAVGRI QSIGERSLII AHEAIPSAQW GAMTMEFAAP PAGLPQGLKA GDRVAFSFRL DPHGMATLVT VAPQVQTAGA KPSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | SilB(440-521) | [U-100% 15N] | 1 mM | |
2 | MES | natural abundance | 50 mM | |
3 | H2O | natural abundance | 90 % | |
4 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | SilB(440-521) | [U-100% 13C; U-100% 15N] | 1 mM | |
6 | MES | natural abundance | 50 mM | |
7 | H2O | natural abundance | 90 % | |
8 | D2O | natural abundance | 10 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | SilB(440-521) | [U-100% 15N] | 1 mM | |
10 | MES | natural abundance | 50 mM | |
11 | H2O | natural abundance | 90 % | |
12 | D2O | natural abundance | 10 % |