BMRBj - BMREntryMaster

Found 1 Document (0.743 seconds)

BMRB: 17270

2L5E

Complex between BD1 of Brd3 and GATA-1 C-tail

Authors

Gamsjaeger, R., Webb, S.R., Lamonica, J.M., Blobel, G.A., Mackay, J.P.

Assembly

Brd3 in complex with GATA-1 C-tail

Entity

1. Brd3 (polymer, Thiol state: all free), 128 monomers, 15008.18 Da Detail

GPLGSEVSNP SKPGRKTNQL QYMQNVVVKT LWKHQFAWPF YQPVDAIKLN LPDYHKIIKN PMDMGTIKKR LENNYYWSAS ECMQDFNTMF TNCYIYNKPT DDIVLMAQAL EKIFLQKVAQ MPQEEVEL

2. GATA-1 C-tail (polymer, Thiol state: all free), 13 monomers, 1414.611 Da Detail

KASGXGKXLR GSN

Total weight

16422.791 Da

Max. entity weight

15008.18 Da

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.3 %, Completeness: 86.5 %, Completeness (bb): 92.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.5 % (1480 of 1711)	82.9 % (748 of 902)	90.5 % (593 of 655)	90.3 % (139 of 154)
Backbone	92.4 % (754 of 816)	93.5 % (258 of 276)	92.2 % (378 of 410)	90.8 % (118 of 130)
Sidechain	82.8 % (850 of 1027)	78.3 % (490 of 626)	89.9 % (339 of 377)	87.5 % (21 of 24)
Aromatic	78.0 % (117 of 150)	70.7 % (53 of 75)	84.7 % (61 of 72)	100.0 % (3 of 3)
Methyl	90.8 % (118 of 130)	89.2 % (58 of 65)	92.3 % (60 of 65)

1. Brd3

GPLGSEVSNP SKPGRKTNQL QYMQNVVVKT LWKHQFAWPF YQPVDAIKLN LPDYHKIIKN PMDMGTIKKR LENNYYWSAS ECMQDFNTMF TNCYIYNKPT DDIVLMAQAL EKIFLQKVAQ MPQEEVEL

2. GATA-1 C-tail

KASGXGKXLR GSN

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Brd3	[U-13C; U-15N]	0.5 ~ 1.5 mM
2	GATA-1_C-tail	natural abundance	0.5 ~ 1.5 mM
3	NaCl	natural abundance	100 mM
4	Tris	natural abundance	20 mM
5	DTT	natural abundance	1 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
8	Brd3	[U-13C; U-15N]	0.5 ~ 1.5 mM
9	GATA-1_C-tail	natural abundance	0.5 ~ 1.5 mM
10	NaCl	natural abundance	100 mM
11	Tris	natural abundance	20 mM
12	DTT	natural abundance	1 mM
13	D2O	natural abundance	100 %

Release date

2011-05-17

Citation

Structural basis and specificity of acetylated transcription factor GATA1 recognition by BET family bromodomain protein Brd3
Gamsjaeger, R., Webb, S.R., Lamonica, J.M., Billin, A., Blobel, G.A., Mackay, J.P.
Mol. Cell. Biol. (2011), 31, 2632-2640, PubMed 21555453 , DOI 10.1128/MCB.05413-11 , Abstract

Related entities 1. Brd3, : 3 : 80 entities Detail

Experiments performed 19 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Brd3	[U-13C; U-15N]	0.5 ~ 1.5 mM
2	GATA-1_C-tail	natural abundance	0.5 ~ 1.5 mM
3	NaCl	natural abundance	100 mM
4	Tris	natural abundance	20 mM
5	DTT	natural abundance	1 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Brd3	[U-13C; U-15N]	0.5 ~ 1.5 mM
2	GATA-1_C-tail	natural abundance	0.5 ~ 1.5 mM
3	NaCl	natural abundance	100 mM
4	Tris	natural abundance	20 mM
5	DTT	natural abundance	1 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

3 3D CBCA(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Brd3	[U-13C; U-15N]	0.5 ~ 1.5 mM
2	GATA-1_C-tail	natural abundance	0.5 ~ 1.5 mM
3	NaCl	natural abundance	100 mM
4	Tris	natural abundance	20 mM
5	DTT	natural abundance	1 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

4 3D HNCACB

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Brd3	[U-13C; U-15N]	0.5 ~ 1.5 mM
2	GATA-1_C-tail	natural abundance	0.5 ~ 1.5 mM
3	NaCl	natural abundance	100 mM
4	Tris	natural abundance	20 mM
5	DTT	natural abundance	1 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

5 3D HNCO

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Brd3	[U-13C; U-15N]	0.5 ~ 1.5 mM
2	GATA-1_C-tail	natural abundance	0.5 ~ 1.5 mM
3	NaCl	natural abundance	100 mM
4	Tris	natural abundance	20 mM
5	DTT	natural abundance	1 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

6 3D HN(CO)CA

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Brd3	[U-13C; U-15N]	0.5 ~ 1.5 mM
2	GATA-1_C-tail	natural abundance	0.5 ~ 1.5 mM
3	NaCl	natural abundance	100 mM
4	Tris	natural abundance	20 mM
5	DTT	natural abundance	1 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

7 3D HBHA(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Brd3	[U-13C; U-15N]	0.5 ~ 1.5 mM
2	GATA-1_C-tail	natural abundance	0.5 ~ 1.5 mM
3	NaCl	natural abundance	100 mM
4	Tris	natural abundance	20 mM
5	DTT	natural abundance	1 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

8 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Brd3	[U-13C; U-15N]	0.5 ~ 1.5 mM
2	GATA-1_C-tail	natural abundance	0.5 ~ 1.5 mM
3	NaCl	natural abundance	100 mM
4	Tris	natural abundance	20 mM
5	DTT	natural abundance	1 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

9 3D HCCH-TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
8	Brd3	[U-13C; U-15N]	0.5 ~ 1.5 mM
9	GATA-1_C-tail	natural abundance	0.5 ~ 1.5 mM
10	NaCl	natural abundance	100 mM
11	Tris	natural abundance	20 mM
12	DTT	natural abundance	1 mM
13	D2O	natural abundance	100 %

10 3D (H)CCH-TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
8	Brd3	[U-13C; U-15N]	0.5 ~ 1.5 mM
9	GATA-1_C-tail	natural abundance	0.5 ~ 1.5 mM
10	NaCl	natural abundance	100 mM
11	Tris	natural abundance	20 mM
12	DTT	natural abundance	1 mM
13	D2O	natural abundance	100 %

11 3D HNHA

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Brd3	[U-13C; U-15N]	0.5 ~ 1.5 mM
2	GATA-1_C-tail	natural abundance	0.5 ~ 1.5 mM
3	NaCl	natural abundance	100 mM
4	Tris	natural abundance	20 mM
5	DTT	natural abundance	1 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

12 3D HCC(CO)NH TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Brd3	[U-13C; U-15N]	0.5 ~ 1.5 mM
2	GATA-1_C-tail	natural abundance	0.5 ~ 1.5 mM
3	NaCl	natural abundance	100 mM
4	Tris	natural abundance	20 mM
5	DTT	natural abundance	1 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

13 3D CC(CO)NH TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Brd3	[U-13C; U-15N]	0.5 ~ 1.5 mM
2	GATA-1_C-tail	natural abundance	0.5 ~ 1.5 mM
3	NaCl	natural abundance	100 mM
4	Tris	natural abundance	20 mM
5	DTT	natural abundance	1 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

14 3D 13C sep (F2) 15N13C filtered (F1) NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Brd3	[U-13C; U-15N]	0.5 ~ 1.5 mM
2	GATA-1_C-tail	natural abundance	0.5 ~ 1.5 mM
3	NaCl	natural abundance	100 mM
4	Tris	natural abundance	20 mM
5	DTT	natural abundance	1 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

15 3D 13C sep (F1) 15N13C filtered (F3) NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Brd3	[U-13C; U-15N]	0.5 ~ 1.5 mM
2	GATA-1_C-tail	natural abundance	0.5 ~ 1.5 mM
3	NaCl	natural abundance	100 mM
4	Tris	natural abundance	20 mM
5	DTT	natural abundance	1 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

16 2D 15N13C filtered NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Brd3	[U-13C; U-15N]	0.5 ~ 1.5 mM
2	GATA-1_C-tail	natural abundance	0.5 ~ 1.5 mM
3	NaCl	natural abundance	100 mM
4	Tris	natural abundance	20 mM
5	DTT	natural abundance	1 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

17 2D 15N13C filtered TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Brd3	[U-13C; U-15N]	0.5 ~ 1.5 mM
2	GATA-1_C-tail	natural abundance	0.5 ~ 1.5 mM
3	NaCl	natural abundance	100 mM
4	Tris	natural abundance	20 mM
5	DTT	natural abundance	1 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

18 3D 13C NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
8	Brd3	[U-13C; U-15N]	0.5 ~ 1.5 mM
9	GATA-1_C-tail	natural abundance	0.5 ~ 1.5 mM
10	NaCl	natural abundance	100 mM
11	Tris	natural abundance	20 mM
12	DTT	natural abundance	1 mM
13	D2O	natural abundance	100 %

19 3D 15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Brd3	[U-13C; U-15N]	0.5 ~ 1.5 mM
2	GATA-1_C-tail	natural abundance	0.5 ~ 1.5 mM
3	NaCl	natural abundance	100 mM
4	Tris	natural abundance	20 mM
5	DTT	natural abundance	1 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

nullKeywords Acetylation, Brd3, GATA-1, histone code, NMR

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Found 1 Document (0.743 seconds)

BMRB: 17270

Sample #1

Sample #2

Related entities 1. Brd3, : 3 : 80 entities Detail

Experiments performed 19 experiments Detail

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