BMRB: 17271

Co-ordinates and 1H, 13C and 15N chemical shift assignments for the complex of GPS2 53-90 and SMRT 167-207

Authors

Yang, J., Oberoi, J., Neuhaus, D., Schwabe, J.W.R.

Assembly

complex of GPS2 53-90 and SMRT 167-207

Entity

1. GPS2 53-90 (polymer, Thiol state: not present), 38 monomers, 4684.424 Da Detail

KKEMEERMSL EETKEQILKL EEKLLALQEE KHQLFLQL

2. SMRT 167-207 (polymer, Thiol state: not present), 42 monomers, 4940.602 Da Detail

GLSKEELIQN MDRVDREITM VEQQISKLKK KQQQLEEEAA KP

Total weight

9625.025 Da

Max. entity weight

4940.602 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 80.7 %, Completeness (bb): 83.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	80.7 % (828 of 1026)	89.5 % (496 of 554)	63.9 % (244 of 382)	97.8 % (88 of 90)
Backbone	83.9 % (401 of 478)	98.8 % (158 of 160)	69.5 % (166 of 239)	97.5 % (77 of 79)
Sidechain	80.5 % (505 of 627)	85.8 % (338 of 394)	70.3 % (156 of 222)	100.0 % (11 of 11)
Aromatic	100.0 % (14 of 14)	100.0 % (7 of 7)	100.0 % (7 of 7)
Methyl	100.0 % (86 of 86)	100.0 % (43 of 43)	100.0 % (43 of 43)

1. GPS2 53-90

KKEMEERMSL EETKEQILKL EEKLLALQEE KHQLFLQL

2. SMRT 167-207

GLSKEELIQN MDRVDREITM VEQQISKLKK KQQQLEEEAA KP

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 285 K, pH 6.5, Details 20 mM deuterated Tris pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	GPS2_53-90	[U-98% 13C; U-98% 15N]		0.4 mM
2	SMRT_167-207	[U-98% 13C; U-98% 15N]		0.4 mM
3	Tris	natural abundance		20 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 285 K, pH 6.5, Details 20 mM deuterated Tris pH 6.5

#	Name	Isotope labeling	Type	Concentration
6	SMRT_167-207	[U-98% 13C; U-98% 15N]		0.4 mM
7	GPS2_53-90	natural abundance		0.4 mM
8	Tris	natural abundance		20 mM
9	H2O	natural abundance		90 %
10	D2O	natural abundance		10 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 285 K, pH 6.5, Details 20 mM deuterated Tris pH 6.5

#	Name	Isotope labeling	Type	Concentration
11	GPS2_53-90	[U-98% 13C; U-98% 15N]		0.4 mM
12	SMRT_167-207	natural abundance		0.4 mM
13	Tris	natural abundance		20 mM
14	H2O	natural abundance		90 %
15	D2O	natural abundance		10 %

Hide sample codition

Protein Blocks Logo

Calculated from 35 models in PDB: 2L5G, Strand ID: A, B Detail

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Release date

2011-01-17

Citation

Structural basis for the assembly of the SMRT/NCoR core transcriptional repression machinery
Oberoi, J., Fairall, L., Watson, P.J., Yang, J., Czimmerer, Z., Kampmann, T., Goult, B.T., Greenwood, J.A., Gooch, J.T., Kallenberger, B.C., Nagy, L., Neuhaus, D., Schwabe, J.W.R.
Nat. Struct. Mol. Biol. (2011), 18, 177-184, PubMed 21240272 , DOI 10.1038/nsmb.1983 , Abstract

Related entities 1. GPS2 53-90, : 1 : 2 : 10 entities Detail

Related entities 2. SMRT 167-207, : 1 : 5 : 13 entities Detail

Interaction partners 1. GPS2 53-90, : 56 : 1 interactors Detail

Interaction partners 2. SMRT 167-207, : 40 : 1 interactors Detail

Experiments performed 25 experiments Detail