BMRB: 17272

Solution structures of human PIWI-like 1 PAZ domain

Authors

Zeng, L., Zhang, Q., Yan, K., Zhou, M.

Assembly

Human PIWI-like 1 PAZ domain

Entity

1. Human PIWI-like 1 PAZ domain (polymer, Thiol state: not available), 134 monomers, 15651.57 Da Detail

CTDVSHKVLR SETVLDFMFN FYHQTEEHKF QEQVSKELIG LVVLTKYNNK TYRVDDIDWD QNPKSTFKKA DGSEVSFLEY YRKQYNQEIT DLKQPVLVSQ PKRRRGPGGT LPGPAMLIPE LCYLTGLTDK MRND

Formula weight

15651.57 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 83.8 %, Completeness (bb): 84.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	83.8 % (1367 of 1631)	87.6 % (751 of 857)	75.8 % (479 of 632)	96.5 % (137 of 142)
Backbone	84.9 % (671 of 790)	99.3 % (266 of 268)	70.9 % (280 of 395)	98.4 % (125 of 127)
Sidechain	84.9 % (822 of 968)	82.3 % (485 of 589)	89.3 % (325 of 364)	80.0 % (12 of 15)
Aromatic	62.1 % (87 of 140)	64.3 % (45 of 70)	59.4 % (41 of 69)	100.0 % (1 of 1)
Methyl	100.0 % (132 of 132)	100.0 % (66 of 66)	100.0 % (66 of 66)

1. Human PIWI-like 1 PAZ domain

CTDVSHKVLR SETVLDFMFN FYHQTEEHKF QEQVSKELIG LVVLTKYNNK TYRVDDIDWD QNPKSTFKKA DGSEVSFLEY YRKQYNQEIT DLKQPVLVSQ PKRRRGPGGT LPGPAMLIPE LCYLTGLTDK MRND

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	sodium phosphate	natural abundance		100 mM
2	sodium chloride	natural abundance		150 mM
3	DTT	[U-100% 2H]		5 mM
4	D2O	natural abundance		100 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
5	sodium phosphate	natural abundance		100 mM
6	sodium chloride	natural abundance		150 mM
7	DTT	[U-100% 2H]		5 mM
8	D2O	natural abundance		10 %
9	H2O	natural abundance		90 %

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LACS Plot; CA

Referencing offset: -0.02 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	water	protons	4.85 ppm	internal	direct	1.0
15N	na	na	0.0 ppm	null	null	0.0

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LACS Plot; CB

Referencing offset: -0.02 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	water	protons	4.85 ppm	internal	direct	1.0
15N	na	na	0.0 ppm	null	null	0.0

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LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	water	protons	4.85 ppm	internal	direct	1.0
15N	na	na	0.0 ppm	null	null	0.0

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Protein Blocks Logo

Calculated from 20 models in PDB: 2L5C, Strand ID: A Detail

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Release date

2011-04-07

Citation

Structural insights into piRNA recognition by the human PIWI-like 1 PAZ domain
Zeng, L., Zhang, Q., Yan, K., Zhou, M.
Proteins (2011), 79, 2004-2009, PubMed 21465557 , DOI 10.1002/prot.23003 , Abstract

Related entities 1. Human PIWI-like 1 PAZ domain, : 1 : 3 : 3 : 44 entities Detail

Interaction partners 1. Human PIWI-like 1 PAZ domain, : 12 interactors Detail

Experiments performed 5 experiments Detail