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BMRB: 17278

2MOT

NMR assignment of actin depolymerizing and dynamics regulatory protein from toxoplasma gondii

Authors

Shukla, V., Pathak, P., Yadav, R., Srivastava, S., Jain, A., Pulavarti, S.

Assembly

TgADF Monomer

Entity

1. TgADF Monomer (polymer, Thiol state: all free), 118 monomers, 12947.86 Da Detail

MASGMGVDEN CVARFNELKI RKTVKWIVFK IENTKIVVEK DGKGNADEFR GALPANDCRF GVYDCGNKIQ FVLWCPDNAP VKPRMTYASS KDALLKKLDG ATAVALEAHE MGDLAPLA

Formula weight

12947.86 Da

Source organism

Toxoplasma gondii

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.3 %, Completeness: 88.6 %, Completeness (bb): 95.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.6 % (1200 of 1355)	86.2 % (607 of 704)	91.7 % (484 of 528)	88.6 % (109 of 123)
Backbone	95.3 % (665 of 698)	91.3 % (219 of 240)	98.3 % (339 of 345)	94.7 % (107 of 113)
Sidechain	83.9 % (643 of 766)	83.8 % (389 of 464)	86.3 % (252 of 292)	20.0 % (2 of 10)
Aromatic	59.6 % (56 of 94)	68.1 % (32 of 47)	53.3 % (24 of 45)	0.0 % (0 of 2)
Methyl	93.9 % (124 of 132)	95.5 % (63 of 66)	92.4 % (61 of 66)

1. TgADF

MASGMGVDEN CVARFNELKI RKTVKWIVFK IENTKIVVEK DGKGNADEFR GALPANDCRF GVYDCGNKIQ FVLWCPDNAP VKPRMTYASS KDALLKKLDG ATAVALEAHE MGDLAPLA

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	potassium phosphate	natural abundance	20 mM
2	sodium chloride	natural abundance	50 mM
3	sodium azide	natural abundance	0.1 %
4	DTT	natural abundance	1 mM
5	AEBSF protease inhibitor	natural abundance	1 mM
6	H2O	natural abundance	95 %
7	D2O	natural abundance	5 %
8	TgADF	[U-100% 13C; U-100% 15N]	0.8 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.55 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MOT, Strand ID: A Detail

Release date

2010-11-14

Citation

NMR assignment of actin depolymerizing and dynamics regulatory protein from Toxoplasma gondii
Shukla, V., Pathak, P., Yadav, R., Srivastava, S., Jain, A., Pulavarti, S., Arora, A.
Biomol. NMR Assignments

Related entities 1. TgADF Monomer, : 1 : 1 : 193 entities Detail

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC