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BMRB: 17279

2L5P

Solution NMR structure of protein lipocalin 12 from rat epididymis

Authors

Peng, Y., Lin, D.

Assembly

rat epididymal lipocalin 12

Entity

1. rat epididymal lipocalin 12 (polymer, Thiol state: all disulfide bound), 184 monomers, 21294.90 Da Detail

MGQSPTMPQG FSQMTSFQSN KFQGEWFVLG LADNTYKREH RPLLHSFITL FKLRDNSEFQ VTNSMTRGKH CSTWSYTLIP TNKPGQFTRD NRGSGPGADK ENIQVIETDY VKFALVLSLR QASNQNITRV SLLGRDWKIT HKTIDRFIAL TKTQNLTKNN LLFPDLTDWL LDPKVCLEHH HHHH

Formula weight

21294.9 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS71:SG	1:CYS176:SG

Source organism

Rattus norvegicus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.7 %, Completeness: 84.1 %, Completeness (bb): 90.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	84.1 % (1872 of 2226)	81.3 % (944 of 1161)	86.0 % (741 of 862)	92.1 % (187 of 203)
Backbone	90.6 % (986 of 1088)	91.1 % (337 of 370)	89.9 % (487 of 542)	92.0 % (162 of 176)
Sidechain	79.3 % (1040 of 1312)	76.4 % (604 of 791)	83.4 % (412 of 494)	88.9 % (24 of 27)
Aromatic	55.9 % (124 of 222)	56.8 % (63 of 111)	53.3 % (57 of 107)	100.0 % (4 of 4)
Methyl	88.7 % (165 of 186)	88.2 % (82 of 93)	89.2 % (83 of 93)

1. rat epididymal lipocalin 12

Show sample condition

Sample

Solvent system 90% H2O, 10% D2O, Pressure 1 Pa, Temperature 310 K, pH 4.5, Details 20MM NaAC;0.5MM EDTA; 90% H2O, 10% D2O

#	Name	Isotope labeling	Concentration
1	rLcn12	[U-100% 13C; U-100% 15N]	0.5 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	NaAC	natural abundance	20 mM
5	EDTA	natural abundance	0.5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.18 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.49 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L5P, Strand ID: A Detail

Release date

2011-05-04

Citation

Solution structure of the protein lipocalin 12 from rat epididymis
Peng, Y., Zhang, X., Liu, J., Liu, Q., Guo, C., Zhang, Y., Lin, D.
Proteins (2011), 79, 2316-2320, PubMed 21538546 , DOI 10.1002/prot.23031 , Abstract

Entries sharing articles Swiss-Prot: 2 entries Detail

  Swiss-Prot: B3EY83 released on 2012-03-21
    Title LCN12_RAT Entity Epididymal-specific lipocalin-12
  Swiss-Prot: P30152 released on 1993-04-01
    Title NGAL_RAT Entity Neutrophil gelatinase-associated lipocalin

Related entities 1. rat epididymal lipocalin 12, : 1 : 2 : 167 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC