BMRB: 17284

Solution NMR structure of E2 lipoyl domain from Thermoplasma acidophilum

Authors

Bagby, S., Posner, M., Upadhyay, A., Danson, M.

Assembly

E2 lipoyl domain

Entity

1. E2 lipoyl domain (polymer, Thiol state: not present), 77 monomers, 8660.932 Da Detail

MYEFKLPDIG EGVTEGEIVR WDVKEGDMVE KDQDLVEVMT DKVTVKIPSP VRGKIVKILY REGQVVPVGS TLLQIDT

Formula weight

8660.932 Da

Source organism

Thermoplasma acidophilum

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 91.6 %, Completeness (bb): 96.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.6 % (831 of 907)	92.2 % (435 of 472)	90.8 % (325 of 358)	92.2 % (71 of 77)
Backbone	96.3 % (437 of 454)	94.9 % (149 of 157)	97.3 % (218 of 224)	95.9 % (70 of 73)
Sidechain	86.8 % (454 of 523)	88.3 % (278 of 315)	85.8 % (175 of 204)	25.0 % (1 of 4)
Aromatic	36.8 % (14 of 38)	57.9 % (11 of 19)	11.1 % (2 of 18)	100.0 % (1 of 1)
Methyl	92.5 % (98 of 106)	94.3 % (50 of 53)	90.6 % (48 of 53)

1. E2 lipoyl domain

MYEFKLPDIG EGVTEGEIVR WDVKEGDMVE KDQDLVEVMT DKVTVKIPSP VRGKIVKILY REGQVVPVGS TLLQIDT

Show sample condition

Sample

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 310 K, pH 7.5, Details pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	sodium chloride	natural abundance		50 mM
2	HEPES	natural abundance		50 mM
3	H2O	natural abundance		93 %
4	D2O	natural abundance		7 %
5	E2_lipoyl_domain	[U-13C; U-15N]		1 mM

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LACS Plot; CA

Referencing offset: -0.74 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.74 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.06 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 1.63 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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Protein Blocks Logo

Calculated from 33 models in PDB: 2L5T, Strand ID: A Detail

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Release date

2012-07-24

Citation

A unique lipoylation system in the Archaea. Lipoylation in Thermoplasma acidophilum requires two proteins
Posner, M., Upadhyay, A., Danson, M., Bagby, S.
FEBS J. (2009), 276, 4012-4022, PubMed 19594830 , DOI 10.1111/j.1742-4658.2009.07110.x , Abstract

Entries sharing articles Swiss-Prot: 2 entries Detail

Related entities 1. E2 lipoyl domain, : 1 : 231 entities Detail

Experiments performed 10 experiments Detail