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BMRB: 17286

2L5V

Solution structure of the C-terminal domain of hRpn13

Authors

Zhou, Z., Hu, H.

Assembly

hRpn13

Entity

1. hRpn13 (polymer, Thiol state: all free), 150 monomers, 15796.53 Da Detail

GSQPIQLSDL QSILATMNVP AGPAGGQQVD LASVLTPEIM APILANADVQ ERLLPYLPSG ESLPQTADEI QNTLTSPQFQ QALGMFSAAL ASGQLGPLMC QFGLPAEAVE AANKGDVEAF AKAMQNNAKP EQKEGDTKDK KDEEEDMSLD

Formula weight

15796.53 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.3 %, Completeness: 81.8 %, Completeness (bb): 96.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	81.8 % (1353 of 1654)	77.0 % (664 of 862)	87.7 % (555 of 633)	84.3 % (134 of 159)
Backbone	96.0 % (841 of 876)	95.7 % (286 of 299)	96.1 % (422 of 439)	96.4 % (133 of 138)
Sidechain	70.7 % (648 of 917)	67.3 % (379 of 563)	80.5 % (268 of 333)	4.8 % (1 of 21)
Aromatic	72.9 % (35 of 48)	79.2 % (19 of 24)	66.7 % (16 of 24)
Methyl	84.8 % (139 of 164)	89.0 % (73 of 82)	80.5 % (66 of 82)

1. entity

GSQPIQLSDL QSILATMNVP AGPAGGQQVD LASVLTPEIM APILANADVQ ERLLPYLPSG ESLPQTADEI QNTLTSPQFQ QALGMFSAAL ASGQLGPLMC QFGLPAEAVE AANKGDVEAF AKAMQNNAKP EQKEGDTKDK KDEEEDMSLD

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	hRpn13	natural abundance	20 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	DTT	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.18 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.18 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L5V, Strand ID: A Detail

Release date

2012-07-24

Citation

Solution structure of the C-terminal domain of hRpn13
Zhou, Z., Hu, H.
Not known

Related entities 1. hRpn13, : 1 : 2 : 25 entities Detail

Interaction partners 1. hRpn13, : 47 interactors Detail

More details for 47 interactors identified by 20 citations

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC