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BMRB: 17287

2LLA

NMR solution structure ensemble of domain 11 of the echidna M6P/IGF2R receptor

Authors

Strickland, M., Crump, M.P., Williams, C., Rezgui, D., Ellis, R.Z., Hoppe, H., Frago, S., Prince, S.N., Zaccheo, O.J., Forbes, B.E., Jones, E., Hassan, A.Z., Wattana-Amorn, P.

Assembly

M6P/IGF2 receptor

Entity

1. M6P/IGF2 receptor (polymer, Thiol state: all disulfide bound), 140 monomers, 15283.18 Da Detail

MVQDNCQVTN PATGYVFDLN SLKRESGYTI SDIRKGSIRL GVCGEVKDCG PGIGACFEGT GIKAGKWNQK LSYVDQVLQL VYEDGDPCPA NLHLKYKSVI SFVCKSDAGP TSQPLLLSVD EHTCTLFFSW HTSLACEQEV

Formula weight

15283.18 Da

Entity Connection

disulfide 4 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS6:SG	1:CYS43:SG
2	disulfide	sing	1:CYS124:SG	1:CYS88:SG
3	disulfide	sing	1:CYS49:SG	1:CYS56:SG
4	disulfide	sing	1:CYS136:SG	1:CYS104:SG

Source organism

Tachyglossus aculeatus

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.3 %, Completeness (bb): 98.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	95.3 % (1507 of 1581)	95.8 % (781 of 815)	93.7 % (579 of 618)	99.3 % (147 of 148)
Backbone	98.7 % (817 of 828)	98.6 % (283 of 287)	98.3 % (400 of 407)	100.0 % (134 of 134)
Sidechain	92.8 % (817 of 880)	94.3 % (498 of 528)	90.5 % (306 of 338)	92.9 % (13 of 14)
Aromatic	69.0 % (87 of 126)	76.2 % (48 of 63)	62.3 % (38 of 61)	50.0 % (1 of 2)
Methyl	97.4 % (148 of 152)	98.7 % (75 of 76)	96.1 % (73 of 76)

1. entity

MVQDNCQVTN PATGYVFDLN SLKRESGYTI SDIRKGSIRL GVCGEVKDCG PGIGACFEGT GIKAGKWNQK LSYVDQVLQL VYEDGDPCPA NLHLKYKSVI SFVCKSDAGP TSQPLLLSVD EHTCTLFFSW HTSLACEQEV

Show sample condition

Sample

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298.15 K, pH 5.50

#	Name	Isotope labeling	Concentration
1	sodium azide	natural abundance	100 uM
2	sodium phosphate	natural abundance	10 mM
3	EDTA	natural abundance	1 mM
4	D2O	natural abundance	7 %
5	H2O	natural abundance	93 %
6	M6P/IGF2_receptor	[U-95% 13C; U-95% 15N]	0.5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.78 ppm	internal	indirect	0.2514495
¹H	water	protons	4.78 ppm	internal	direct	1.0
¹⁵N	water	protons	4.78 ppm	internal	indirect	0.1013291

Referencing offset: 0.71 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.78 ppm	internal	indirect	0.2514495
¹H	water	protons	4.78 ppm	internal	direct	1.0
¹⁵N	water	protons	4.78 ppm	internal	indirect	0.1013291

Referencing offset: 0.71 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.78 ppm	internal	indirect	0.2514495
¹H	water	protons	4.78 ppm	internal	direct	1.0
¹⁵N	water	protons	4.78 ppm	internal	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.78 ppm	internal	indirect	0.2514495
¹H	water	protons	4.78 ppm	internal	direct	1.0
¹⁵N	water	protons	4.78 ppm	internal	indirect	0.1013291

Referencing offset: 0.44 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LLA, Strand ID: A Detail

Release date

2012-11-04

Citation

An exon splice enhancer primes IGF2:IGF2R binding site structure and function evolution
Williams, C., Hoppe, H., Rezgui, D., Strickland, M., Frago, S., Ellis, R.Z., Wattana-Amorn, P., Prince, S.N., Zaccheo, O.J., Forbes, B.E., Jones, E.YVONNE., Crump, M.P., Hassan, A.BASSIM., Grutzner, F., Nolan, C.M., Mungall, A.J.
Science (2012), 338, 1209-1213, PubMed 23197533 , DOI 10.1126/science.1228633 , Abstract

Entries sharing articles BMRB: 4 entries Detail

  BMRB: 17128 released on 2014-03-03
    Title unbound Cation-independent mannose-6-phosphate receptor domain 11 E4 mutant
  BMRB: 17134 released on 2014-03-03
    Title Solution structure of Opossum Domain 11
  BMRB: 17110 released on 2012-08-29
    Title chicken IGF2R domain 11
  BMRB: 17127 released on 2012-08-29
    Title COMPLEX STRUCTURE OF E4 mutant HUMAN IGF2R DOMAIN 11 BOUND TO IGF-II

Related entities 1. M6P/IGF2 receptor, : 1 : 97 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC