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BMRB: 17288

2MF2

1H, 13C, and 15N backbone and side-chain chemical shift assignment of the staphylococcal toxin MazF

Authors

Zorzini, V., Cheung, A., Loris, R., van Nuland, N.A.J.

Assembly

SaMazF

Entity

1. SaMazF (polymer, Thiol state: not present), 132 monomers, 14791.77 Da Detail

GSSHHHHHHS QDPIRRGDVY LADLSPVQGS EQGGVRPVVI IQNDTGNKYS PTVIVAAITG RINKAKIPTH VEIEKKKYKL DKDSVILLEQ IRTLDKKRLK EKLTYLSDDK MKEVDNALMI SLGLNAVAHQ KN

Formula weight

14791.77 Da

Source organism

Staphylococcus aureus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.7 %, Completeness: 88.3 %, Completeness (bb): 90.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.3 % (1371 of 1553)	88.3 % (719 of 814)	88.2 % (529 of 600)	88.5 % (123 of 139)
Backbone	90.0 % (704 of 782)	89.5 % (239 of 267)	91.0 % (353 of 388)	88.2 % (112 of 127)
Sidechain	87.3 % (781 of 895)	87.8 % (480 of 547)	86.3 % (290 of 336)	91.7 % (11 of 12)
Aromatic	59.4 % (38 of 64)	62.5 % (20 of 32)	56.3 % (18 of 32)
Methyl	97.5 % (158 of 162)	100.0 % (81 of 81)	95.1 % (77 of 81)

1. SaMazF

GSSHHHHHHS QDPIRRGDVY LADLSPVQGS EQGGVRPVVI IQNDTGNKYS PTVIVAAITG RINKAKIPTH VEIEKKKYKL DKDSVILLEQ IRTLDKKRLK EKLTYLSDDK MKEVDNALMI SLGLNAVAHQ KN

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.6

#	Name	Isotope labeling	Concentration
1	SaMazF	[U-99% 13C; U-99% 15N]	1 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.32 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.32 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.26 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MF2, Strand ID: A, B Detail

Release date

2011-01-09

Citation

1H, 13C, and 15N backbone and side-chain chemical shift assignment of the staphylococcal MazF mRNA interferase
Zorzini, V., Haesaerts, S., Cheung, A., Loris, R., van Nuland, N.A.J.
Biomol. NMR Assign. (2011), 5, 157-160, PubMed 21213075 , DOI 10.1007/s12104-010-9290-1 , Abstract