BMRB: 17291

A novel design concept: New Y-receptor agonists with increased membrane recruitment, Y2 affinity and selectivity

Authors

Pedersen, S.L., Jurt, S., Zerbe, O., Jensen, K.J.

Assembly

PYY

Entity

1. PYY (polymer, Thiol state: not present), 40 monomers, 5112.843 Da Detail

YLERELKKLE RELKKLSPEE LNRYYASLRH YLNLVTRQRY

Formula weight

5112.843 Da

Exptl. method

solution NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.6 %, Completeness (bb): 98.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	97.6 % (279 of 286)	97.6 % (279 of 286)
Backbone	98.7 % (78 of 79)	98.7 % (78 of 79)
Sidechain	97.1 % (201 of 207)	97.1 % (201 of 207)
Aromatic	100.0 % (22 of 22)	100.0 % (22 of 22)
Methyl	100.0 % (22 of 22)	100.0 % (22 of 22)

1. entity 1

YLERELKKLE RELKKLSPEE LNRYYASLRH YLNLVTRQRY

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	PYY	natural abundance		1 mM
2	DPC	natural abundance		300 mM
3	MES	natural abundance		20 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2L60, Strand ID: A Detail

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Release date

2014-03-03

Citation

Improving membrane binding as a design strategy for amphipathic peptide hormones: 2-helix variants of PYY3-36
Pedersen, S., Bhatia, V., Jurt, S., Pedersen, M., Stamou, D., Zerbe, O., Holst, B., Vrang, N., Jensen, K.
J. Pept. Sci. (2012), 18, 579-587, PubMed 22865741 , DOI 10.1002/psc.2436 , Abstract

Related entities 1. PYY, : 1 : 73 entities Detail

Experiments performed 2 experiments Detail