BMRB: 17294

Protein and metal cluster structure of the wheat metallothionein domain g-Ec-1. The second part of the puzzle.

Authors

Loebus, J., Peroza, E.A., Bluethgen, N., Fox, T., Meyer-Klaucke, W., Zerbe, O., FREISINGER, E.

Assembly

EC PROTEIN I/II

Entity

1. EC PROTEIN I/II (polymer, Thiol state: all other bound), 26 monomers, 2461.779 Da Detail

GSGCDDKCGC AVPCPGGTGC RCTSAR

2. CD (non-polymer), 112.411 × 2 Da

Total weight

2686.601 Da

Max. entity weight

2461.779 Da

Entity Connection

na 6 Detail

---

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS4:SG	2:CD1:CD
2	na	sing	1:CYS8:SG	2:CD1:CD
3	na	sing	1:CYS10:SG	2:CD1:CD
4	na	sing	1:CYS14:SG	2:CD1:CD
5	na	sing	1:CYS20:SG	2:CD1:CD
6	na	sing	1:CYS22:SG	2:CD1:CD

Source organism

Triticum aestivum

Exptl. method

solution NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.3 %, Completeness: 86.0 %, Completeness (bb): 80.0 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	15N
All	86.0 % (123 of 143)	86.6 % (103 of 119)	83.3 % (20 of 24)
Backbone	80.0 % (64 of 80)	78.6 % (44 of 56)	83.3 % (20 of 24)
Sidechain	93.7 % (59 of 63)	93.7 % (59 of 63)
Methyl	100.0 % (6 of 6)	100.0 % (6 of 6)

1. EC PROTEIN I/II

GSGCDDKCGC AVPCPGGTGC RCTSAR

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.9, Details 1.0 MM [U-99% 15N] EC PROTEIN, 15.0 MM TRIS, 50.0 MM NACL, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	EC PROTEIN I/II	[U-99% 15N]		1.0 mM
2	TRIS	[U-98% 2H]		15 mM
3	D2O	[U-99% 2H]		10 %
4	NaCl	natural abundance		50 mM
5	H2O	natural abundance		90 %

Hide sample codition

Protein Blocks Logo

Calculated from 40 models in PDB: 2L61, Strand ID: A Detail

---

Release date

2011-03-27

Citation

Protein and metal cluster structure of the wheat metallothionein domain γ-E(c)-1: the second part of the puzzle
Loebus, J., Peroza, E.A., Bluthgen, N., Fox, T., Meyer-Klaucke, W., Zerbe, O., Freisinger, E.
J. Biol. Inorg. Chem. (2011), 16, 683-694, PubMed 21437709 , DOI 10.1007/s00775-011-0770-2 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. EC PROTEIN I/II, : 1 : 1 : 1 : 15 entities Detail

Experiments performed 5 experiments Detail