BMRB: 17295

Protein and metal cluster structure of the wheat metallothionein domain g-Ec-1. The second part of the puzzle.

Authors

Loebus, J., Peroza, E.A., Bluethgen, N., Fox, T., Meyer-Klaucke, W., Zerbe, O., FREISINGER, E.

Assembly

EC PROTEIN I/II

Entity

1. EC PROTEIN I/II (polymer, Thiol state: not present), 26 monomers, 2461.779 Da Detail

GSGCDDKCGC AVPCPGGTGC RCTSAR

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

2592.5972 Da

Max. entity weight

2461.779 Da

Entity Connection

na 6 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS4:SG	2:ZN1:ZN
2	na	sing	1:CYS8:SG	2:ZN1:ZN
3	na	sing	1:CYS10:SG	2:ZN1:ZN
4	na	sing	1:CYS14:SG	2:ZN1:ZN
5	na	sing	1:CYS20:SG	2:ZN1:ZN
6	na	sing	1:CYS22:SG	2:ZN1:ZN

Source organism

Triticum aestivum

Exptl. method

solution NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.2 %, Completeness: 91.6 %, Completeness (bb): 90.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	91.6 % (131 of 143)	91.6 % (109 of 119)	91.7 % (22 of 24)
Backbone	90.0 % (72 of 80)	89.3 % (50 of 56)	91.7 % (22 of 24)
Sidechain	93.7 % (59 of 63)	93.7 % (59 of 63)
Methyl	100.0 % (6 of 6)	100.0 % (6 of 6)

1. EC PROTEIN I/II

GSGCDDKCGC AVPCPGGTGC RCTSAR

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.9, Details 1.0 MM [U-99% 15N] EC PROTEIN, 15.0 MM TRIS, 50.0 MM NACL, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	EC PROTEIN I/II	[U-99% 15N]		1.0 mM
2	TRIS	[U-98% 2H]		15 mM
3	D2O	[U-99% 2H]		10 %
4	NaCl	natural abundance		50 mM
5	H2O	natural abundance		90 %

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Protein Blocks Logo

Calculated from 40 models in PDB: 2L62, Strand ID: A Detail

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Release date

2011-03-27

Citation

Protein and metal cluster structure of the wheat metallothionein domain γ-E(c)-1: the second part of the puzzle
Loebus, J., Peroza, E.A., Bluthgen, N., Fox, T., Meyer-Klaucke, W., Zerbe, O., Freisinger, E.
J. Biol. Inorg. Chem. (2011), 16, 683-694, PubMed 21437709 , DOI 10.1007/s00775-011-0770-2 , Abstract

Related entities 1. EC PROTEIN I/II, : 1 : 1 : 1 : 15 entities Detail

Experiments performed 4 experiments Detail