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BMRB: 17298

2MDT

Resonance assignments of protein SSO1118 from hyperthermophilic archaeon Sulfolobus solfataricus P2

Authors

Feng, Y.

Assembly

sso1118

Entity

1. sso1118 (polymer, Thiol state: not present), 119 monomers, 13012.88 Da Detail

MFAVISPSAF GKLKEILGSN KNYKFVITTL GVSFAIKSGI DIDSALDRGV IVRAFSHKPP KVGNLPQYES EAIMVAFELN ALLIAEDKDV INKAKELGVN AIPIEELLAS SLEHHHHHH

Formula weight

13012.88 Da

Source organism

Saccharolobus solfataricus P2

Exptl. method

solution NMR

Refine. method

molecular dynamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.8 %, Completeness: 92.4 %, Completeness (bb): 93.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.4 % (1283 of 1388)	92.4 % (661 of 715)	92.2 % (509 of 552)	93.4 % (113 of 121)
Backbone	93.2 % (656 of 704)	92.5 % (222 of 240)	93.7 % (328 of 350)	93.0 % (106 of 114)
Sidechain	90.1 % (717 of 796)	90.3 % (429 of 475)	89.5 % (281 of 314)	100.0 % (7 of 7)
Aromatic	55.8 % (58 of 104)	55.8 % (29 of 52)	55.8 % (29 of 52)
Methyl	100.0 % (160 of 160)	100.0 % (80 of 80)	100.0 % (80 of 80)

1. sso1118

MFAVISPSAF GKLKEILGSN KNYKFVITTL GVSFAIKSGI DIDSALDRGV IVRAFSHKPP KVGNLPQYES EAIMVAFELN ALLIAEDKDV INKAKELGVN AIPIEELLAS SLEHHHHHH

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	sso1118	natural abundance	0.8 (±0.1) mM
2	potassium phosphate	natural abundance	50 mM
3	DSS	natural abundance	0.02 %
4	sodium azide	natural abundance	0.02 %
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.19 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.19 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MDT, Strand ID: A Detail

Release date

2011-01-17

Citation

Resonance assignments of a putative PilT N-terminus domain protein SSO1118 from hyperthermophilic archaeon Sulfolobus solfataricus P2
Xuan, J., Song, X., Wang, J., Feng, Y.
Biomol. NMR Assign. (2011), 5, 161-164, PubMed 21229398 , DOI 10.1007/s12104-010-9291-0 , Abstract

Related entities 1. sso1118, : 1 entities Detail

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC