BMRB: 17303

1H and 15N RESONANCE ASSIGNMENT OF HUMAN HOLO L-FABP

Authors

Cai, J., Luecke, C., Chen, Z., Qiao, Y., Klimtchuk, E.S., Hamilton, J.A.

Assembly

human liver fatty acid binding protein

Entity

1. human liver fatty acid binding protein (polymer, Thiol state: all free), 126 monomers, 14077.01 Da Detail

SFSGKYQLQS QENFEAFMKA IGLPEELIQK GKDIKGVSEI VQNGKHFKFT ITAGSKVIQN EFTVGEECEL ETMTGEKVKT VVQLEGDNKL VTTFKNIKSV TELNGDIITN TMTLGDIVFK RISKRI

Formula weight

14077.01 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 99.0 %, Completeness (bb): 98.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	99.0 % (916 of 925)	99.0 % (778 of 786)	99.3 % (138 of 139)
Backbone	98.4 % (381 of 387)	98.1 % (257 of 262)	99.2 % (124 of 125)
Sidechain	99.4 % (535 of 538)	99.4 % (521 of 524)	100.0 % (14 of 14)
Aromatic	100.0 % (46 of 46)	100.0 % (46 of 46)
Methyl	98.7 % (74 of 75)	98.7 % (74 of 75)

1. L-FABP

SFSGKYQLQS QENFEAFMKA IGLPEELIQK GKDIKGVSEI VQNGKHFKFT ITAGSKVIQN EFTVGEECEL ETMTGEKVKT VVQLEGDNKL VTTFKNIKSV TELNGDIITN TMTLGDIVFK RISKRI

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details non-labeled L-FABP

#	Name	Isotope labeling	Type	Concentration
1	potassium phosphate	natural abundance		20 mM
2	sodium chloride	natural abundance		150 mM
3	sodium azide	natural abundance		0.05 %
4	human liver fatty acid binding protein	natural abundance		3 mM
5	potassium oleate	[U-13C]		15 mM
6	H2O	natural abundance		95 %
7	D2O	natural abundance		5 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 15N-labeled L-FABP

#	Name	Isotope labeling	Type	Concentration
8	potassium phosphate	natural abundance		20 mM
9	sodium chloride	natural abundance		150 mM
10	sodium azide	natural abundance		0.05 %
11	human liver fatty acid binding protein	[U-15N]		2 mM
12	potassium oleate	natural abundance		10 mM
13	H2O	natural abundance		95 %
14	D2O	natural abundance		5 %

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Protein Blocks Logo

Calculated from 10 models in PDB: 2LKK, Strand ID: A Detail

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Release date

2012-06-04

Citation

Solution structure and backbone dynamics of human liver fatty acid binding protein: fatty acid binding revisited
Cai, J., Luecke, C., Chen, Z., Qiao, Y., Klimtchuk, E.S., Hamilton, J.A.
Biophys. J. (2012), 102, 2585-2594, PubMed 22713574 , DOI 10.1016/j.bpj.2012.04.039 , Abstract

Related entities 1. human liver fatty acid binding protein, : 1 : 30 : 1 : 200 entities Detail

Interaction partners 1. human liver fatty acid binding protein, : 2 interactors Detail

Experiments performed 5 experiments Detail