BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	90.6 % (1096 of 1210)	92.6 % (586 of 633)	86.4 % (408 of 472)	97.1 % (102 of 105)
Backbone	95.0 % (576 of 606)	95.7 % (202 of 211)	93.9 % (278 of 296)	97.0 % (96 of 99)
Sidechain	87.6 % (610 of 696)	91.0 % (384 of 422)	82.1 % (220 of 268)	100.0 % (6 of 6)
Aromatic	56.7 % (51 of 90)	95.6 % (43 of 45)	11.9 % (5 of 42)	100.0 % (3 of 3)
Methyl	99.1 % (115 of 116)	100.0 % (58 of 58)	98.3 % (57 of 58)

1. NLRP12 PYD

GHMLRTAGRD GLCRLSTYLE ELEAVELKKF KLYLGTATEL GEGKIPWGSM EKAGPLEMAQ LLITHFGPEE AWRLALSTFE RINRKDLWER GQREDLVRDT VE

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N-labeled NLRP12 PYD sample

#	Name	Isotope labeling	Concentration
1	NLRP12 PYD	[U-99% 15N]	600 uM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	500 mM
4	TCEP	natural abundance	0.5 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 13C/15N-labeled NLRP12 PYD sample

#	Name	Isotope labeling	Concentration
7	NLRP12 PYD	[U-99% 13C; U-99% 15N]	600 uM
8	sodium phosphate	natural abundance	20 mM
9	sodium chloride	natural abundance	500 mM
10	TCEP	natural abundance	0.5 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details Unlabeled NLRP12 PYD sample

#	Name	Isotope labeling	Concentration
13	NLRP12 PYD	natural abundance	600 uM
14	sodium phosphate	natural abundance	20 mM
15	sodium chloride	natural abundance	500 mM
16	TCEP	natural abundance	0.5 mM
17	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.16 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.38 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L6A, Strand ID: A Detail

Release date

2011-12-01

Citation

The NLRP12 pyrin domain: structure, dynamics, and functional insights
Pinheiro, A.S., Eibl, C., Ekman-Vural, Z., Schwarzenbacher, R., Peti, W.
J. Mol. Biol. (2011), 413, 790-803, PubMed 21978668 , DOI 10.1016/j.jmb.2011.09.024 , Abstract

Related entities 1. NLRP12 PYD monomer, : 1 : 4 : 29 entities Detail

Interaction partners 1. NLRP12 PYD monomer, : 24 interactors Detail

More details for 24 interactors identified by 3 citations

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N-labeled NLRP12 PYD sample

#	Name	Isotope labeling	Concentration
1	NLRP12 PYD	[U-99% 15N]	600 uM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	500 mM
4	TCEP	natural abundance	0.5 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 13C/15N-labeled NLRP12 PYD sample

#	Name	Isotope labeling	Concentration
7	NLRP12 PYD	[U-99% 13C; U-99% 15N]	600 uM
8	sodium phosphate	natural abundance	20 mM
9	sodium chloride	natural abundance	500 mM
10	TCEP	natural abundance	0.5 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

3 3D HNCA

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 13C/15N-labeled NLRP12 PYD sample

#	Name	Isotope labeling	Concentration
7	NLRP12 PYD	[U-99% 13C; U-99% 15N]	600 uM
8	sodium phosphate	natural abundance	20 mM
9	sodium chloride	natural abundance	500 mM
10	TCEP	natural abundance	0.5 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

4 3D HNCACB

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 13C/15N-labeled NLRP12 PYD sample

#	Name	Isotope labeling	Concentration
7	NLRP12 PYD	[U-99% 13C; U-99% 15N]	600 uM
8	sodium phosphate	natural abundance	20 mM
9	sodium chloride	natural abundance	500 mM
10	TCEP	natural abundance	0.5 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

5 3D CBCA(CO)NH

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 13C/15N-labeled NLRP12 PYD sample

#	Name	Isotope labeling	Concentration
7	NLRP12 PYD	[U-99% 13C; U-99% 15N]	600 uM
8	sodium phosphate	natural abundance	20 mM
9	sodium chloride	natural abundance	500 mM
10	TCEP	natural abundance	0.5 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

6 3D HNCO

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 13C/15N-labeled NLRP12 PYD sample

#	Name	Isotope labeling	Concentration
7	NLRP12 PYD	[U-99% 13C; U-99% 15N]	600 uM
8	sodium phosphate	natural abundance	20 mM
9	sodium chloride	natural abundance	500 mM
10	TCEP	natural abundance	0.5 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

7 3D HN(CA)CO

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 13C/15N-labeled NLRP12 PYD sample

#	Name	Isotope labeling	Concentration
7	NLRP12 PYD	[U-99% 13C; U-99% 15N]	600 uM
8	sodium phosphate	natural abundance	20 mM
9	sodium chloride	natural abundance	500 mM
10	TCEP	natural abundance	0.5 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

8 3D HBHA(CO)NH

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 13C/15N-labeled NLRP12 PYD sample

#	Name	Isotope labeling	Concentration
7	NLRP12 PYD	[U-99% 13C; U-99% 15N]	600 uM
8	sodium phosphate	natural abundance	20 mM
9	sodium chloride	natural abundance	500 mM
10	TCEP	natural abundance	0.5 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

9 3D C(CO)NH

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 13C/15N-labeled NLRP12 PYD sample

#	Name	Isotope labeling	Concentration
7	NLRP12 PYD	[U-99% 13C; U-99% 15N]	600 uM
8	sodium phosphate	natural abundance	20 mM
9	sodium chloride	natural abundance	500 mM
10	TCEP	natural abundance	0.5 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

10 3D HCCH-TOCSY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 13C/15N-labeled NLRP12 PYD sample

#	Name	Isotope labeling	Concentration
7	NLRP12 PYD	[U-99% 13C; U-99% 15N]	600 uM
8	sodium phosphate	natural abundance	20 mM
9	sodium chloride	natural abundance	500 mM
10	TCEP	natural abundance	0.5 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

11 3D 1H-15N NOESY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N-labeled NLRP12 PYD sample

#	Name	Isotope labeling	Concentration
1	NLRP12 PYD	[U-99% 15N]	600 uM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	500 mM
4	TCEP	natural abundance	0.5 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

12 3D 1H-13C NOESY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 13C/15N-labeled NLRP12 PYD sample

#	Name	Isotope labeling	Concentration
7	NLRP12 PYD	[U-99% 13C; U-99% 15N]	600 uM
8	sodium phosphate	natural abundance	20 mM
9	sodium chloride	natural abundance	500 mM
10	TCEP	natural abundance	0.5 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

13 2D 1H-1H NOESY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details Unlabeled NLRP12 PYD sample

#	Name	Isotope labeling	Concentration
13	NLRP12 PYD	natural abundance	600 uM
14	sodium phosphate	natural abundance	20 mM
15	sodium chloride	natural abundance	500 mM
16	TCEP	natural abundance	0.5 mM
17	D2O	natural abundance	100 %

14 2D 1H-1H TOCSY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details Unlabeled NLRP12 PYD sample

#	Name	Isotope labeling	Concentration
13	NLRP12 PYD	natural abundance	600 uM
14	sodium phosphate	natural abundance	20 mM
15	sodium chloride	natural abundance	500 mM
16	TCEP	natural abundance	0.5 mM
17	D2O	natural abundance	100 %

15 2D 1H-1H COSY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details Unlabeled NLRP12 PYD sample

#	Name	Isotope labeling	Concentration
13	NLRP12 PYD	natural abundance	600 uM
14	sodium phosphate	natural abundance	20 mM
15	sodium chloride	natural abundance	500 mM
16	TCEP	natural abundance	0.5 mM
17	D2O	natural abundance	100 %

nullKeywords apoptosis, innate immunity, NLRP12 PYD, NMR, resonance assignment, death domain, NLRP12, PYRIN

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BMRB: 17305

Sample #1

Sample #2

Sample #3

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. NLRP12 PYD monomer, : 1 : 4 : 29 entities Detail

Interaction partners 1. NLRP12 PYD monomer, : 24 interactors Detail

Experiments performed 15 experiments Detail

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