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In response to the declaration of global pandemic of Coronavirus infectious disease 2019 (COVID-19) caused by Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), we provide comprehensive content on Coronavirus molecular structures and its interactions:

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BMRB: 17309

2L6I

Solution structure of coronaviral stem-loop 2 (SL2)

Authors

Lee, C., Li, L., Giedroc, D.P.

Assembly

coronavirus stem-loop 2

Entity

1. coronavirus stem-loop 2 (polymer, Thiol state: not present), 16 monomers, 5122.995 Da Detail

GAUCUCUUGU AGAUCA

Formula weight

5122.995 Da

Source organism

Alphacoronavirus

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 60.4 %, Completeness (bb): 58.9 % Detail

Polymer type: polyribonucleotide

	Total	¹H	¹³C	³¹P
All	60.4 % (160 of 265)	86.4 % (121 of 140)	32.1 % (35 of 109)	25.0 % (4 of 16)
Suger, PO4	58.9 % (113 of 192)	83.3 % (80 of 96)	36.3 % (29 of 80)	25.0 % (4 of 16)
Nucleobase	64.4 % (47 of 73)	93.2 % (41 of 44)	20.7 % (6 of 29)
Aromatic	61.2 % (41 of 67)	92.1 % (35 of 38)	20.7 % (6 of 29)

1. SL2

GAUCUCUUGU AGAUCA

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	SL2	natural abundance		0.8 ~ 2.0 mM
2	potassium phosphate	natural abundance		10 mM
3	DSS	natural abundance		0.1 mM
4	D2O	natural abundance		100 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
5	SL2	[U-13C; U-15N]-Ura		0.8 ~ 2.0 mM
6	potassium phosphate	natural abundance		10 mM
7	DSS	natural abundance		0.1 mM
8	D2O	natural abundance		10 %
9	H2O	natural abundance		90 %

Release date

2010-12-01

Citation

The solution structure of coronaviral stem-loop 2 (SL2) reveals a canonical CUYG tetraloop fold
Lee, C., Li, L., Giedroc, D.P.
FEBS Lett. (2011), 585, 1049-1053, PubMed 21382373 , DOI 10.1016/j.febslet.2011.03.002 , Abstract

Related entities 1. coronavirus stem-loop 2, : 1 entities Detail

Experiments performed 9 experiments Detail

1 2D 1H-1H NOESY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	SL2	natural abundance		0.8 ~ 2.0 mM
2	potassium phosphate	natural abundance		10 mM
3	DSS	natural abundance		0.1 mM
4	D2O	natural abundance		100 %

2 2D 1H-1H TOCSY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	SL2	natural abundance		0.8 ~ 2.0 mM
2	potassium phosphate	natural abundance		10 mM
3	DSS	natural abundance		0.1 mM
4	D2O	natural abundance		100 %

3 2D 1H-13C HSQC

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	SL2	natural abundance		0.8 ~ 2.0 mM
2	potassium phosphate	natural abundance		10 mM
3	DSS	natural abundance		0.1 mM
4	D2O	natural abundance		100 %

4 3D HCCH-TOCSY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	SL2	natural abundance		0.8 ~ 2.0 mM
2	potassium phosphate	natural abundance		10 mM
3	DSS	natural abundance		0.1 mM
4	D2O	natural abundance		100 %

5 2D HNCCCH

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	SL2	natural abundance		0.8 ~ 2.0 mM
2	potassium phosphate	natural abundance		10 mM
3	DSS	natural abundance		0.1 mM
4	D2O	natural abundance		100 %

6 2D H(CCN)H-TOCSY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	SL2	natural abundance		0.8 ~ 2.0 mM
2	potassium phosphate	natural abundance		10 mM
3	DSS	natural abundance		0.1 mM
4	D2O	natural abundance		100 %

7 2D HCN-HMQC

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	SL2	natural abundance		0.8 ~ 2.0 mM
2	potassium phosphate	natural abundance		10 mM
3	DSS	natural abundance		0.1 mM
4	D2O	natural abundance		100 %

8 2D HCN-TROSY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	SL2	natural abundance		0.8 ~ 2.0 mM
2	potassium phosphate	natural abundance		10 mM
3	DSS	natural abundance		0.1 mM
4	D2O	natural abundance		100 %

9 2D HCNCH

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	SL2	natural abundance		0.8 ~ 2.0 mM
2	potassium phosphate	natural abundance		10 mM
3	DSS	natural abundance		0.1 mM
4	D2O	natural abundance		100 %

nullKeywords coronavirus, mouse hepatitis virus, RNA tetraloop, severe acute respiratory syndrome