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BMRB: 17314

2L6K

Solution Structure of a Nonphosphorylated Peptide Recognizing Domain

Authors

Dai, K., Liao, S., Zhang, J., Zhang, X., Tu, X.

Assembly

Nonphosphorylated Peptide Recognizing Domain

Entity

1. Nonphosphorylated Peptide Recognizing Domain (polymer, Thiol state: all free), 123 monomers, 13663.58 Da Detail

MDTSKFWYKP HLSRDQAIAL LKDKDPGAFL IRDSHSFQGA YGLALKVATP PPSAQPWKGD PVEQLVRHFL IETGPKGVKI KGCPSEPYFG SLSALVSQHS ISPISLPCCL RIPSKLEHHH HHH

Formula weight

13663.58 Da

Source organism

Exptl. method

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.9 %, Completeness: 73.7 %, Completeness (bb): 86.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	73.7 % (1071 of 1453)	76.6 % (582 of 760)	67.9 % (391 of 576)	83.8 % (98 of 117)
Backbone	86.7 % (617 of 712)	88.0 % (212 of 241)	85.3 % (308 of 361)	88.2 % (97 of 110)
Sidechain	64.5 % (552 of 856)	71.3 % (370 of 519)	54.8 % (181 of 330)	14.3 % (1 of 7)
Aromatic	11.6 % (16 of 138)	18.8 % (13 of 69)	4.5 % (3 of 67)	0.0 % (0 of 2)
Methyl	77.9 % (95 of 122)	95.1 % (58 of 61)	60.7 % (37 of 61)

1. Protein Domain

MDTSKFWYKP HLSRDQAIAL LKDKDPGAFL IRDSHSFQGA YGLALKVATP PPSAQPWKGD PVEQLVRHFL IETGPKGVKI KGCPSEPYFG SLSALVSQHS ISPISLPCCL RIPSKLEHHH HHH

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	Protein Domain	[U-99% 13C; U-99% 15N]		0.6 mM
2	sodium chloride	natural abundance		150 mM
3	sodium phosphate	natural abundance		20 mM
4	EDTA	natural abundance		2 mM
5	Arginine	natural abundance		50 mM
6	glutamine	natural abundance		50 mM
7	H2O	natural abundance		90 %
8	D2O	natural abundance		10 %

LACS Plot; CA

Referencing offset: -0.05 ppm, Outliers: 4 Detail

LACS Plot; CB

Referencing offset: -0.05 ppm, Outliers: 4 Detail

LACS Plot; HA

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Referencing offset: 0.09 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L6K, Strand ID: A Detail

Release date

2012-07-24

Citation

Solution Structure of a Nonphosphorylated Peptide Recognizing Domain
Dai, K., Liao, S., Zhang, J., Zhang, X., Tu, X.
Not known

Related entities 1. Nonphosphorylated Peptide Recognizing Domain, : 1 : 3 : 200 entities Detail

Interaction partners 1. Nonphosphorylated Peptide Recognizing Domain, : 35 interactors Detail

More details for 35 interactors identified by 10 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	Protein Domain	[U-99% 13C; U-99% 15N]		0.6 mM
2	sodium chloride	natural abundance		150 mM
3	sodium phosphate	natural abundance		20 mM
4	EDTA	natural abundance		2 mM
5	Arginine	natural abundance		50 mM
6	glutamine	natural abundance		50 mM
7	H2O	natural abundance		90 %
8	D2O	natural abundance		10 %

2 3D CBCA(CO)NH

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	Protein Domain	[U-99% 13C; U-99% 15N]		0.6 mM
2	sodium chloride	natural abundance		150 mM
3	sodium phosphate	natural abundance		20 mM
4	EDTA	natural abundance		2 mM
5	Arginine	natural abundance		50 mM
6	glutamine	natural abundance		50 mM
7	H2O	natural abundance		90 %
8	D2O	natural abundance		10 %

3 3D HNCACB

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	Protein Domain	[U-99% 13C; U-99% 15N]		0.6 mM
2	sodium chloride	natural abundance		150 mM
3	sodium phosphate	natural abundance		20 mM
4	EDTA	natural abundance		2 mM
5	Arginine	natural abundance		50 mM
6	glutamine	natural abundance		50 mM
7	H2O	natural abundance		90 %
8	D2O	natural abundance		10 %

4 3D C(CO)NH

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	Protein Domain	[U-99% 13C; U-99% 15N]		0.6 mM
2	sodium chloride	natural abundance		150 mM
3	sodium phosphate	natural abundance		20 mM
4	EDTA	natural abundance		2 mM
5	Arginine	natural abundance		50 mM
6	glutamine	natural abundance		50 mM
7	H2O	natural abundance		90 %
8	D2O	natural abundance		10 %

5 3D HBHA(CO)NH

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	Protein Domain	[U-99% 13C; U-99% 15N]		0.6 mM
2	sodium chloride	natural abundance		150 mM
3	sodium phosphate	natural abundance		20 mM
4	EDTA	natural abundance		2 mM
5	Arginine	natural abundance		50 mM
6	glutamine	natural abundance		50 mM
7	H2O	natural abundance		90 %
8	D2O	natural abundance		10 %

6 3D H(CCO)NH

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	Protein Domain	[U-99% 13C; U-99% 15N]		0.6 mM
2	sodium chloride	natural abundance		150 mM
3	sodium phosphate	natural abundance		20 mM
4	EDTA	natural abundance		2 mM
5	Arginine	natural abundance		50 mM
6	glutamine	natural abundance		50 mM
7	H2O	natural abundance		90 %
8	D2O	natural abundance		10 %

7 3D HN(CO)CA

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	Protein Domain	[U-99% 13C; U-99% 15N]		0.6 mM
2	sodium chloride	natural abundance		150 mM
3	sodium phosphate	natural abundance		20 mM
4	EDTA	natural abundance		2 mM
5	Arginine	natural abundance		50 mM
6	glutamine	natural abundance		50 mM
7	H2O	natural abundance		90 %
8	D2O	natural abundance		10 %

8 3D 1H-15N NOESY

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	Protein Domain	[U-99% 13C; U-99% 15N]		0.6 mM
2	sodium chloride	natural abundance		150 mM
3	sodium phosphate	natural abundance		20 mM
4	EDTA	natural abundance		2 mM
5	Arginine	natural abundance		50 mM
6	glutamine	natural abundance		50 mM
7	H2O	natural abundance		90 %
8	D2O	natural abundance		10 %

9 3D 1H-13C NOESY

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	Protein Domain	[U-99% 13C; U-99% 15N]		0.6 mM
2	sodium chloride	natural abundance		150 mM
3	sodium phosphate	natural abundance		20 mM
4	EDTA	natural abundance		2 mM
5	Arginine	natural abundance		50 mM
6	glutamine	natural abundance		50 mM
7	H2O	natural abundance		90 %
8	D2O	natural abundance		10 %

10 3D HCCH-COSY

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	Protein Domain	[U-99% 13C; U-99% 15N]		0.6 mM
2	sodium chloride	natural abundance		150 mM
3	sodium phosphate	natural abundance		20 mM
4	EDTA	natural abundance		2 mM
5	Arginine	natural abundance		50 mM
6	glutamine	natural abundance		50 mM
7	H2O	natural abundance		90 %
8	D2O	natural abundance		10 %

nullKeywords protein