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BMRB: 17316

2KZL

Specifier domain and GA motif region of B. subtilis tyrS T box leader RNA

Authors

Wang, J.

Assembly

T box riboswitch Specifier domain and GA motif

Entity

1. T box riboswitch Specifier domain and GA motif (polymer, Thiol state: not present), 55 monomers, 17806.60 Da Detail

GGGAGUAAAG AUUGAGACAA GUAGGACUUC GGUCCGAAUA CACUCAUGAA CUCCC

Formula weight

17806.6 Da

Source organism

Bacillus subtilis

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 88.6 %, Completeness (bb): 89.9 % Detail

Polymer type: polyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	88.6 % (772 of 871)	85.9 % (408 of 475)	93.3 % (346 of 371)	72.0 % (18 of 25)
Suger, PO4	89.9 % (544 of 605)	89.1 % (294 of 330)	90.9 % (250 of 275)
Nucleobase	85.7 % (228 of 266)	78.6 % (114 of 145)	100.0 % (96 of 96)	72.0 % (18 of 25)
Aromatic	94.2 % (228 of 242)	94.2 % (114 of 121)	100.0 % (96 of 96)	72.0 % (18 of 25)

1. Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA

GGGAGUAAAG AUUGAGACAA GUAGGACUUC GGUCCGAAUA CACUCAUGAA CUCCC

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA	[U-100% 13C; U-100% 15N]		1 mM
2	D2O	natural abundance		100 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Concentration
3	Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA	[U-100% 13C; U-100% 15N]	1 mM
4	D2O	natural abundance	10 %
5	H2O	natural abundance	90 %

Release date

2011-02-21

Citation

Solution structure of the K-turn and Specifier Loop domains from the Bacillus subtilis tyrS T-box leader RNA
Wang, J., Nikonowicz, E.P.
J. Mol. Biol. (2011), 408, 99-117, PubMed 21333656 , DOI 10.1016/j.jmb.2011.02.014 , Abstract

Related entities 1. T box riboswitch Specifier domain and GA motif, : 1 entities Detail

Experiments performed 12 experiments Detail

1 2D 1H-13C HSQC

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA	[U-100% 13C; U-100% 15N]		1 mM
2	D2O	natural abundance		100 %

2 3D HCCH-TOCSY

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA	[U-100% 13C; U-100% 15N]		1 mM
2	D2O	natural abundance		100 %

3 2D 1H-1H NOESY

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA	[U-100% 13C; U-100% 15N]		1 mM
2	D2O	natural abundance		100 %

4 3D 1H-13C NOESY

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA	[U-100% 13C; U-100% 15N]		1 mM
2	D2O	natural abundance		100 %

5 2D 1H-15N HSQC

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Concentration
3	Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA	[U-100% 13C; U-100% 15N]	1 mM
4	D2O	natural abundance	10 %
5	H2O	natural abundance	90 %

6 3D 1H-15N NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Concentration
3	Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA	[U-100% 13C; U-100% 15N]	1 mM
4	D2O	natural abundance	10 %
5	H2O	natural abundance	90 %

7 2D 1H-13C HSQC

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Concentration
3	Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA	[U-100% 13C; U-100% 15N]	1 mM
4	D2O	natural abundance	10 %
5	H2O	natural abundance	90 %

8 3D HCCH-COSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA	[U-100% 13C; U-100% 15N]		1 mM
2	D2O	natural abundance		100 %

9 3D HCN

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA	[U-100% 13C; U-100% 15N]		1 mM
2	D2O	natural abundance		100 %

10 2D 1H-1H NOESY

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Concentration
3	Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA	[U-100% 13C; U-100% 15N]	1 mM
4	D2O	natural abundance	10 %
5	H2O	natural abundance	90 %

11 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA	[U-100% 13C; U-100% 15N]		1 mM
2	D2O	natural abundance		100 %

12 2D 1H-13C ct-spin-echo difference

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA	[U-100% 13C; U-100% 15N]		1 mM
2	D2O	natural abundance		100 %

nullKeywords GA motif, Kink turn, K-turn, loop E motif, Specifier Domain, T box riboswitch

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Found 1 Document (0.584 seconds)

BMRB: 17316

Sample #1

Sample #2

Related entities 1. T box riboswitch Specifier domain and GA motif, : 1 entities Detail

Experiments performed 12 experiments Detail

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