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BMRB: 17321

2L6Q

New high resolution NMR structure of gpW (W protein of bacteriophage lambda) at neutral pH

Authors

Sborgi, L., Verma, A., Munoz, V., de Alba, E.

Assembly

W protein of bacteriophage lambda

Entity

1. W protein of bacteriophage lambda (polymer, Thiol state: not present), 62 monomers, 6975.946 Da Detail

MVRQEELAAA RAALHDLMTG KRVATVQKDG RRVEFTATSV SDLKKYIAEL EVQTGMTQRR RG

Formula weight

6975.946 Da

Source organism

Escherichia coli

Exptl. method

Refine. method

distance geometry, DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.4 %, Completeness: 87.1 %, Completeness (bb): 84.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	87.1 % (617 of 708)	93.2 % (345 of 370)	77.6 % (211 of 272)	92.4 % (61 of 66)
Backbone	84.1 % (313 of 372)	98.4 % (126 of 128)	69.2 % (126 of 182)	98.4 % (61 of 62)
Sidechain	91.6 % (361 of 394)	90.5 % (219 of 242)	95.9 % (142 of 148)	0.0 % (0 of 4)
Aromatic	90.9 % (20 of 22)	90.9 % (10 of 11)	90.9 % (10 of 11)
Methyl	100.0 % (76 of 76)	100.0 % (38 of 38)	100.0 % (38 of 38)

1. gpW

MVRQEELAAA RAALHDLMTG KRVATVQKDG RRVEFTATSV SDLKKYIAEL EVQTGMTQRR RG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 294 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	gpW	[U-13C; U-15N]		1 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %
4	phosphate buffer	natural abundance		20 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 294 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
5	gpW	[U-13C; U-15N]		1 mM
6	D2O	natural abundance		100 %
7	phosphate buffer	natural abundance		20 mM

LACS Plot; CA

Referencing offset: 0.18 ppm, Outliers: 1 Detail

LACS Plot; CB

Referencing offset: 0.18 ppm, Outliers: 1 Detail

LACS Plot; HA

Referencing offset: -0.11 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L6Q, Strand ID: A Detail

Release date

2011-11-06

Citation

Revisiting the NMR structure of the ultrafast downhill folding protein gpW from bacteriophage λ
Sborgi, L., Verma, A., Munoz, V., de Alba, E.
PLoS One (2011), 6, e26409-e26409, PubMed 22087227 , DOI 10.1371/journal.pone.0026409 , Abstract

Related entities 1. W protein of bacteriophage lambda, : 1 : 1 : 6 : 7 entities Detail

Experiments performed 8 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 294 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	gpW	[U-13C; U-15N]		1 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %
4	phosphate buffer	natural abundance		20 mM

2 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 294 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	gpW	[U-13C; U-15N]		1 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %
4	phosphate buffer	natural abundance		20 mM

3 3D HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 294 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	gpW	[U-13C; U-15N]		1 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %
4	phosphate buffer	natural abundance		20 mM

4 3D C(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 294 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	gpW	[U-13C; U-15N]		1 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %
4	phosphate buffer	natural abundance		20 mM

5 2D 1H-1H TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 294 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	gpW	[U-13C; U-15N]		1 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %
4	phosphate buffer	natural abundance		20 mM

6 3D HCCH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 294 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	gpW	[U-13C; U-15N]		1 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %
4	phosphate buffer	natural abundance		20 mM

7 3D 1H-15N NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 294 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	gpW	[U-13C; U-15N]		1 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %
4	phosphate buffer	natural abundance		20 mM

8 4D 1H-13C NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 294 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
5	gpW	[U-13C; U-15N]		1 mM
6	D2O	natural abundance		100 %
7	phosphate buffer	natural abundance		20 mM

nullKeywords downhill protein folding, fast protein folding, folding simulations, gpW, NMR