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BMRB: 17323

2L6U

Solution NMR Structure of Med25(391-543) Comprising the Activator-Interacting Domain (ACID) of Human Mediator Subuniti 25. Northeast Structural Genomics Consortium Target HR6188A

Authors

Eletsky, A., Ryuechan, W.T., Sukumaran, D.K., Shastry, R., Ciccosanti, C., Janjua, H., Acton, T.B., Xiao, R., Everett, J.K., Montelione, G.T., Szyperski, T.

Assembly

HR6188A

Entity

1. HR6188A (polymer, Thiol state: all free), 163 monomers, 18609.37 Da Detail

MGHHHHHHSH GQQSVSNKLL AWSGVLEWQE KPKPASVDAN TKLTRSLPCQ VYVNHGENLK TEQWPQKLIM QLIPQQLLTT LGPLFRNSRM VQFHFTNKDL ESLKGLYRIM GNGFAGCVHF PHTAPCEVRV LMLLYSSKKK IFMGLIPYDQ SGFVNGIRQV ITN

Formula weight

18609.37 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.3 %, Completeness: 95.6 %, Completeness (bb): 95.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	95.6 % (1890 of 1978)	95.6 % (988 of 1034)	95.7 % (733 of 766)	94.9 % (169 of 178)
Backbone	95.3 % (915 of 960)	94.8 % (312 of 329)	96.0 % (458 of 477)	94.2 % (145 of 154)
Sidechain	95.1 % (1112 of 1169)	95.0 % (670 of 705)	95.0 % (418 of 440)	100.0 % (24 of 24)
Aromatic	87.4 % (159 of 182)	87.9 % (80 of 91)	86.4 % (76 of 88)	100.0 % (3 of 3)
Methyl	100.0 % (174 of 174)	100.0 % (87 of 87)	100.0 % (87 of 87)

1. HR6188A

MGHHHHHHSH GQQSVSNKLL AWSGVLEWQE KPKPASVDAN TKLTRSLPCQ VYVNHGENLK TEQWPQKLIM QLIPQQLLTT LGPLFRNSRM VQFHFTNKDL ESLKGLYRIM GNGFAGCVHF PHTAPCEVRV LMLLYSSKKK IFMGLIPYDQ SGFVNGIRQV ITN

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.7 mM [U-100% 13C; U-100% 15N] HR6188A, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	HR6188A	[U-100% 13C; U-100% 15N]	0.7 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	sodium azide	natural abundance	0.02 %
7	D2O	natural abundance	10 %
8	H2O	natural abundance	90 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.4 mM [U-5% 13C; U-100% 15N] HR6188A, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
9	HR6188A	[U-5% 13C; U-100% 15N]	0.4 mM
10	MES	natural abundance	20 mM
11	sodium chloride	natural abundance	100 mM
12	calcium chloride	natural abundance	5 mM
13	DTT	natural abundance	10 mM
14	sodium azide	natural abundance	0.02 %
15	D2O	natural abundance	10 %
16	H2O	natural abundance	90 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.17 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L6U, Strand ID: A Detail

Release date

2010-12-21

Citation

Solution NMR structure of MED25(391-543) comprising the activator-interacting domain (ACID) of human mediator subunit 25
Eletsky, A., Ruyechan, W.T., Xiao, R., Acton, T.B., Montelione, G.T., Szyperski, T.
J. Struct. Funct. Genomics (2011), 12, 159-166, PubMed 21785987 , DOI 10.1007/s10969-011-9115-1 , Abstract

Related entities 1. HR6188A, : 1 : 10 : 25 entities Detail

Interaction partners 1. HR6188A, : 4 interactors Detail

More details for 4 interactors identified by 2 citations

Experiments performed 19 experiments Detail

1 2D 1H-15N HSQC