BMRB: 17327

Not known

Authors

Reckel, S., Gottstein, D., Stehle, J., Loehr, F., Takeda, M., Silvers, R., Kainosho, M., Glaubitz, C., Bernhard, F., Schwalbe, H., Guntert, P., Doetsch, V.

Assembly

proteorhodopsin

Entity

1. proteorhodopsin (polymer, Thiol state: all free), 243 monomers, 26975.23 Da Detail

MGGGDLDASD YTGVSFWLVT AALLASTVFF FVERDRVSAK WKTSLTVSGL VTGIAFWHYM YMRGVWIETG DSPTVFRYID WLLTVPLLIC EFYLILAAAT NVAGSLFKKL LVGSLVMLVF GYMGEAGIMA AWPAFIIGCL AWVYMIYELW AGEGKSACNT ASPAVQSAYN TMMYIIIFGW AIYPVGYFTG YLMGDGGSAL NLNLIYNLAD FVNXILFGLI IWNVAVKESS NAPGGGSHHH HHH

Formula weight

26975.23 Da

Source organism

uncultured Gammaproteobacteria bacterium

Exptl. method

solution NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.5 %, Completeness: 67.2 %, Completeness (bb): 87.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	67.2 % (1882 of 2800)	58.7 % (825 of 1406)	72.9 % (830 of 1138)	88.7 % (227 of 256)
Backbone	87.9 % (1266 of 1440)	82.9 % (418 of 504)	89.9 % (629 of 700)	92.8 % (219 of 236)
Sidechain	50.7 % (799 of 1576)	44.9 % (405 of 902)	59.0 % (386 of 654)	40.0 % (8 of 20)
Aromatic	29.2 % (117 of 400)	29.0 % (58 of 200)	26.8 % (51 of 190)	80.0 % (8 of 10)
Methyl	86.0 % (282 of 328)	86.0 % (141 of 164)	86.0 % (141 of 164)

1. proteorhodopsin

MGGGDLDASD YTGVSFWLVT AALLASTVFF FVERDRVSAK WKTSLTVSGL VTGIAFWHYM YMRGVWIETG DSPTVFRYID WLLTVPLLIC EFYLILAAAT NVAGSLFKKL LVGSLVMLVF GYMGEAGIMA AWPAFIIGCL AWVYMIYELW AGEGKSACNT ASPAVQSAYN TMMYIIIFGW AIYPVGYFTG YLMGDGGSAL NLNLIYNLAD FVNXILFGLI IWNVAVKESS NAPGGGSHHH HHH

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 K, pH 5

#	Name	Isotope labeling	Type	Concentration
1	proteorhodopsin	[U-13C; U-15N]		300 uM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %
4	NaOAc	natural abundance		25 mM
5	DTT	natural abundance		2 mM
6	diC7PC	natural abundance		2 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 K, pH 5

#	Name	Isotope labeling	Type	Concentration
7	proteorhodopsin	[U-13C; U-15N; U-2H]		300 uM
8	H2O	natural abundance		90 %
9	D2O	natural abundance		10 %
10	NaOAc	natural abundance		25 mM
11	DTT	natural abundance		2 mM
12	diC7PC	natural abundance		2 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 K, pH 5

#	Name	Isotope labeling	Type	Concentration
13	proteorhodopsin	[U-15N; U-2H]		300 uM
14	H2O	natural abundance		90 %
15	D2O	natural abundance		10 %
16	NaOAc	natural abundance		25 mM
17	DTT	natural abundance		2 mM
18	diC7PC	natural abundance		2 %

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Calculated from 20 models in PDB: 2L6X, Strand ID: A Detail

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Release date

2011-10-30

Citation

Solution NMR structure of proteorhodopsin
Reckel, S., Gottstein, D., Stehle, J., Lohr, F., Verhoefen, M., Takeda, M., Silvers, R., Kainosho, M., Glaubitz, C., Wachtveitl, J., Bernhard, F., Schwalbe, H., Guntert, P., Dotsch, V.
Angew. Chem Int Ed Engl. (2011), 50, 11942-11946, PubMed 22034093 , DOI 10.1002/anie.201105648 , Abstract

Related entities 1. proteorhodopsin, : 1 : 3 : 103 entities Detail

Experiments performed 7 experiments Detail