The Solution Structure of Calcium Bound CIB1
MGHHHHHHHH HHSSGHIDDD DKHMGGSGSR LSKELLAEYQ DLTFLTKQEI LLAHRRFCEL LPQEQRSVES SLRAQVPFEQ ILSLPELKAN PFKERICRVF STSPAKDSLS FEDFLDLLSV FSDTATPDIK SHYAFRIFDF DDDGTLNRED LSRLVNCLTG EGEDTRLSAS EMKQLIDNIL EESDIDRDGT INLSEFQHVI SRSPDFASSF KIVL
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 45.5 % (1133 of 2488) | 25.5 % (330 of 1292) | 65.1 % (635 of 976) | 76.4 % (168 of 220) |
Backbone | 71.2 % (904 of 1270) | 45.3 % (195 of 430) | 85.5 % (541 of 633) | 81.2 % (168 of 207) |
Sidechain | 28.4 % (404 of 1423) | 15.5 % (134 of 862) | 49.3 % (270 of 548) | 0.0 % (0 of 13) |
Aromatic | 0.0 % (0 of 216) | 0.0 % (0 of 108) | 0.0 % (0 of 108) | |
Methyl | 77.8 % (168 of 216) | 75.0 % (81 of 108) | 80.6 % (87 of 108) |
1. CIB1
MGHHHHHHHH HHSSGHIDDD DKHMGGSGSR LSKELLAEYQ DLTFLTKQEI LLAHRRFCEL LPQEQRSVES SLRAQVPFEQ ILSLPELKAN PFKERICRVF STSPAKDSLS FEDFLDLLSV FSDTATPDIK SHYAFRIFDF DDDGTLNRED LSRLVNCLTG EGEDTRLSAS EMKQLIDNIL EESDIDRDGT INLSEFQHVI SRSPDFASSF KIVLSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | CIB1 | [U-13C; U-15N; U-2H] | 0.5 mM | |
2 | HEPES | natural abundance | 50 mM | |
3 | potassium chloride | natural abundance | 100 mM | |
4 | calcium cloride | natural abundance | 4 mM | |
5 | DTT | natural abundance | 10 mM | |
6 | Pf1 phage | natural abundance | 12 mg/mL | |
7 | H2O | natural abundance | 90 % | |
8 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | CIB1 | I/L/V methyl 13C and 1H, [U,2H] | 0.5 mM | |
10 | HEPES | natural abundance | 50 mM | |
11 | potassium chloride | natural abundance | 100 mM | |
12 | calcium chloride | natural abundance | 4 mM | |
13 | DTT | natural abundance | 10 mM | |
14 | H2O | natural abundance | 90 % | |
15 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
16 | CIB1 | [U-13C; U-15N; U-2H] | 0.5 mM | |
17 | HEPES | natural abundance | 50 mM | |
18 | potassium chloride | natural abundance | 100 mM | |
19 | calcium cloride | natural abundance | 4 mM | |
20 | DTT | natural abundance | 10 mM | |
21 | H2O | natural abundance | 90 % | |
22 | D2O | natural abundance | 10 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
23 | CIB1 | [U-13C; U-2H]-I/L/V methyl | 0.5 mM | |
24 | HEPES | natural abundance | 50 mM | |
25 | potassium chloride | natural abundance | 100 mM | |
26 | calcium cloride | natural abundance | 4 mM | |
27 | DTT | natural abundance | 10 mM | |
28 | D2O | natural abundance | 100 % |