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Found 1 Document (0.919 seconds)

BMRB: 17343

2L73

Structure of the NOXO1b PX domain

Authors

Davis, N.Y., McPhail, L.C., Horita, D.A.

Assembly

NOXO1b

Entity

1. NOXO1b (polymer, Thiol state: not present), 149 monomers, 16579.98 Da Detail

GPLGSMAGPR YPVSVQGAAL VQIKRLQTFA FSVRWSDGSD TFVRRSWDEF RQLKKTLKET FPVEAGLLRR SDRVLPKLLD APLLGRVGRT SRGLARLQLL ETYSRRLLAT AERVARSPTI TGFFAPQPLD LEPALPPGSR VILPTPEEQ

Formula weight

16579.98 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.6 %, Completeness: 47.6 %, Completeness (bb): 74.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	47.6 % (836 of 1756)	26.8 % (247 of 922)	65.8 % (454 of 690)	93.8 % (135 of 144)
Backbone	74.4 % (644 of 866)	46.8 % (138 of 295)	86.9 % (379 of 436)	94.1 % (127 of 135)
Sidechain	29.5 % (303 of 1028)	17.2 % (108 of 627)	47.7 % (187 of 392)	88.9 % (8 of 9)
Aromatic	1.8 % (2 of 110)	1.8 % (1 of 55)	0.0 % (0 of 53)	50.0 % (1 of 2)
Methyl	77.2 % (142 of 184)	72.8 % (67 of 92)	81.5 % (75 of 92)

1. NOXO1b

GPLGSMAGPR YPVSVQGAAL VQIKRLQTFA FSVRWSDGSD TFVRRSWDEF RQLKKTLKET FPVEAGLLRR SDRVLPKLLD APLLGRVGRT SRGLARLQLL ETYSRRLLAT AERVARSPTI TGFFAPQPLD LEPALPPGSR VILPTPEEQ

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	NOXO1b	[U-13C; U-15N; U-2H]	0.5 (±0.05) mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	0.0 ppm	external	direct	1.0
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

Referencing offset: 0.84 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	0.0 ppm	external	direct	1.0
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

Referencing offset: 0.84 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	0.0 ppm	external	direct	1.0
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

Referencing offset: 0.36 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 14 models in PDB: 2L73, Strand ID: A Detail

Release date

2011-03-29

Citation

Backbone 1H, 15N, and 13C resonance assignments for the NOXO1β PX domain
Davis, N.Y., McPhail, L.C., Horita, D.A.
Biomol. NMR Assign. (2011), 5, 139-141, PubMed 21188560 , DOI 10.1007/s12104-010-9286-x , Abstract

Related entities 1. NOXO1b, : 1 : 15 entities Detail

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	NOXO1b	[U-13C; U-15N; U-2H]	0.5 (±0.05) mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

2 2D IPAP

Instrument

Bruker Avance - 600 MHz

Sample

State anisotropic, Solvent system 90% H2O/10% D2O, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	NOXO1b	[U-13C; U-15N; U-2H]	0.5 (±0.05) mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

3 2D IPAP

Instrument

Bruker Avance - 600 MHz

Sample

State anisotropic, Solvent system 90% H2O/10% D2O, Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	NOXO1b	[U-13C; U-15N; U-2H]	0.5 (±0.05) mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

4 3D HNCO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	NOXO1b	[U-13C; U-15N; U-2H]	0.5 (±0.05) mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

5 3D HNCO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	NOXO1b	[U-13C; U-15N; U-2H]	0.5 (±0.05) mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

6 3D HNCACO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	NOXO1b	[U-13C; U-15N; U-2H]	0.5 (±0.05) mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

7 3D HNCOCA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	NOXO1b	[U-13C; U-15N; U-2H]	0.5 (±0.05) mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

8 3D HNCA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	NOXO1b	[U-13C; U-15N; U-2H]	0.5 (±0.05) mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

9 3D HNCOCACB

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	NOXO1b	[U-13C; U-15N; U-2H]	0.5 (±0.05) mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

10 3D HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	NOXO1b	[U-13C; U-15N; U-2H]	0.5 (±0.05) mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

11 3D HMCGCB

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	NOXO1b	[U-13C; U-15N; U-2H]	0.5 (±0.05) mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

12 3D 1H-15N NOESYHSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	NOXO1b	[U-13C; U-15N; U-2H]	0.5 (±0.05) mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

13 3D 1H-13C NOESYHSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	NOXO1b	[U-13C; U-15N; U-2H]	0.5 (±0.05) mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

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Found 1 Document (0.919 seconds)

BMRB: 17343

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. NOXO1b, : 1 : 15 entities Detail

Experiments performed 13 experiments Detail

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