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BMRB: 17345

2L76

Solution NMR structure of human NFATC2IP ubiquitin-like domain, NFATC2IP_244_338, NESG target HT65A/OCSP target hs00387_244_338/SGC-toronto

Authors

Wu, B., Yee, A., Houliston, S., Doherty, R., Semesi, A., Dhe-Paganon, S., Arrowsmith, C.H.

Assembly

PROTEIN

Entity

1. PROTEIN (polymer, Thiol state: all free), 95 monomers, 10385.02 Da Detail

QGQEDEVVLV EGPTLPETPR LFPLKIRCRA DLVRLPLRMS EPLQSVVDHM ATHLGVSPSR ILLLFGETEL SPTATPRTLK LGVADIIDCV VLTSS

Formula weight

10385.02 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.8 %, Completeness (bb): 97.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	95.8 % (1038 of 1084)	95.5 % (536 of 561)	95.9 % (416 of 434)	96.6 % (86 of 89)
Backbone	97.6 % (539 of 552)	98.4 % (183 of 186)	96.8 % (271 of 280)	98.8 % (85 of 86)
Sidechain	94.7 % (589 of 622)	94.1 % (353 of 375)	96.3 % (235 of 244)	33.3 % (1 of 3)
Aromatic	53.6 % (15 of 28)	64.3 % (9 of 14)	42.9 % (6 of 14)
Methyl	99.3 % (143 of 144)	100.0 % (72 of 72)	98.6 % (71 of 72)

1. NFATC2IP 244 338

QGQEDEVVLV EGPTLPETPR LFPLKIRCRA DLVRLPLRMS EPLQSVVDHM ATHLGVSPSR ILLLFGETEL SPTATPRTLK LGVADIIDCV VLTSS

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	NFATC2IP_244_338	[U-100% 13C; U-100% 15N]	0.5 mM
2	TRIS	[U-100% 2H]	10 mM
3	sodium chloride	natural abundance	300 mM
4	zinc sulphate	natural abundance	10 uM
5	DTT	[U-100% 2H]	10 mM
6	NaN3	natural abundance	0.01 %
7	benzamidine	natural abundance	10 mM
8	inhibitor cocktail	natural abundance	1 na
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
11	NFATC2IP_244_338	[U-100% 13C; U-100% 15N]	0.5 mM
12	TRIS	[U-100% 2H]	10 mM
13	sodium chloride	natural abundance	300 mM
14	zinc sulphate	natural abundance	10 uM
15	DTT	[U-100% 2H]	10 mM
16	NaN3	natural abundance	0.01 %
17	benzamidine	natural abundance	10 mM
18	inhibitor cocktail	natural abundance	1 na
19	H2O	natural abundance	90 %
20	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.15 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.42 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L76, Strand ID: A Detail

Release date

2011-01-04

Citation

Proteins with two SUMO-like domains in chromatin-associated complexes: the RENi (Rad60-Esc2-NIP45) family
Doherty, R., Wu, B., Yee, A., Lemak, A., Houliston, S., Fares, C., Srisalam, S., Bulter, C., Edwards, A.M., Weigelt, J., Dhe-Paganon, S., Arrowsmith, C.H.
BMC Bioinformatics (2005), 6, 22-22, PubMed 15698469 , DOI 10.1186/1471-2105-6-22 , Abstract

Related entities 1. PROTEIN, : 1 : 2 : 1 : 13 entities Detail

Interaction partners 1. PROTEIN, : 4 interactors Detail

More details for 4 interactors identified by 4 citations

Experiments performed 10 experiments Detail

1 3D HNCO