BMRB: 17347

Solution NMR structure of PAP248-286 in 30% TFE

Authors

NANGA, R., BRENDER, J.R., POPOVYCH, N., RAMAMOORTHY, A.

Assembly

PAP248-286

Entity

1. PAP248-286 (polymer, Thiol state: not present), 39 monomers, 4551.383 Da Detail

GIHKQKEKSR LQGGVLVNEI LNHMKRATQI PSYKKLIMY

Formula weight

4551.383 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.4 %, Completeness: 87.9 %, Completeness (bb): 90.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	87.9 % (233 of 265)	87.9 % (233 of 265)
Backbone	90.0 % (72 of 80)	90.0 % (72 of 80)
Sidechain	87.0 % (161 of 185)	87.0 % (161 of 185)
Aromatic	100.0 % (12 of 12)	100.0 % (12 of 12)
Methyl	90.9 % (20 of 22)	90.9 % (20 of 22)

1. entity

GIHKQKEKSR LQGGVLVNEI LNHMKRATQI PSYKKLIMY

Show sample condition

Sample

Solvent system trifluoroethanol/water, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	PAP248-286	natural abundance		1.2 mM
2	sodium phosphate	natural abundance		20 mM
3	D2O	[U-99% 2H]		10 %
4	TFE	[U-99% 2H]		30 %

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Calculated from 10 models in PDB: 2L79, Strand ID: A Detail

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Release date

2011-01-26

Citation

The amyloidogenic SEVI precursor, PAP248-286, is highly unfolded in solution despite an underlying helical tendency
Brender, J.R., Nanga, R., Popovych, N., Soong, R., Macdonald, P.M., Ramamoorthy, A.
Biochim. Biophys. Acta (2011), 1808, 1161-1169, PubMed 21262195 , DOI 10.1016/j.bbamem.2011.01.010 , Abstract

Related entities 1. PAP248-286, : 1 : 8 : 32 entities Detail

Interaction partners 1. PAP248-286, : 3 interactors Detail

Experiments performed 2 experiments Detail