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BMRB: 17354

2LMV

high calcium androcam

Authors

Joshi, M.K., Moran, S.T., MacKenzie, K.R.

Assembly

high calcium androcam

Entity

1. androcam (polymer, Thiol state: all free), 148 monomers, 17014.74 Da Detail

MSELTEEQIA EFKDAFVQFD KEGTGKIATR ELGTLMRTLG QNPTEAELQD LIAEAENNNN GQLNFTEFCG IMAKQMRETD TEEEMREAFK IFDRDGDGFI SPAELRFVMI NLGEKVTDEE IDEMIREADF DGDGMINYEE FVWMISQK

2. CA (non-polymer), 40.078 Da

Total weight

17054.818 Da

Max. entity weight

17014.74 Da

Source organism

Drosophila melanogaster

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.3 %, Completeness: 93.7 %, Completeness (bb): 96.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.7 % (1619 of 1727)	96.7 % (872 of 902)	88.5 % (587 of 663)	98.8 % (160 of 162)
Backbone	96.9 % (857 of 884)	96.4 % (294 of 305)	96.8 % (419 of 433)	98.6 % (144 of 146)
Sidechain	89.8 % (880 of 980)	95.1 % (568 of 597)	80.7 % (296 of 367)	100.0 % (16 of 16)
Aromatic	53.1 % (69 of 130)	100.0 % (65 of 65)	4.7 % (3 of 64)	100.0 % (1 of 1)
Methyl	97.1 % (132 of 136)	95.6 % (65 of 68)	98.5 % (67 of 68)

1. androcam

MSELTEEQIA EFKDAFVQFD KEGTGKIATR ELGTLMRTLG QNPTEAELQD LIAEAENNNN GQLNFTEFCG IMAKQMRETD TEEEMREAFK IFDRDGDGFI SPAELRFVMI NLGEKVTDEE IDEMIREADF DGDGMINYEE FVWMISQK

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	androcam	[U-100% 13C; U-100% 15N]	1.8 mM
2	Calcium chloride	natural abundance	10 mM
3	D2O	natural abundance	10 %
4	H2O	natural abundance	90 %
5	potassium chloride	natural abundance	10 mM
6	TRIS	natural abundance	10 mM
7	DTT	natural abundance	10 mM
8	TSP	natural abundance	0.1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	-0.015 ppm	internal	indirect	0.2514495
¹H	TSP	methyl protons	-0.015 ppm	internal	direct	1.0
¹⁵N	TSP	methyl protons	-0.015 ppm	internal	indirect	0.1013291

Referencing offset: 0.15 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	-0.015 ppm	internal	indirect	0.2514495
¹H	TSP	methyl protons	-0.015 ppm	internal	direct	1.0
¹⁵N	TSP	methyl protons	-0.015 ppm	internal	indirect	0.1013291

Referencing offset: 0.15 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	-0.015 ppm	internal	indirect	0.2514495
¹H	TSP	methyl protons	-0.015 ppm	internal	direct	1.0
¹⁵N	TSP	methyl protons	-0.015 ppm	internal	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	-0.015 ppm	internal	indirect	0.2514495
¹H	TSP	methyl protons	-0.015 ppm	internal	direct	1.0
¹⁵N	TSP	methyl protons	-0.015 ppm	internal	indirect	0.1013291

Referencing offset: -0.47 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LMV, Strand ID: A Detail

Release date

2012-07-24

Citation

¹H, ¹⁵N and ¹³C chemical shifts of the D. melanogaster myosin VI light chain androcam in high calcium
Joshi, M.K., Moran, S., MacKenzie, K.R.
Biomol. NMR Assign. (2013), 7, 171-174, PubMed 22706934 , DOI 10.1007/s12104-012-9403-0 , Abstract

Related entities 1. androcam, : 1 : 4 : 293 entities Detail

Interaction partners 1. androcam, : 26 interactors Detail

More details for 26 interactors identified by 2 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC