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Found 1 Document (1.839 seconds)

BMRB: 17364

2LOZ

The solution structure of the PTB Domain of TENC1 in complex with the peptide of DLC1

Authors

Liu, C., Chen, L., Feng, R., Zhu, G.

Assembly

PTB Domain of TENC1 in complex with the peptide of DLC1

Entity

1. PTB domian (polymer, Thiol state: not reported), 147 monomers, 15963.97 Da Detail

MSTAADLLRQ GAACSVLYLT SVETESLTGP QAVARASSAA LSCSPRPTPA VVHFKVSAQG ITLTDNQRKL FFRRHYPVNS ITFSSTDPQD RRWTNPDGTT SKIFGFVAKK PGSPWENVCH LFAELDPDQP AGAIVTFITK VLLGQRK

2. DLC1 peptide (polymer, Thiol state: not present), 21 monomers, 2240.468 Da Detail

IPEDHKPGTF PKALTNGSFS P

Total weight

18204.438 Da

Max. entity weight

15963.97 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 87.5 %, Completeness: 75.4 %, Completeness (bb): 71.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	75.4 % (1439 of 1908)	81.1 % (800 of 987)	66.7 % (503 of 754)	81.4 % (136 of 167)
Backbone	71.7 % (701 of 978)	82.2 % (272 of 331)	61.3 % (303 of 494)	82.4 % (126 of 153)
Sidechain	80.5 % (876 of 1088)	80.5 % (528 of 656)	80.9 % (338 of 418)	71.4 % (10 of 14)
Aromatic	66.7 % (104 of 156)	75.6 % (59 of 78)	56.6 % (43 of 76)	100.0 % (2 of 2)
Methyl	90.2 % (166 of 184)	91.3 % (84 of 92)	89.1 % (82 of 92)

1. PTB domian

MSTAADLLRQ GAACSVLYLT SVETESLTGP QAVARASSAA LSCSPRPTPA VVHFKVSAQG ITLTDNQRKL FFRRHYPVNS ITFSSTDPQD RRWTNPDGTT SKIFGFVAKK PGSPWENVCH LFAELDPDQP AGAIVTFITK VLLGQRK

2. DLC1 peptide

IPEDHKPGTF PKALTNGSFS P

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	PTB domian	[U-100% 13C; U-100% 15N]	0.6-0.8 mM
2	potassium phosphate	natural abundance	100 mM
3	sodium chloride	natural abundance	100 mM
4	EDTA	natural abundance	1 mM
5	DLC1 peptide	natural abundance	1.2-1.4 mM
6	H2O	natural abundance	90 mM
7	D2O	natural abundance	10 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
8	PTB domian	[U-100% 13C; U-100% 15N]	0.6-0.8 mM
9	DLC1 peptide	natural abundance	1.2-1.4 mM
10	potassium phosphate	natural abundance	100 mM
11	sodium chloride	natural abundance	100 mM
12	EDTA	natural abundance	1 mM
13	D2O	natural abundance	100 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2LOZ, Strand ID: A, B Detail

Release date

2012-06-03

Citation

Solution structure of the phosphotyrosine binding (PTB) domain of human tensin2 protein in complex with deleted in liver cancer 1 (DLC1) peptide reveals a novel peptide binding mode
Chen, L., Liu, C., Ko, F., Xu, N., Ng, I., Yam, J., Zhu, G.
J. Biol. Chem. (2012), 287, 26104-26114, PubMed 22645138 , DOI 10.1074/jbc.M112.360206 , Abstract

Related entities 1. PTB domian, : 2 : 30 entities Detail

Related entities 2. DLC1 peptide, : 2 : 1 : 21 entities Detail

Interaction partners 1. PTB domian, : 35 interactors Detail

More details for 35 interactors identified by 10 citations

Interaction partners 2. DLC1 peptide, : 31 : 1 interactors Detail

More details for 32 interactors identified by 4 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	PTB domian	[U-100% 13C; U-100% 15N]	0.6-0.8 mM
2	potassium phosphate	natural abundance	100 mM
3	sodium chloride	natural abundance	100 mM
4	EDTA	natural abundance	1 mM
5	DLC1 peptide	natural abundance	1.2-1.4 mM
6	H2O	natural abundance	90 mM
7	D2O	natural abundance	10 mM

2 2D 1H-13C HSQC

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
8	PTB domian	[U-100% 13C; U-100% 15N]	0.6-0.8 mM
9	DLC1 peptide	natural abundance	1.2-1.4 mM
10	potassium phosphate	natural abundance	100 mM
11	sodium chloride	natural abundance	100 mM
12	EDTA	natural abundance	1 mM
13	D2O	natural abundance	100 mM

3 3D HNCO

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	PTB domian	[U-100% 13C; U-100% 15N]	0.6-0.8 mM
2	potassium phosphate	natural abundance	100 mM
3	sodium chloride	natural abundance	100 mM
4	EDTA	natural abundance	1 mM
5	DLC1 peptide	natural abundance	1.2-1.4 mM
6	H2O	natural abundance	90 mM
7	D2O	natural abundance	10 mM

4 3D CBCA(CO)NH

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	PTB domian	[U-100% 13C; U-100% 15N]	0.6-0.8 mM
2	potassium phosphate	natural abundance	100 mM
3	sodium chloride	natural abundance	100 mM
4	EDTA	natural abundance	1 mM
5	DLC1 peptide	natural abundance	1.2-1.4 mM
6	H2O	natural abundance	90 mM
7	D2O	natural abundance	10 mM

5 3D HNCACB

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	PTB domian	[U-100% 13C; U-100% 15N]	0.6-0.8 mM
2	potassium phosphate	natural abundance	100 mM
3	sodium chloride	natural abundance	100 mM
4	EDTA	natural abundance	1 mM
5	DLC1 peptide	natural abundance	1.2-1.4 mM
6	H2O	natural abundance	90 mM
7	D2O	natural abundance	10 mM

6 3D H(CCO)NH

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	PTB domian	[U-100% 13C; U-100% 15N]	0.6-0.8 mM
2	potassium phosphate	natural abundance	100 mM
3	sodium chloride	natural abundance	100 mM
4	EDTA	natural abundance	1 mM
5	DLC1 peptide	natural abundance	1.2-1.4 mM
6	H2O	natural abundance	90 mM
7	D2O	natural abundance	10 mM

7 3D C(CO)NH

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	PTB domian	[U-100% 13C; U-100% 15N]	0.6-0.8 mM
2	potassium phosphate	natural abundance	100 mM
3	sodium chloride	natural abundance	100 mM
4	EDTA	natural abundance	1 mM
5	DLC1 peptide	natural abundance	1.2-1.4 mM
6	H2O	natural abundance	90 mM
7	D2O	natural abundance	10 mM

8 3D 1H-15N NOESY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	PTB domian	[U-100% 13C; U-100% 15N]	0.6-0.8 mM
2	potassium phosphate	natural abundance	100 mM
3	sodium chloride	natural abundance	100 mM
4	EDTA	natural abundance	1 mM
5	DLC1 peptide	natural abundance	1.2-1.4 mM
6	H2O	natural abundance	90 mM
7	D2O	natural abundance	10 mM

9 3D HCCH-COSY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
8	PTB domian	[U-100% 13C; U-100% 15N]	0.6-0.8 mM
9	DLC1 peptide	natural abundance	1.2-1.4 mM
10	potassium phosphate	natural abundance	100 mM
11	sodium chloride	natural abundance	100 mM
12	EDTA	natural abundance	1 mM
13	D2O	natural abundance	100 mM

10 3D HCCH-TOCSY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
8	PTB domian	[U-100% 13C; U-100% 15N]	0.6-0.8 mM
9	DLC1 peptide	natural abundance	1.2-1.4 mM
10	potassium phosphate	natural abundance	100 mM
11	sodium chloride	natural abundance	100 mM
12	EDTA	natural abundance	1 mM
13	D2O	natural abundance	100 mM

11 3D 1H-13C NOESY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
8	PTB domian	[U-100% 13C; U-100% 15N]	0.6-0.8 mM
9	DLC1 peptide	natural abundance	1.2-1.4 mM
10	potassium phosphate	natural abundance	100 mM
11	sodium chloride	natural abundance	100 mM
12	EDTA	natural abundance	1 mM
13	D2O	natural abundance	100 mM

12 3D 13C/15N filtered-13C edited NOESY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
8	PTB domian	[U-100% 13C; U-100% 15N]	0.6-0.8 mM
9	DLC1 peptide	natural abundance	1.2-1.4 mM
10	potassium phosphate	natural abundance	100 mM
11	sodium chloride	natural abundance	100 mM
12	EDTA	natural abundance	1 mM
13	D2O	natural abundance	100 mM

nullKeywords DLC1, NMR, PTB, TENC1

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Found 1 Document (1.839 seconds)

BMRB: 17364

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. PTB domian, : 2 : 30 entities Detail

Related entities 2. DLC1 peptide, : 2 : 1 : 21 entities Detail

Interaction partners 1. PTB domian, : 35 interactors Detail

Interaction partners 2. DLC1 peptide, : 31 : 1 interactors Detail

Experiments performed 12 experiments Detail

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