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BMRB: 17365

2L7P

ASHH2 a CW domain

Authors

Kristiansen, P., Hoppmann, V., Thorstensen, T., Aalen, R.B., Aasland, R., Finne, K., Veiseth, S.

Assembly

ASHH2 a CW domain

Entity

1. ASHH2 a CW domain, entity 1 (polymer, Thiol state: free and other bound), 100 monomers, 11201.12 Da Detail

GSRRASVGSE FMVVDVTIED SYSTESAWVR CDDCFKWRRI PASVVGSIDE SSRWICMNNS DKRFADCSKS QEMSNEEINE ELGIGQDEAD AYDCDAAKRG

2. ZN (non-polymer), 65.409 Da

Total weight

11266.529 Da

Max. entity weight

11201.12 Da

Entity Connection

na 4 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS31:SG	2:ZN1:ZN
2	na	sing	1:CYS34:SG	2:ZN1:ZN
3	na	sing	1:CYS56:SG	2:ZN1:ZN
4	na	sing	1:CYS67:SG	2:ZN1:ZN

Source organism

Arabidopsis thaliana

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.0 %, Completeness: 96.7 %, Completeness (bb): 98.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.7 % (1056 of 1092)	97.9 % (555 of 567)	94.7 % (395 of 417)	98.1 % (106 of 108)
Backbone	98.5 % (589 of 598)	98.0 % (201 of 205)	99.0 % (291 of 294)	98.0 % (97 of 99)
Sidechain	95.4 % (561 of 588)	97.8 % (354 of 362)	91.2 % (198 of 217)	100.0 % (9 of 9)
Aromatic	79.3 % (65 of 82)	92.7 % (38 of 41)	63.2 % (24 of 38)	100.0 % (3 of 3)
Methyl	98.7 % (75 of 76)	100.0 % (38 of 38)	97.4 % (37 of 38)

1. entity 1

GSRRASVGSE FMVVDVTIED SYSTESAWVR CDDCFKWRRI PASVVGSIDE SSRWICMNNS DKRFADCSKS QEMSNEEINE ELGIGQDEAD AYDCDAAKRG

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	entity_1	[U-98% 13C; U-98% 15N]	0.8 mM
2	ZINC ION	natural abundance	10 uM
3	DSS	natural abundance	0.2 mM
4	DTT	natural abundance	1.0 mM
5	potassium phosphate	natural abundance	20 mM
6	potassium chloride	natural abundance	50 mM
7	D2O	natural abundance	5 %
8	H2O	natural abundance	95 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L7P, Strand ID: A Detail

Release date

2011-05-01

Citation

The CW domain, a new histone recognition module in chromatin proteins
Hoppmann, V., Thorstensen, T., Kristiansen, P., Veiseth, S.V., Rahman, M.A., Finne, K., Aalen, R., Aasland, R.B.
EMBO J. (2011), 30, 1939-1952, PubMed 21522130 , DOI 10.1038/emboj.2011.108 , Abstract

Related entities 1. ASHH2 a CW domain, entity 1, : 1 : 2 : 1 entities Detail

Interaction partners 1. ASHH2 a CW domain, entity 1, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 18 experiments Detail

1 2D 1H-15N HSQC