BMRBj - BMREntryMaster

Found 1 Document (0.99 seconds)

BMRB: 17370

2L7Q

Solution NMR structure of conjugate transposon protein BVU_1572(27-141) from Bacteroides Vulgatus, Northeast Structural Genomics Consortium Target BvR155.

Authors

Yang, Y., Ramelot, T.A., Cort, J.R., Wang, D., Ciccosanti, C., Janjua, H., Acton, T.B., Xiao, R., Everett, J.K., Montelione, G.T., Kennedy, M.A.

Assembly

protein BVU 1572(27-141)

Entity

1. protein BVU 1572(27-141) (polymer, Thiol state: not available), 248 monomers, 29272.04 Da Detail

MNELDIQQEY PFTVESMPVA DEIAGDETVE IRLEIKPSGN FIGTVYTLRY FQPDGKGSLK MEDGTVLKPN DRYLLNEWKF RLYYTSQSDK EAQTIDLYFE DNWGNLQQLT YDFNGKLEHH HHHHMNELDI QQEYPFTVES MPVADEIAGD ETVEIRLEIK PSGNFIGTVY TLRYFQPDGK GSLKMEDGTV LKPNDRYLLN EWKFRLYYTS QSDKEAQTID LYFEDNWGNL QQLTYDFNGK LEHHHHHH

Formula weight

29272.04 Da

Source organism

Phocaeicola vulgatus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 62.5 %, Completeness: 54.1 %, Completeness (bb): 57.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	54.1 % (1625 of 3002)	53.7 % (840 of 1564)	53.4 % (624 of 1168)	59.6 % (161 of 270)
Backbone	57.8 % (848 of 1468)	58.0 % (291 of 502)	57.0 % (415 of 728)	59.7 % (142 of 238)
Sidechain	51.6 % (911 of 1766)	51.7 % (549 of 1062)	51.0 % (343 of 672)	59.4 % (19 of 32)
Aromatic	39.2 % (135 of 344)	40.1 % (69 of 172)	38.1 % (64 of 168)	50.0 % (2 of 4)
Methyl	53.9 % (123 of 228)	54.4 % (62 of 114)	53.5 % (61 of 114)

1. protein BVU

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	conjugate transposon protein BVU_1572(27-141)	[U-100% 13C; U-100% 15N]	0.53 (±0.05) mM
2	MES	natural abundance	20 (±1.0) mM
3	sodium chloride	natural abundance	100 (±5.0) mM
4	calcium chloride	natural abundance	5 (±0.25) mM
5	sodium azide	natural abundance	0.02 (±0.001) mM
6	DTT	natural abundance	10 (±0.5) mM
7	D2O	natural abundance	10 %
8	H2O	natural abundance	90 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
9	conjugate transposon protein BVU_1572(27-141)	[U-5% 13C; U-100% 15N]	0.48 (±0.05) mM
10	MES	natural abundance	20 (±1.0) mM
11	sodium chloride	natural abundance	100 (±5.0) mM
12	calcium chloride	natural abundance	5 (±0.25) mM
13	DTT	natural abundance	10 (±0.5) mM
14	sodium azide	natural abundance	0.02 (±0.001) mM
15	D2O	natural abundance	10 %
16	H2O	natural abundance	90 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
17	conjugate transposon protein BVU_1572(27-141)	[U-100% 13C; U-100% 15N]	0.53 (±0.05) mM
18	MES	natural abundance	20 (±1.0) mM
19	sodium chloride	natural abundance	100 (±5.0) mM
20	calcium chloride	natural abundance	5 (±0.25) mM
21	sodium azide	natural abundance	0.02 (±0.001) %
22	DTT	natural abundance	10 (±0.5) mM
23	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 4 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.85 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L7Q, Strand ID: A Detail

Release date

2011-01-04

Citation

Solution NMR structure of conjugate transposon protein BVU_1572(27-141) from Bacteroides Vulgatus, Northeast Structural Genomics Consortium Target BvR155
Yang, Y., Ramelot, T.A., Cort, J.R., Wang, D., Ciccosanti, C., Janjua, H., Acton, T.B., Xiao, R., Everett, J.K., Montelione, G.T., Kennedy, M.A.
Not known

Experiments performed 27 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	conjugate transposon protein BVU_1572(27-141)	[U-100% 13C; U-100% 15N]	0.53 (±0.05) mM
2	MES	natural abundance	20 (±1.0) mM
3	sodium chloride	natural abundance	100 (±5.0) mM
4	calcium chloride	natural abundance	5 (±0.25) mM
5	sodium azide	natural abundance	0.02 (±0.001) mM
6	DTT	natural abundance	10 (±0.5) mM
7	D2O	natural abundance	10 %
8	H2O	natural abundance	90 %

2 2D 1H-13C HSQC aliphatic

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	conjugate transposon protein BVU_1572(27-141)	[U-100% 13C; U-100% 15N]	0.53 (±0.05) mM
2	MES	natural abundance	20 (±1.0) mM
3	sodium chloride	natural abundance	100 (±5.0) mM
4	calcium chloride	natural abundance	5 (±0.25) mM
5	sodium azide	natural abundance	0.02 (±0.001) mM
6	DTT	natural abundance	10 (±0.5) mM
7	D2O	natural abundance	10 %
8	H2O	natural abundance	90 %

3 2D 1H-13C HSQC aromatic

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	conjugate transposon protein BVU_1572(27-141)	[U-100% 13C; U-100% 15N]	0.53 (±0.05) mM
2	MES	natural abundance	20 (±1.0) mM
3	sodium chloride	natural abundance	100 (±5.0) mM
4	calcium chloride	natural abundance	5 (±0.25) mM
5	sodium azide	natural abundance	0.02 (±0.001) mM
6	DTT	natural abundance	10 (±0.5) mM
7	D2O	natural abundance	10 %
8	H2O	natural abundance	90 %

4 2D 1H-15N HSQC

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
17	conjugate transposon protein BVU_1572(27-141)	[U-100% 13C; U-100% 15N]	0.53 (±0.05) mM
18	MES	natural abundance	20 (±1.0) mM
19	sodium chloride	natural abundance	100 (±5.0) mM
20	calcium chloride	natural abundance	5 (±0.25) mM
21	sodium azide	natural abundance	0.02 (±0.001) %
22	DTT	natural abundance	10 (±0.5) mM
23	D2O	natural abundance	100 %

5 2D 1H-13C HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
17	conjugate transposon protein BVU_1572(27-141)	[U-100% 13C; U-100% 15N]	0.53 (±0.05) mM
18	MES	natural abundance	20 (±1.0) mM
19	sodium chloride	natural abundance	100 (±5.0) mM
20	calcium chloride	natural abundance	5 (±0.25) mM
21	sodium azide	natural abundance	0.02 (±0.001) %
22	DTT	natural abundance	10 (±0.5) mM
23	D2O	natural abundance	100 %

6 2D 1H-13C HSQC-CT

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
9	conjugate transposon protein BVU_1572(27-141)	[U-5% 13C; U-100% 15N]	0.48 (±0.05) mM
10	MES	natural abundance	20 (±1.0) mM
11	sodium chloride	natural abundance	100 (±5.0) mM
12	calcium chloride	natural abundance	5 (±0.25) mM
13	DTT	natural abundance	10 (±0.5) mM
14	sodium azide	natural abundance	0.02 (±0.001) mM
15	D2O	natural abundance	10 %
16	H2O	natural abundance	90 %

7 3D 1H-15N NOESY

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
9	conjugate transposon protein BVU_1572(27-141)	[U-5% 13C; U-100% 15N]	0.48 (±0.05) mM
10	MES	natural abundance	20 (±1.0) mM
11	sodium chloride	natural abundance	100 (±5.0) mM
12	calcium chloride	natural abundance	5 (±0.25) mM
13	DTT	natural abundance	10 (±0.5) mM
14	sodium azide	natural abundance	0.02 (±0.001) mM
15	D2O	natural abundance	10 %
16	H2O	natural abundance	90 %

8 3D 1H-13C NOESY-aliph

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	conjugate transposon protein BVU_1572(27-141)	[U-100% 13C; U-100% 15N]	0.53 (±0.05) mM
2	MES	natural abundance	20 (±1.0) mM
3	sodium chloride	natural abundance	100 (±5.0) mM
4	calcium chloride	natural abundance	5 (±0.25) mM
5	sodium azide	natural abundance	0.02 (±0.001) mM
6	DTT	natural abundance	10 (±0.5) mM
7	D2O	natural abundance	10 %
8	H2O	natural abundance	90 %

9 3D HNCO

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	conjugate transposon protein BVU_1572(27-141)	[U-100% 13C; U-100% 15N]	0.53 (±0.05) mM
2	MES	natural abundance	20 (±1.0) mM
3	sodium chloride	natural abundance	100 (±5.0) mM
4	calcium chloride	natural abundance	5 (±0.25) mM
5	sodium azide	natural abundance	0.02 (±0.001) mM
6	DTT	natural abundance	10 (±0.5) mM
7	D2O	natural abundance	10 %
8	H2O	natural abundance	90 %

10 3D HNCACB

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	conjugate transposon protein BVU_1572(27-141)	[U-100% 13C; U-100% 15N]	0.53 (±0.05) mM
2	MES	natural abundance	20 (±1.0) mM
3	sodium chloride	natural abundance	100 (±5.0) mM
4	calcium chloride	natural abundance	5 (±0.25) mM
5	sodium azide	natural abundance	0.02 (±0.001) mM
6	DTT	natural abundance	10 (±0.5) mM
7	D2O	natural abundance	10 %
8	H2O	natural abundance	90 %

11 3D CBCA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	conjugate transposon protein BVU_1572(27-141)	[U-100% 13C; U-100% 15N]	0.53 (±0.05) mM
2	MES	natural abundance	20 (±1.0) mM
3	sodium chloride	natural abundance	100 (±5.0) mM
4	calcium chloride	natural abundance	5 (±0.25) mM
5	sodium azide	natural abundance	0.02 (±0.001) mM
6	DTT	natural abundance	10 (±0.5) mM
7	D2O	natural abundance	10 %
8	H2O	natural abundance	90 %

12 3D HNCA

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	conjugate transposon protein BVU_1572(27-141)	[U-100% 13C; U-100% 15N]	0.53 (±0.05) mM
2	MES	natural abundance	20 (±1.0) mM
3	sodium chloride	natural abundance	100 (±5.0) mM
4	calcium chloride	natural abundance	5 (±0.25) mM
5	sodium azide	natural abundance	0.02 (±0.001) mM
6	DTT	natural abundance	10 (±0.5) mM
7	D2O	natural abundance	10 %
8	H2O	natural abundance	90 %

13 3D HN(CO)CA

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	conjugate transposon protein BVU_1572(27-141)	[U-100% 13C; U-100% 15N]	0.53 (±0.05) mM
2	MES	natural abundance	20 (±1.0) mM
3	sodium chloride	natural abundance	100 (±5.0) mM
4	calcium chloride	natural abundance	5 (±0.25) mM
5	sodium azide	natural abundance	0.02 (±0.001) mM
6	DTT	natural abundance	10 (±0.5) mM
7	D2O	natural abundance	10 %
8	H2O	natural abundance	90 %

14 3D HBHA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	conjugate transposon protein BVU_1572(27-141)	[U-100% 13C; U-100% 15N]	0.53 (±0.05) mM
2	MES	natural abundance	20 (±1.0) mM
3	sodium chloride	natural abundance	100 (±5.0) mM
4	calcium chloride	natural abundance	5 (±0.25) mM
5	sodium azide	natural abundance	0.02 (±0.001) mM
6	DTT	natural abundance	10 (±0.5) mM
7	D2O	natural abundance	10 %
8	H2O	natural abundance	90 %

15 3D H(CCO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	conjugate transposon protein BVU_1572(27-141)	[U-100% 13C; U-100% 15N]	0.53 (±0.05) mM
2	MES	natural abundance	20 (±1.0) mM
3	sodium chloride	natural abundance	100 (±5.0) mM
4	calcium chloride	natural abundance	5 (±0.25) mM
5	sodium azide	natural abundance	0.02 (±0.001) mM
6	DTT	natural abundance	10 (±0.5) mM
7	D2O	natural abundance	10 %
8	H2O	natural abundance	90 %

16 3D C(CCO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	conjugate transposon protein BVU_1572(27-141)	[U-100% 13C; U-100% 15N]	0.53 (±0.05) mM
2	MES	natural abundance	20 (±1.0) mM
3	sodium chloride	natural abundance	100 (±5.0) mM
4	calcium chloride	natural abundance	5 (±0.25) mM
5	sodium azide	natural abundance	0.02 (±0.001) mM
6	DTT	natural abundance	10 (±0.5) mM
7	D2O	natural abundance	10 %
8	H2O	natural abundance	90 %

17 3D HCCH-COSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	conjugate transposon protein BVU_1572(27-141)	[U-100% 13C; U-100% 15N]	0.53 (±0.05) mM
2	MES	natural abundance	20 (±1.0) mM
3	sodium chloride	natural abundance	100 (±5.0) mM
4	calcium chloride	natural abundance	5 (±0.25) mM
5	sodium azide	natural abundance	0.02 (±0.001) mM
6	DTT	natural abundance	10 (±0.5) mM
7	D2O	natural abundance	10 %
8	H2O	natural abundance	90 %

18 3D HCCH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	conjugate transposon protein BVU_1572(27-141)	[U-100% 13C; U-100% 15N]	0.53 (±0.05) mM
2	MES	natural abundance	20 (±1.0) mM
3	sodium chloride	natural abundance	100 (±5.0) mM
4	calcium chloride	natural abundance	5 (±0.25) mM
5	sodium azide	natural abundance	0.02 (±0.001) mM
6	DTT	natural abundance	10 (±0.5) mM
7	D2O	natural abundance	10 %
8	H2O	natural abundance	90 %

19 3D CCH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
17	conjugate transposon protein BVU_1572(27-141)	[U-100% 13C; U-100% 15N]	0.53 (±0.05) mM
18	MES	natural abundance	20 (±1.0) mM
19	sodium chloride	natural abundance	100 (±5.0) mM
20	calcium chloride	natural abundance	5 (±0.25) mM
21	sodium azide	natural abundance	0.02 (±0.001) %
22	DTT	natural abundance	10 (±0.5) mM
23	D2O	natural abundance	100 %

20 4D CC-NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
17	conjugate transposon protein BVU_1572(27-141)	[U-100% 13C; U-100% 15N]	0.53 (±0.05) mM
18	MES	natural abundance	20 (±1.0) mM
19	sodium chloride	natural abundance	100 (±5.0) mM
20	calcium chloride	natural abundance	5 (±0.25) mM
21	sodium azide	natural abundance	0.02 (±0.001) %
22	DTT	natural abundance	10 (±0.5) mM
23	D2O	natural abundance	100 %

21 3D 1H-13C HSQC-aromatic

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	conjugate transposon protein BVU_1572(27-141)	[U-100% 13C; U-100% 15N]	0.53 (±0.05) mM
2	MES	natural abundance	20 (±1.0) mM
3	sodium chloride	natural abundance	100 (±5.0) mM
4	calcium chloride	natural abundance	5 (±0.25) mM
5	sodium azide	natural abundance	0.02 (±0.001) mM
6	DTT	natural abundance	10 (±0.5) mM
7	D2O	natural abundance	10 %
8	H2O	natural abundance	90 %

22 2D 1H-13C HSQC aliphatic

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
17	conjugate transposon protein BVU_1572(27-141)	[U-100% 13C; U-100% 15N]	0.53 (±0.05) mM
18	MES	natural abundance	20 (±1.0) mM
19	sodium chloride	natural abundance	100 (±5.0) mM
20	calcium chloride	natural abundance	5 (±0.25) mM
21	sodium azide	natural abundance	0.02 (±0.001) %
22	DTT	natural abundance	10 (±0.5) mM
23	D2O	natural abundance	100 %

23 3D HCCH-COSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
17	conjugate transposon protein BVU_1572(27-141)	[U-100% 13C; U-100% 15N]	0.53 (±0.05) mM
18	MES	natural abundance	20 (±1.0) mM
19	sodium chloride	natural abundance	100 (±5.0) mM
20	calcium chloride	natural abundance	5 (±0.25) mM
21	sodium azide	natural abundance	0.02 (±0.001) %
22	DTT	natural abundance	10 (±0.5) mM
23	D2O	natural abundance	100 %

24 3D HNHA

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
9	conjugate transposon protein BVU_1572(27-141)	[U-5% 13C; U-100% 15N]	0.48 (±0.05) mM
10	MES	natural abundance	20 (±1.0) mM
11	sodium chloride	natural abundance	100 (±5.0) mM
12	calcium chloride	natural abundance	5 (±0.25) mM
13	DTT	natural abundance	10 (±0.5) mM
14	sodium azide	natural abundance	0.02 (±0.001) mM
15	D2O	natural abundance	10 %
16	H2O	natural abundance	90 %

25 2D 1H-15N HSQC 150ppm

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	conjugate transposon protein BVU_1572(27-141)	[U-100% 13C; U-100% 15N]	0.53 (±0.05) mM
2	MES	natural abundance	20 (±1.0) mM
3	sodium chloride	natural abundance	100 (±5.0) mM
4	calcium chloride	natural abundance	5 (±0.25) mM
5	sodium azide	natural abundance	0.02 (±0.001) mM
6	DTT	natural abundance	10 (±0.5) mM
7	D2O	natural abundance	10 %
8	H2O	natural abundance	90 %

26 2D 1H-15N HSQC NH2 only

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	conjugate transposon protein BVU_1572(27-141)	[U-100% 13C; U-100% 15N]	0.53 (±0.05) mM
2	MES	natural abundance	20 (±1.0) mM
3	sodium chloride	natural abundance	100 (±5.0) mM
4	calcium chloride	natural abundance	5 (±0.25) mM
5	sodium azide	natural abundance	0.02 (±0.001) mM
6	DTT	natural abundance	10 (±0.5) mM
7	D2O	natural abundance	10 %
8	H2O	natural abundance	90 %

27 2D 1H-13C HSQC aromatic no CT

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	conjugate transposon protein BVU_1572(27-141)	[U-100% 13C; U-100% 15N]	0.53 (±0.05) mM
2	MES	natural abundance	20 (±1.0) mM
3	sodium chloride	natural abundance	100 (±5.0) mM
4	calcium chloride	natural abundance	5 (±0.25) mM
5	sodium azide	natural abundance	0.02 (±0.001) mM
6	DTT	natural abundance	10 (±0.5) mM
7	D2O	natural abundance	10 %
8	H2O	natural abundance	90 %

nullKeywords Bacteroides Vulgatus, conjugate transposon protein, NESG, NMR structure

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (0.99 seconds)

BMRB: 17370

Sample #1

Sample #2

Sample #3

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Experiments performed 27 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument