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BMRB: 17371

2L7R

Solution NMR structure of N-terminal Ubiquitin-like domain of FUBI, a ribosomal protein S30 precursor from Homo sapiens. NorthEast Structural Genomics consortium (NESG) target HR6166

Authors

Lemak, A., Yee, A., Houliston, S., Semesi, A., Doherty, R., Dhe-Paganon, S., Arrowsmith, C.

Assembly

hs00113

Entity

1. hs00113 (polymer, Thiol state: all free), 93 monomers, 9785.909 Da Detail

MGSSHHHHHH SSGLVPRGSM QLFVRAQELH TFEVTGQETV AQIKAHVASL EGIAPEDQVV LLAGAPLEDE ATLGQCGVEA LTTLEVAGRM LGG

Formula weight

9785.909 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

restrained molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 82.8 %, Completeness: 79.6 %, Completeness (bb): 80.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	79.6 % (792 of 995)	78.9 % (399 of 506)	79.6 % (313 of 393)	83.3 % (80 of 96)
Backbone	80.6 % (445 of 552)	80.4 % (156 of 194)	80.2 % (215 of 268)	82.2 % (74 of 90)
Sidechain	77.9 % (409 of 525)	77.6 % (242 of 312)	78.3 % (162 of 207)	83.3 % (5 of 6)
Aromatic	48.1 % (25 of 52)	50.0 % (13 of 26)	46.2 % (12 of 26)
Methyl	89.2 % (107 of 120)	88.3 % (53 of 60)	90.0 % (54 of 60)

1. hs00113

MGSSHHHHHH SSGLVPRGSM QLFVRAQELH TFEVTGQETV AQIKAHVASL EGIAPEDQVV LLAGAPLEDE ATLGQCGVEA LTTLEVAGRM LGG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	hs00113	[U-13C; U-15N]	0.5 mM
2	mops	natural abundance	10 mM
3	sodium chloride	natural abundance	450 mM
4	ZnSO4	natural abundance	10 mM
5	DTT	natural abundance	10 mM
6	NaN3	natural abundance	0.01 %
7	benzamidine	natural abundance	10 mM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.16 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.16 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L7R, Strand ID: A Detail

Release date

2011-01-23

Citation

Solution NMR structure of human Ubiquitin-like protein hs00113
Lemak, A., Yee, A., Houliston, S., Doherty, R., Semesi, A., Dhe-Paganon, S., Arrowsmith, C.
Not known

Related entities 1. hs00113, : 1 : 689 entities Detail

Experiments performed 8 experiments Detail

1 3D HNCO