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BMRB: 17374

2LJ8

1H, 13C and 15N resonance assignments for ADF/Cofilin from Trypanosoma brucei

Authors

Dai, K., Yuan, G., Liao, S., Zhang, J., Tu, X.

Assembly

ADF/Cofilin from Trypanosoma brucei

Entity

1. ADF/Cofilin from Trypanosoma brucei (polymer, Thiol state: unknown), 144 monomers, 16130.01 Da Detail

MGHHHHHHMA MSGVSVADEC VTALNDLRHK KSRYVIMHIV DQKSIAVKTI GERGANFDQF IEAIDKNVPC YAAFDFEYTT NDGPRDKLIL ISWNPDSGAP RTKMLYSSSR DALVPLTQGF QGIQANDASG LDFEEISRKV KSNR

Formula weight

16130.01 Da

Source organism

Trypanosoma brucei

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.1 %, Completeness: 80.0 %, Completeness (bb): 92.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	80.0 % (1320 of 1650)	77.8 % (666 of 856)	81.5 % (523 of 642)	86.2 % (131 of 152)
Backbone	92.7 % (792 of 854)	92.8 % (271 of 292)	92.2 % (390 of 423)	94.2 % (131 of 139)
Sidechain	70.2 % (654 of 931)	70.0 % (395 of 564)	73.2 % (259 of 354)	0.0 % (0 of 13)
Aromatic	2.2 % (3 of 136)	4.4 % (3 of 68)	0.0 % (0 of 67)	0.0 % (0 of 1)
Methyl	88.9 % (128 of 144)	90.3 % (65 of 72)	87.5 % (63 of 72)

1. ADF Cofilin

MGHHHHHHMA MSGVSVADEC VTALNDLRHK KSRYVIMHIV DQKSIAVKTI GERGANFDQF IEAIDKNVPC YAAFDFEYTT NDGPRDKLIL ISWNPDSGAP RTKMLYSSSR DALVPLTQGF QGIQANDASG LDFEEISRKV KSNR

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	ADF/Cofilin	[U-99% 13C; U-99% 15N]	0.6 mM
2	sodium chloride	natural abundance	100 mM
3	sodium phosphate	natural abundance	20 mM
4	EDTA	natural abundance	2 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.48 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.48 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.22 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LJ8, Strand ID: A Detail

Release date

2011-01-05

Citation

1H, 13C and 15N resonance assignments for a putative ADF/Cofilin from Trypanosoma brucei
Dai, K., Yuan, G., Liao, S., Zhang, J., Tu, X.
Biomol. NMR Assign. (2011), 5, 249-251, PubMed 21523437 , DOI 10.1007/s12104-011-9311-8 , Abstract

Related entities 1. ADF/Cofilin from Trypanosoma brucei, : 1 : 176 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC