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BMRB: 17389

2L81

Solution NMR Structure of the serine-rich domain of hEF1( Enhancer of filamentation 1) from homo sapiens, Northeast Structural Genomics Consortium Target HR5554A

Authors

Liu, G., Xiao, R., Huang, Y.J., Patel, D., Ciccosanti, C.T., Acton, T.B., Tong, S., Everett, J.T., Montelione, G.T.

Assembly

HR5554A

Entity

1. HR5554A (polymer, Thiol state: all free), 176 monomers, 20205.89 Da Detail

MGHHHHHHSH MDKRLFLDPD TAIERLQRLQ QALEMGVSSL MALVTTDWRC YGYMERHINE IRTAVDKVEL FLKEYLHFVK GAVANAACLP ELILHNKMKR ELQRVEDSHQ ILSQTSHDLN ECSWSLNILA INKPQNKCDD LDRFVMVAKT VPDDAKQLTT TINTNAEALF RPGPGS

Formula weight

20205.89 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

distance geometry, simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.8 %, Completeness: 84.5 %, Completeness (bb): 77.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	84.5 % (1750 of 2070)	90.6 % (975 of 1076)	75.4 % (607 of 805)	88.9 % (168 of 189)
Backbone	77.8 % (812 of 1044)	88.9 % (313 of 352)	67.0 % (350 of 522)	87.6 % (149 of 170)
Sidechain	91.5 % (1094 of 1196)	91.4 % (662 of 724)	91.2 % (413 of 453)	100.0 % (19 of 19)
Aromatic	73.3 % (107 of 146)	75.3 % (55 of 73)	70.4 % (50 of 71)	100.0 % (2 of 2)
Methyl	100.0 % (202 of 202)	100.0 % (101 of 101)	100.0 % (101 of 101)

1. HR5554A

MGHHHHHHSH MDKRLFLDPD TAIERLQRLQ QALEMGVSSL MALVTTDWRC YGYMERHINE IRTAVDKVEL FLKEYLHFVK GAVANAACLP ELILHNKMKR ELQRVEDSHQ ILSQTSHDLN ECSWSLNILA INKPQNKCDD LDRFVMVAKT VPDDAKQLTT TINTNAEALF RPGPGS

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details 0.56 mM [U-100% 13C; U-100% 15N] HR5554A, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	HR5554A	[U-100% 13C; U-100% 15N]	0.56 mM
2	H2O	natural abundance	95 mM
3	D2O	natural abundance	5 mM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details 0.56 mM [U-5% 13C; U-100% 15N] HR5554A, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
4	HR5554A	[U-5% 13C; U-100% 15N]	0.56 mM
5	H2O	natural abundance	95 mM
6	D2O	natural abundance	5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
³¹P	DSS	methyl protons	null	indirect	0.4048086

Referencing offset: -0.09 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
³¹P	DSS	methyl protons	null	indirect	0.4048086

Referencing offset: -0.09 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
³¹P	DSS	methyl protons	null	indirect	0.4048086

Referencing offset: 0.06 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L81, Strand ID: A Detail

Release date

2011-01-30

Related entities 1. HR5554A, : 1 : 2 : 20 entities Detail

Interaction partners 1. HR5554A, : 29 interactors Detail

More details for 29 interactors identified by 12 citations

Experiments performed 18 experiments Detail

1 2D 1H-15N HSQC