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BMRB: 17396

2L8E

1H, 13C, and 15N Chemical Shift Assignments for FCS domain from human polyhomeotic homolog 1

Authors

Ilangovan, U., Kim, C.

Assembly

Zn bound FCS domain of hPh1

Entity

1. FCS domain of hPh1 (polymer, Thiol state: all other bound), 49 monomers, 5480.222 Da Detail

GTRGVDSPSA ELDKKANLLK CEYCGKYAPA EQFRGSKRFC SMTCAKRYN

2. ZN (non-polymer), 65.409 Da

Total weight

5545.6313 Da

Max. entity weight

5480.222 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.8 %, Completeness: 91.3 %, Completeness (bb): 89.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	91.3 % (513 of 562)	90.3 % (269 of 298)	92.5 % (198 of 214)	92.0 % (46 of 50)
Backbone	89.7 % (260 of 290)	88.0 % (88 of 100)	90.9 % (130 of 143)	89.4 % (42 of 47)
Sidechain	90.2 % (286 of 317)	88.9 % (176 of 198)	92.2 % (107 of 116)	100.0 % (3 of 3)
Aromatic	100.0 % (44 of 44)	100.0 % (22 of 22)	100.0 % (22 of 22)
Methyl	80.0 % (24 of 30)	80.0 % (12 of 15)	80.0 % (12 of 15)

1. FCS domain of hPh1

GTRGVDSPSA ELDKKANLLK CEYCGKYAPA EQFRGSKRFC SMTCAKRYN

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	Zn bound FCS domain of hPh1	[U-100% 15N]	1.5 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Concentration
4	Zn bound FCS domain of hPh1	[U-95% 13C; U-95% 15N]	1.5 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Concentration
7	Zn bound FCS domain of hPh1	[U-10% 13C; U-99% 15N]	1.5 mM
8	H2O	natural abundance	95 %
9	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.3 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.3 ppm, Outliers: 4 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L8E, Strand ID: A Detail

Release date

2011-05-18

Citation

Identification of nucleic acid binding residues in the FCS domain of the polycomb group protein polyhomeotic
Wang, R., Ilangovan, U., Leal, B.Z., Robinson, A.K., Amann, B.T., Tong, C.V., Berg, J.M., Hinck, A.P., Kim, C.A.
Biochemistry (2011), 50, 4998-5007, PubMed 21351738 , DOI 10.1021/bi101487s , Abstract

Related entities 1. FCS domain of hPh1, : 1 : 2 : 12 entities Detail

Interaction partners 1. FCS domain of hPh1, : 26 interactors Detail

More details for 26 interactors identified by 9 citations

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC