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BMRB: 17398

2L89

A protein from yeast

Authors

Qiu, Y., Zhang, J., Zhang, W.

Assembly

A protein from fission yeast

Entity

1. A protein from fission yeast (polymer), 216 monomers, 24835.92 Da Detail

SADDRLNFGD RILVKAPGYP WWPALLLRRK ETKDSLNTNS SFNVLYKVLF FPDFNFAWVK RNSVKPLLDS EIAKFLGSSK RKSKELIEAY EASKTPPDLK EESSTDLESA DDRLNFGDRI LVKAPGYPWW PALLLRRKET KDSLNTNSSF NVLYKVLFFP DFNFAWVKRN SVKPLLDSEI AKFLGSSKRK SKELIEAYEA SKTPPDLKEE SSTDLE

Formula weight

24835.92 Da

Source organism

Schizosaccharomyces pombe

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 58.8 %, Completeness: 47.7 %, Completeness (bb): 56.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	47.7 % (1260 of 2644)	48.8 % (677 of 1388)	44.4 % (460 of 1036)	55.9 % (123 of 220)
Backbone	56.2 % (712 of 1268)	57.3 % (243 of 424)	55.3 % (355 of 642)	56.4 % (114 of 202)
Sidechain	42.0 % (666 of 1586)	45.0 % (434 of 964)	36.9 % (223 of 604)	50.0 % (9 of 18)
Aromatic	29.2 % (76 of 260)	49.2 % (64 of 130)	7.3 % (9 of 124)	50.0 % (3 of 6)
Methyl	49.5 % (109 of 220)	44.5 % (49 of 110)	54.5 % (60 of 110)

1. A protein from fission yeast

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 4.8

#	Name	Isotope labeling	Concentration
1	a protein from fission yeast		0.6 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	EDTA	natural abundance	2 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 4.8

#	Name	Isotope labeling	Concentration
7	a protein from fission yeast		0.6 mM
8	sodium phosphate	natural abundance	20 mM
9	sodium chloride	natural abundance	100 mM
10	EDTA	natural abundance	2 mM
11	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L89, Strand ID: A Detail

Release date

2011-12-19

Citation

Solution structure of the Pdp1 PWWP domain reveals its unique binding sites for methylated H4K20 and DNA
Qiu, Y., Zhang, W., Zhao, C., Wang, Y., Wang, W., Zhang, J., Zhang, Z., Li, G., Shi, Y., Tu, X., Wu, J.
Biochem. J. (2012), 442, 527-538, PubMed 22150589 , DOI 10.1042/BJ20111885 , Abstract

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC