NMR Spectroscopy and Molecular Dynamics Simulation of r(CCGCUGCGG)2 Reveal a Dynamic UU Internal Loop Found in Myotonic Dystrophy Type 1
Polymer type: polyribonucleotide
Total | 1H | |
---|---|---|
All | 59.3 % (48 of 81) | 59.3 % (48 of 81) |
Suger, PO4 | 63.0 % (34 of 54) | 63.0 % (34 of 54) |
Nucleobase | 51.9 % (14 of 27) | 51.9 % (14 of 27) |
Aromatic | 73.7 % (14 of 19) | 73.7 % (14 of 19) |
1. RNA (5'-R(*CP*CP*GP*CP*UP*GP*CP*GP*G)-3')
CCGCUGCGGSolvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 7.2, Details pH 7.2, 0.1 M NaCl, and 0.05 mM EDTA
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | RNA (5'-R(*CP*CP*GP*CP*UP*GP*CP*GP*G)-3') | natural abundance | 1.5 (±0.1) mM | |
2 | sodium chloride | natural abundance | 100 mM |